169 research outputs found

    Automated Teller Machine Ethernet Traffic Identification to Target Forensics Detection of IP Packets

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    Over the last few decades, consumers have become accustom to the convenience of Automatic Teller Machines (ATMs) to transfer funds between accounts, provide account balance information and to withdraw cash from savings, checking, and other account types. Along with the convenience and ease of locating an ATM through mobile bank apps, there has been a significant increase in ATM fraud across the globe. Consumer confidence in the ATM, bank and credit card issuer is greatly impacted by the perceived level of security in ATM transactions and the technology behind them. Confronting the risk associated with ATM fraud and limiting its impact is an important issue that face financial institutions as the sophistication of fraud techniques have advanced. Largely the process behind the verification of these transactions has moved from Plain Old Telephone System (POTS) to Ethernet connections to the processors, banks and card issuers. The attack surface has grown, both in size and complexity. These security risks should be prompting the industry to research all attack surfaces, and this research looks specifically the Ethernet packets that make up these types of transactions. In this research, I investigate the packet structure and predictability within ATM Ethernet traffic. Even with the proliferation of retail ATMs in the most common of retail spaces, this attack vector has received little attention

    Lateral Separation of Macromolecules and Polyelectrolytes in Microlithographic Arrays

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    A new approach to separation of a variety of microscopic and mesoscopic objects in dilute solution is presented. The approach takes advantage of unique properties of a specially designed separation device (sieve), which can be readily built using already developed microlithographic techniques. Due to the broken reflection symmetry in its design, the direction of motion of an object in the sieve varies as a function of its self-diffusion constant, causing separation transverse to its direction of motion. This gives the device some significant and unique advantages over existing fractionation methods based on centrifugation and electrophoresis.Comment: 4 pages with 3 eps figures, needs RevTeX 3.0 and epsf, also available in postscript form http://cmtw.harvard.edu/~deniz

    DNA electrophoresis in designed channels

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    We present a simple description on the electrophoretic dynamics of polyelectrolytes going through designed channels with narrow constrictions of slit geometry. By analyzing rheological behaviours of the stuck chain, which is coupled to the effect of solvent flow, three critical electric fields (permeation field E(per)N1E^{(per)} \sim N^{-1}, deformation field E(def)N3/5E^{(def)} \sim N^{-3/5} and injection field E(inj)N0E^{(inj)} \simeq N^0, with NN polymerization index) are clarified. Between E(per)E^{(per)} and E(inj)E^{(inj)}, the chain migration is dictated by the driven activation process. In particular, at E>E(def)E>E^{(def)}, the stuck chain at the slit entrance is strongly deformed, which enhances the rate of the permeation. From these observations, electrophoretic mobility at a given electric field is deduced, which shows non-monotonic dependence on NN. For long enough chains, mobility increases with NN, in good agreement with experiments. An abrupt change in the electrophoretic flow at a threshold electric field is formally regarded as a nonequilibrium phase transition.Comment: 11 pages, 8 figure

    Gel-Electrophoresis and Diffusion of Ring-Shaped DNA

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    A model for the motion of ring-shaped DNA in a gel is introduced and studied by numerical simulations and a mean-field approximation. The ring motion is mediated by finger-shaped loops (hernias) that move in an amoeba-like fashion around the gel obstructions. This constitutes an extension of previous reptation tube treatments. It is shown that tension is essential for describing the dynamics in the presence of hernias. It is included in the model as long range interactions over stretched DNA regions. The mobility of ring-shaped DNA is found to saturate much as in the well-studied case of linear DNA. Experiments in polymer gels, however, show that the mobility drops exponentially with the DNA ring size. This is commonly attributed to dangling-ends in the gel that can impale the ring. The predictions of the present model are expected to apply to artificial 2D obstacle arrays (W.D. Volkmuth, R.H. Austin, Nature 358,600 (1992)) which have no dangling-ends. In the zero-field case an exact solution of the model steady-state is obtained, and quantities such as the average ring size are calculated. An approximate treatment of the ring dynamics is given, and the diffusion coefficient is derived. The model is also discussed in the context of spontaneous symmetry breaking in one dimension.Comment: 8 figures, LaTeX, Phys. Rev. E - in pres

    A new bond fluctuation method for a polymer undergoing gel electrophoresis

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    We present a new computational methodology for the investigation of gel electrophoresis of polyelectrolytes. We have developed the method initially to incorporate sliding motion of tight parts of a polymer pulled by an electric field into the bond fluctuation method (BFM). Such motion due to tensile force over distances much larger than the persistent length is realized by non-local movement of a slack monomer at an either end of the tight part. The latter movement is introduced stochastically. This new BFM overcomes the well-known difficulty in the conventional BFM that polymers are trapped by gel fibers in relatively large fields. At the same time it also reproduces properly equilibrium properties of a polymer in a vanishing filed limit. The new BFM thus turns out an efficient computational method to study gel electrophoresis in a wide range of the electric field strength.Comment: 15 pages, 11 figure

    Conformation dependence of DNA electrophoretic mobility in a converging channel

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    The electrophoresis of Λ-DNA is observed in a microscale converging channel where the center-of-masses trajectories of DNA molecules are tracked to measure instantaneous electrophoretic (EP) mobilities of DNA molecules of various stretch lengths and conformations. Contrary to the usual assumption that DNA mobility is a constant, independent of field and DNA length in free solution, we find DNA EP mobility varies along the axis in the contracting geometry. We correlate this mobility variation with the local stretch and conformational changes of the DNA, which are induced by the electric field gradient produced by the contraction. A “shish-kebab” model of a rigid polymer segment is developed, which consists of aligned spheres acting as charge and drag centers. The EP mobility of the shish-kebab is obtained by determining the electrohydrodynamic interactions of aligned spheres driven by the electric field. Multiple shish-kebabs are then connected end-to-end to form a freely jointed chain model for a flexible DNA chain. DNA EP mobility is finally obtained as an ensemble average over the shish-kebab orientations that are biased to match the overall stretch of the DNA chain. Using physically reasonable parameters, the model agrees well with experimental results for the dependence of EP mobility on stretch and conformation. We find that the magnitude of the EP mobility increases with DNA stretch, and that this increase is more pronounced for folded conformations.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/77968/1/2813_ftp.pd

    Rectification and Phase Locking for Particles on Two Dimensional Periodic Substrates

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    We show that a novel rectification phenomena is possible for overdamped particles interacting with a 2D periodic substrate and driven with a longitudinal DC drive and a circular AC drive. As a function of DC amplitude, the longitudinal velocity increases in a series of quantized steps with transverse rectification occuring near these transitions. We present a simple model that captures the quantization and rectification behaviors.Comment: 4 pages, 4 postscript figure

    Brownian motion exhibiting absolute negative mobility

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    We consider a single Brownian particle in a spatially symmetric, periodic system far from thermal equilibrium. This setup can be readily realized experimentally. Upon application of an external static force F, the average particle velocity is negative for F>0 and positive for F<0 (absolute negative mobility).Comment: 4 pages, 3 figures, to be published in PR

    Modeling ssDNA electrophoretic migration with band broadening in an entangled or cross-linked network

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    We use a coarse-grained model proposed by Graham and Larson based on the temporary network model by Schieber et al.. [1] to simulate the electrophoretic motion of ssDNA and corresponding band broadening due to dispersion. With dimensionless numbers reflecting the experimental physical properties, we are able to simulate ssDNA behavior under weak to moderate electric field strengths for chains with 8–50 entanglements per chain (∼1000–8500 14base pairs), and model smoothly the transition from reptation to oriented reptation. These results are fitted with an interpolation equation, which allows the user to calculate dimensionless mobilities easily from input parameters characterizing the gel matrix, DNA molecules, and field strengths. Dimensionless peak widths are predicted from mobility fluctuations using the central limit theorem and the assumption that the mobility fluctuations are Gaussian. Using results from previous studies of ssDNA physical properties (effective charge Ξq and Kuhn step length b K ) and sieving matrix properties (pore size or tube diameter a ), we give scaling factors to convert the dimensionless values to “real” experimental values, including the mobility, migration distance, and time. We find that the interpolation equation fits well the experimental data of ssDNA mobilities and peak widths, supporting the validity of the coarse-grained model. The model does not account for constraint release and hernia formation, and assumes that the sieving network is a homogeneous microstructure with no temperature gradients and no peak width due to injection. These assumptions can be relaxed in future work for more accurate prediction.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/56125/1/2783_ftp.pd

    A microfabricated deformability-based flow cytometer with application to malaria

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    Malaria resulting from Plasmodium falciparum infection is a major cause of human suffering and mortality. Red blood cell (RBC) deformability plays a major role in the pathogenesis of malaria. Here we introduce an automated microfabricated “deformability cytometer” that measures dynamic mechanical responses of 10[superscript 3] to 10[superscript 4] individual RBCs in a cell population. Fluorescence measurements of each RBC are simultaneously acquired, resulting in a population-based correlation between biochemical properties, such as cell surface markers, and dynamic mechanical deformability. This device is especially applicable to heterogeneous cell populations. We demonstrate its ability to mechanically characterize a small number of P. falciparum-infected (ring stage) RBCs in a large population of uninfected RBCs. Furthermore, we are able to infer quantitative mechanical properties of individual RBCs from the observed dynamic behavior through a dissipative particle dynamics (DPD) model. These methods collectively provide a systematic approach to characterize the biomechanical properties of cells in a high-throughput manner.National Institutes of Health (U.S.) (Grant R01 HL094270-01A1)National Institutes of Health (U.S.) (Grant 1-R01-GM076689-01)Singapore-MIT Alliance for Research and Technology Cente
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