Promene trofičkog stanja u reci Dnjepar i njihov uticaj na zajednicu riba

Ispitivane su promene structure zajednica fitoplanktona i riba, kao i koncentracije azota i fosfora u međugraničnoj reci Dnjepar. Konstatovano je da je koncentracija mineralnog azota najvažniji factor akumulacije antropogenog zagađenja nizvodno u ispitivanim delovima reke Dnjepar. Ukupna količina riba se povecala nizvodno sa povećanjem trofičkog statusa reke. U isto vreme prosečna individualne težina se nizvodno smanjivala

Epizootiological Consequences of the Possible Importation of Plague Agent from Manchuria in the Territory of Trans-Baikal Natural Focus

Experimental investigations have demonstrated that proventriculus of Citellophilus tesquorum sungaris (population habitant in the territory of Trans-Baikal region) and Xenopsylla cheopis (classical vector) is blocked in case of the infestation with the plague microbe strain isolated from humans in Manchuria (China) in 5,6 and 15,4 % of the instances, respectively. These ectoparasites transmit plague agent to white mice both when infected with plague microbe at the moment of clustered/group feeding, and when they have their proventriculus blocked at the moment of individual feeding. Taking into consideration ecology of the species of fleas under discussion and sensitivity of the local rodent population to plague microbe, one cannot exclude possibility of the local epizooty occurrence in the territory of Siberian and Far East regions in case of plague agent importation from China

PECULIARITIES OF INTERACTION BETWEEN CITELLOPHILUS TESQUORUM ALTAICUS (IOFF, 1936) FLEA AND YERSINIA PESTIS WITH VARIOUS PLASMID COMPOSITION

Objective of the study is in vitro investigation of mutual relations between Citellophilus tesquorum altaicus and Yersinia pestis with various plasmid composition: influence of the strain on flea alimentary activity and mortality rate, frequency and dynamics of biofilm formation.Materials and methods. C. tesquorum altaicus were infected by three Yersinia pestis strains: virulent triple-plasmid I-3230 isolated in Mongolia, referential for the Tuva focus I-2638 carrying four plasmids (pYT, pYV, pYP, pTP 33) and also selected from it avirulent isogenic clone I-3480 that lost two plasmids (pYV, pYP). Peculiarities of interaction between fleas and Y. pestis strains were estimated through the lens of specimens with «conglomerates» and “blocks” for feeding, the period from infection prior to the beginning of conglomerates’ formation, alimentary activity, and mortality rate of the infected fleas.Results and conclusions. It was revealed that alimentary activity of the infected insects was higher than that of the control group, and the highest – in fleas infected with I-2638 strain. Greater numbers of dead fleas at feeding was noted in specimens inoculated with I-3230 strain. Predominant significance of I-2638 strain was established in C. tesquorum altaicus biofilm formation both as «conglomerates» and “blocks”. I-3480 strain also formed the conglomerates in fleas more actively than I-3230 lacking pTP33 plasmid. Thus, four-plasmid I-2638 strain surpassed triple-plasmid I-3230 and two-plasmid I-3480 strains in reference to all tested indicators except flea mortality rates. It may testify to co-adaptation of Y. pestis and C. tesquorum altaicus from the Tuva plague focus and to the possibility of a pTP33 functional role in enhancement of a biofilm formation in vivo

Comparative analysis of the legal status of testamentary foundations in Russia and foreign countries

Legal rules on a testamentary foundation are a novelty for Russian legislation. Since there were no analogues of this legal institution in Russia before, many law enforcement problems arise. Russian testamentary foundations have largely adopted the provisions on the foundations of the continental legal system. For a better understanding of the legal nature of this subject of law and solving law enforcement problems, it is necessary to analyze the legal status of testamentary foundations in the states where they have existed for a long time, and take into account the positive foreign experienc

Monte Carlo simulations of segregation in Pt-Re catalyst nanoparticles

We have investigated the segregation of Pt atoms to the surfaces of Pt-Re nanoparticles using the Monte Carlo method and Modified Embedded Atom Method potentials that we have developed for Pt-Re alloys. The Pt75Re25 nanoparticles (containing from 586 to 4033 atoms) are assumed to have disordered fcc configurations and cubo-octahedral shapes (terminated by {l_brace}111{r_brace} and {l_brace}100{r_brace} facets), while the Pt50Re50 and Pt25Re75 nanoparticles (containing from 587 to 4061 atoms) are assumed to have disordered hcp configurations and truncated hexagonal bipyramidal shapes (terminated by {l_brace}0001{r_brace} and {l_brace}101 {bar 1}{r_brace} facets). We predict that due to the segregation process the equilibrium Pt-Re nanoparticles would achieve a core-shell structure, with a Pt-enriched shell surrounding a Pt-deficient core. For fcc cubo-octahedral Pt75Re25 nanoparticles, the shells consist of almost 100 at. percent of Pt atoms. Even in the shells of hcp truncated hexagonal bipyramidal Pt50Re50 nanoparticles, the concentrations of Pt atoms exceed 85 at. percent (35 at. percent higher than the overall concentration of Pt atoms in these nanoparticles). Most prominently, all Pt atoms will segregate to the surfaces in the hcp truncated hexagonal bipyramidal Pt25Re75 nanoparticles containing less than 1000 atoms. We also find that the Pt atoms segregate preferentially to the vertex sites, less to edge sites, and least to facet sites on the shell of Pt-Re nanoparticles

Comparative analysis of pain levels, preoperative anxiety, depression and laboratory parameters during uterine surgery

Purpose of the study was to determine the factors influencing the development of uncontrolled pain syndrome in patients who underwent uterine surgery.Цель исследования – установить факторы, влияющие на развитие неконтролируемого болевого синдрома у пациенток, перенесших операции на матке

STUDYING THE POSSIBLE MUTAGENIC PROPERTIES OF NEW MEDICINE ON THE BASIS OF COMPLEX LITHIUM CITRATE, ALUMINUM OXIDE AND POLYMETHILSILOXANE

Aim of the study was to investigate the possible mutagenic properties of a new drug based on a lithium-containing substance – a complex of lithium citrate, polymethylsiloxane and aluminum oxide. Material and methods. Methods for testing mutagenicity using chromosomal aberrations in the bone marrow cells of CBA mice and somatic recombination in Drosophila melanogaster were used. Results. It was shown that a single intragastric administration of drug at a dose of 5000 mg/kg and a fivefold course of administration at a dose of 400 mg/kg to CBA mice did not increase the level of cytogenetic disorders in bone marrow cells. The study of the lithium complex drug in a somatic mosaicism test revealed that the preparation at a dose of 2000 mg/kg does not increase the frequency of mutations in Drosophila melanogaster. Conclusion. A single intragastric administration of the studied drug at a dose of 5000 mg/kg and its course administration (400 mg/kg × 5) do not increase the level of cytogenetic disorders in the bone marrow cells of CBA mice. In the somatic recombination (mosaicism) test system on D. melanogaster, no increase in the appearance of mutant setae and spots on the body and head was observed when using yellow and singed markers. The results of the study indicate that the studied drug does not have mutagenic properties

Investigations of Structural-Functional Aspects of Epizootic Process in Natural Plague Foci in Siberia

Comprehensively studied have been structural elements of ecosystems of Siberian natural plague foci, as well as levels of integration among epizootic process components, and ways of their functional interaction. Application of the complex approach to the surveillance over structural-functional elements of the parasitic system along with investigations of epizootic process dynamics has provided for identification of peculiarities as regards epizootics development, transformation and evolution of population and carrier/vector coenosis structure in time and space. Revealed is the genetic diversity of plague microbe circulating within the bounds of separate foci and zones of focality. Determined is a long-lasting anti-epidemic effect (more than 20 years) of the field desinsection in the Saglinsk meso-focus of the Tuva natural plague focus

Three non-autonomous signals collaborate for nuclear targeting of CrMYC2, a Catharanthus roseus bHLH transcription factor

<p>Abstract</p> <p>Background</p> <p>CrMYC2 is an early jasmonate-responsive bHLH transcription factor involved in the regulation of the expression of the genes of the terpenic indole alkaloid biosynthesis pathway in <it>Catharanthus roseus</it>. In this paper, we identified the amino acid domains necessary for the nuclear targeting of CrMYC2.</p> <p>Findings</p> <p>We examined the intracellular localization of whole CrMYC2 and of various deletion mutants, all fused with GFP, using a transient expression assay in onion epidermal cells. Sequence analysis of this protein revealed the presence of four putative basic nuclear localization signals (NLS). Assays showed that none of the predicted NLS is active alone. Further functional dissection of CrMYC2 showed that the nuclear targeting of this transcription factor involves the cooperation of three domains located in the C-terminal region of the protein. The first two domains are located at amino acid residues 454-510 and 510-562 and contain basic classical monopartite NLSs; these regions are referred to as NLS3 (KRPRKR) and NLS4 (EAERQRREK), respectively. The third domain, between residues 617 and 652, is rich in basic amino acids that are well conserved in other phylogenetically related bHLH transcription factors. Our data revealed that these three domains are inactive when isolated but act cooperatively to target CrMYC2 to the nucleus.</p> <p>Conclusions</p> <p>This study identified three amino acid domains that act in cooperation to target the CrMYC2 transcription factor to the nucleus. Further fine structure/function analysis of these amino acid domains will allow the identification of new NLS domains and will allow the investigation of the related molecular mechanisms involved in the nuclear targeting of the CrMYC2 bHLH transcription factor.</p