Caracterização dos alcalóides em espécie comercializada como Rauwolfia sellowii Müll. Arg.

Rauwolfia sellowii Müll. Arg. was analysed concerning the alkaloid contents. The root bark showed five indole alkaloids: aricine, ajmaline, tetrahydroalstonine, ajmalicine and reserpine. From a species commercially sold as Rauwolfia sellowii were isolated the alkaloids chelerythrine, skimmianine, maculosidine and nitidine. This chemical composition indicates that this species does not belong to the Apocynaceae family. Some alkaloids were analysed by means of the SOS-chromotest as to their genotoxic activity in a prokaryotic system. Skimmianine showed positive response in presence of mixture of metabolization.Foi realizada análise química em espécie autêntica de Rauwolfia sellowii Müll. Arg. e em amostra comercial. O extrato das cascas das raízes da amostra autêntica mostrou a presença dos alcalóides aricina, ajmalina, tetraidroalstonina, ajmalicina e reserpina. Da amostra comercial foram isolados os alcalóides celeritrina, nitidina, es quimianina e maculosidina, indicando tratar-se de uma falsificação. Alguns alcalóides foram testados quanto ao aspecto tóxico-genético, sendo que a esquimianina mostrou resposta positiva

Atividade antimicrobiana de espécies de Hypericum

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Detecção da atividade antiviral de plantas do gênero hypericum sobre o vírus Maedi-visna

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Avaliação da atividade antifúgica de Pterocaulon alopecuroides em amostras de Fonsecaea pedrosol

45

Determination of coumarins from pterocaulon balansae by an ultra-fast liquid chromatography method in topical applications

Coumarins from Pterocaulon balansae Chodat, Asteraceae, have received increasing attention due to their biological activities, especially for the treatment of skin disorders. This study aimed to develop and validate high-performance and ultra-fast liquid chromatography methods for the analytical determination of coumarins in extracts, in topical formulations, as well as in porcine skin after in vitro permeation/retention studies. The chromatographic conditions consisted of a mobile phase with 0.1% formic acid (A) and acetonitrile (B) using a gradient elution; the flow rate and a column oven temperature: HPLC-UV, 1 ml min−1; 30 °C and UFLC-DAD, 0.55 ml min−1; 55 °C for 45 min and 8 min, respectively. The chemical marker 5-methoxy-6,7- methylenedioxycoumarin was used for the construction of the linearity curve in a concentration range from 0.1 to 7.5 μg ml−1. A low matrix effect was observed. The method robustness was confirmed using the Plackett-Burman experimental design. These methodologies proved to be linear, precise, and accurate for the determination of seven coumarins in analytical and bioanalytical samples in both approaches. However, UFLC analysis time was shortened to about one-fourth the time and consumes significantly less eluent, being more eco-friendly in comparison with HPLC

Spectrophotometric determination of coumarins incorporated into nanoemulsions containing pterocaulon balansae extract

This article reports the validation of a spectrophotometric method to estimate coumarins incorporated into nanoemulsions containing Pterocaulon balansae Chodat extract. The quantification was based on the assay of esculin at 327 nm, which presents the same substitution pattern of coumarins isolated from Pterocaulon species. Linear response (R2 > 0.995) was observed over the range of 5.0 to 25.0 μg/mL. The relative standard deviation values for the intra- and inter-days precision were lower than 3.0 %. The recovery ranged from 93.3 % to 104.1 %. The association efficiency was estimated after the determination of free coumarins in the water phase of nanoemulsions obtained after separation through ultrafiltration/centrifugation devices. The coumarins association was approximately 92 %.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Effects of methanol fraction from leaves of Schinus terebinthifolius raddi on nociception and spinal-cord oxidative biomarkers in rats with neuropathic pain

We determined the antioxidant potential of fractions obtained from leaves of Schinus terebinthifolius, a medicinal plant known in Brazil as aroeira, to select the fraction with the best yield and antioxidant performance. These qualities were found in themethanol fraction (MeF), which was administered intraperitoneally (20mg/kg/day) for 3 and 10 days to rats with chronic constriction injury (CCI) of the sciatic nerve, a model of neuropathic pain. The MeF increased the mechanical and thermal thresholds that had been lowered by CCI. In parallel, the lumbosacral spinal cord showed an increase in superoxide dismutase but a decrease in glutathione peroxidase and glutathione-S-transferase activities in saline- and MeF-treated CCI rats. Catalase activity decreased only in salinetreated CCI rats for 10 days. Total thiols decreased in saline- andMeF-treated CCI rats. Ascorbic acid increased in these rats at day 3 but only in saline-treated CCI rats at day 10. No change was found in hydrogen peroxide and lipid hydroperoxide. Open-field and elevated plus-maze tests and blood parameters of liver function did not change.Thus, theMeF from leaves of S. terebinthifolius has an antinociceptive action with no toxic effects, and it affects oxidant biomarkers in the spinal cord of rats with CCI

Envolvimento do sistema dopaminérgico na atividade antidepressiva de Hypericum caprifoliatum Cham. & Schlecht em roedores

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