13 research outputs found
Entwicklung einer Auswertungssoftware zur Anwendung Residualer Dipolarer Kopplungen in der organischen StrukturaufklÀrung
Die Dissertation beschĂ€ftigt sich mit der computerbasierten Analyse von Strukturinformationen aus Residualer Dipolarer Kopplungen in schwach orientierten, kleinen organischen MolekĂŒlen.
Die Implementierung eines neuen Computerprogramms wird vorgestellt. Das RDC-Modul des hotFCHT-Softwarepakets wurde speziell auf die BedĂŒrfnisse der Analyse schwach orientierter, kleiner organischer MolekĂŒle hin entwickelt. Die Software fĂŒhrt eine SVD-basierte Anpassung der RDC-Daten an ein Strukturmodell durch und berechnet daraus den Orientierungstensor. Mit Hilfe dieses Tensors werden QualitĂ€tsfaktoren anhand experimenteller und zurĂŒckgerechneter RDC-Daten ermittelt, und vom Tensor abgeleitete Parameter bestimmt, welche die relative Orientierung des Tensors beschreiben. Eine umfassende Fehlerbetrachtung wird basierend auf Monte-Carlo-Simulationen sowie einer SensitivitĂ€tsanalyse durchgefĂŒhrt. DarĂŒber hinaus wird auch die Beschreibung konformationell flexibler MolekĂŒle als Anpassung eines populationsgewichteten Konformerensembles ermöglicht. Hierbei kann entweder der allgemeine Fall eines dedizierten Orientierungstensors fĂŒr jedes Konformer wie auch die NĂ€herung eines gemeinsamen Orientierungstensors fĂŒr alle Konformere betrachtet werden.
Verschieden Anwendungen der Analyse von RDCs schwach orientierter, kleiner organischer MolekĂŒle werden diskutiert: a) die Bestimmung der unbekannten Relativkonfiguration der Naturstoffe Dibromopalau'amin und 4,6-diacetylhygrophorone A12, b) die gleichzeitige Bestimmung der unbekannten Relativkonfiguration sowie der Konformerpopulationen eines synthetischen Butyrolactons, c) Konformationsanalysen eines photoschaltbaren Organokatalysators sowie eines reaktiven Pd-Komplexes, d) Charakterisierung von Enantiodifferenzierung in chiralen, nicht-racemischen Orientierungsmedien auf der Basis von Homopolypeptiden und AminosĂ€ure-stabilisierten Polyacetylenen
Bi-allelic Loss-of-Function CACNA1B Mutations in Progressive Epilepsy-Dyskinesia.
The occurrence of non-epileptic hyperkinetic movements in the context of developmental epileptic encephalopathies is an increasingly recognized phenomenon. Identification of causative mutations provides an important insight into common pathogenic mechanisms that cause both seizures and abnormal motor control. We report bi-allelic loss-of-function CACNA1B variants in six children from three unrelated families whose affected members present with a complex and progressive neurological syndrome. All affected individuals presented with epileptic encephalopathy, severe neurodevelopmental delay (often with regression), and a hyperkinetic movement disorder. Additional neurological features included postnatal microcephaly and hypotonia. Five children died in childhood or adolescence (mean age of death: 9 years), mainly as a result of secondary respiratory complications. CACNA1B encodes the pore-forming subunit of the pre-synaptic neuronal voltage-gated calcium channel Cav2.2/N-type, crucial for SNARE-mediated neurotransmission, particularly in the early postnatal period. Bi-allelic loss-of-function variants in CACNA1B are predicted to cause disruption of Ca2+ influx, leading to impaired synaptic neurotransmission. The resultant effect on neuronal function is likely to be important in the development of involuntary movements and epilepsy. Overall, our findings provide further evidence for the key role of Cav2.2 in normal human neurodevelopment.MAK is funded by an NIHR Research Professorship and receives funding from the Wellcome Trust, Great Ormond Street Children's Hospital Charity, and Rosetrees Trust. E.M. received funding from the Rosetrees Trust (CD-A53) and Great Ormond Street Hospital Children's Charity. K.G. received funding from Temple Street Foundation. A.M. is funded by Great Ormond Street Hospital, the National Institute for Health Research (NIHR), and Biomedical Research Centre. F.L.R. and D.G. are funded by Cambridge Biomedical Research Centre. K.C. and A.S.J. are funded by NIHR Bioresource for Rare Diseases. The DDD Study presents independent research commissioned by the Health Innovation Challenge Fund (grant number HICF-1009-003), a parallel funding partnership between the Wellcome Trust and the Department of Health, and the Wellcome Trust Sanger Institute (grant number WT098051). We acknowledge support from the UK Department of Health via the NIHR comprehensive Biomedical Research Centre award to Guy's and St. Thomas' National Health Service (NHS) Foundation Trust in partnership with King's College London. This research was also supported by the NIHR Great Ormond Street Hospital Biomedical Research Centre. J.H.C. is in receipt of an NIHR Senior Investigator Award. The research team acknowledges the support of the NIHR through the Comprehensive Clinical Research Network. The views expressed are those of the author(s) and not necessarily those of the NHS, the NIHR, Department of Health, or Wellcome Trust. E.R.M. acknowledges support from NIHR Cambridge Biomedical Research Centre, an NIHR Senior Investigator Award, and the University of Cambridge has received salary support in respect of E.R.M. from the NHS in the East of England through the Clinical Academic Reserve. I.E.S. is supported by the National Health and Medical Research Council of Australia (Program Grant and Practitioner Fellowship)
TITANIA: Model Free Interpretation of Residual Dipolar Couplings in the context of Organic Compounds
The direct use of RDCs as restraint to construct
structures based on RDCs is proposed for organic compounds. It uses the vector and dynamics
information available in multi alignment data sets directly for the joint
determination of conformation and configuration of organic compounds. We show that employing these data even
a flat or random start structure converges into the correctly configured
structure when employing multiple alignment data sets in our iterative
procedure. <br /
Incomplete Data Sets in the Model Free Analysis of Experimental Residual Dipolar Couplings in Small Organic Compounds
Residual dipolar couplings (RDCs)
contain information on the relative arrangement and dynamics of internuclear spin vectors in chemical compounds. The recently introduced software
TITANIA implements the MFA and extracts structure and dynamics parameters directly
from experimental RDCs to facilitate de
novo structure refinement for small organic compounds. Herein, we focus on
the challenges faced with experimentally inaccessible data often encountered
for small organic compounds leading to voids in the RDC matrix and the
concomitant effects on the structure refinement. It is shown that RDC data sets
obtained experimentally from currently available
alignment media and measurement methods are of sufficient quality to allow
relative configuration determination even when the relative configuration of
the analyte is completely unknown.<br /
The influence of electronic modifications on rotational barriers of bis-NHC-complexes as observed by dynamic NMR spectroscopy
Extraction of Distance Restraints from Pure Shift NOE experiments
AbstractNMR techniques incorporating pure shift methods to improve signal resolution have recently attracted much attention, owing to their potential use in studies of increasingly complex molecular systems. Extraction of frequencies from these simplified spectra enables easier structure determination, but only a few of the methods presented provide structural parameters derived from signal integral measurements. In particular, for quantification of the nuclear Overhauser effect (NOE) it is highly desirable to utilize pure shift techniques where signal overlap normally prevents accurate signal integration, to enable measurement of a larger number of interatomic distances. However, robust methods for the measurement of interatomic distances using the recently developed pure shift techniques have not been reported to date.In this work we discuss some of the factors determining the accuracy of measurements of signal integrals in interferogram-based Zangger-Sterk (ZS) pure shift NMR experiments. The ZS broadband homodecoupling technique is used in different experiments designed for quantitative NOE determination from pure shift spectra. It is shown that the techniques studied can be used for quantitative extraction of NOE-derived distance restraints, as exemplified for the test case of strychnine
Engineering of new-to-nature halogenated indigo precursors in plants
Plants are versatile chemists producing a tremendous variety of specialized compounds. Here, we describe the engineering of entirely novel metabolic pathways in planta enabling generation of halogenated indigo precursors as non-natural plant products. Indican (indolyl-ÎČ-d-glucopyranoside) is a secondary metabolite characteristic of a number of dyers plants. Its deglucosylation and subsequent oxidative dimerization leads to the blue dye, indigo. Halogenated indican derivatives are commonly used as detection reagents in histochemical and molecular biology applications; their production, however, relies largely on chemical synthesis. To attain the de novo biosynthesis in a plant-based system devoid of indican, we employed a sequence of enzymes from diverse sources, including three microbial tryptophan halogenases substituting the amino acid at either C5, C6, or C7 of the indole moiety. Subsequent processing of the halotryptophan by bacterial tryptophanase TnaA in concert with a mutant of the human cytochrome P450 monooxygenase 2A6 and glycosylation of the resulting indoxyl derivatives by an endogenous tobacco glucosyltransferase yielded corresponding haloindican variants in transiently transformed Nicotiana benthamiana plants. Accumulation levels were highest when the 5-halogenase PyrH was utilized, reaching 0.93 ±0.089mg/g dry weight of 5-chloroindican. The identity of the latter was unambiguously confirmed by NMR analysis. Moreover, our combinatorial approach, facilitated by the modular assembly capabilities of the GoldenBraid cloning system and inspired by the unique compartmentation of plant cells, afforded testing a number of alternative subcellular localizations for pathway design. In consequence, chloroplasts were validated as functional biosynthetic venues for haloindican, with the requisite reducing augmentation of the halogenases as well as the cytochrome P450 monooxygenase fulfilled by catalytic systems native to the organelle. Thus, our study puts forward a viable alternative production platform for halogenated fine chemicals, eschewing reliance on fossil fuel resources and toxic chemicals. We further contend that in planta generation of halogenated indigoid precursors previously unknown to nature offers an extended view on and, indeed, pushes forward the established frontiers of biosynthetic capacity of plants
Engineering of a plant isoprenyl diphosphate synthase for development of irregular coupling activity
We performed mutagenesis on a regular isoprenyl diphosphate synthase (IDS), neryl diphosphate synthase from Solanum lycopersicum (SlNPPS), that has a structurally related analogue performing non-head-to-tail coupling of two dimethylallyl diphosphate (DMAPP) units, lavandulyl diphosphate synthase from Lavandula x intermedia (LiLPPS). Wild-type SlNPPS catalyses regular coupling of isopentenyl diphosphate (IPP) and DMAPP in cis-orientation resulting in the formation of neryl diphosphate. However, if the enzyme is fed with DMAPP only, it is able to catalyse the coupling of two DMAPP units and synthesizes two irregular monoterpene diphosphates; their structures were elucidated by the NMR analysis of their dephosphorylation products. One of the alcohols is lavandulol. The second compound is the trans-isomer of planococcol, the first example of an irregular cyclobutane monoterpene with this stereochemical configuration. The irregular activity of SlNPPS constitutes 0.4% of its regular activity and is revealed only if the enzyme is supplied with DMAPP in absence of IPP. The exchange of asparagine 88 for histidine considerably enhanced the non-head-to-tail coupling. While still only observed in the absence of IPP, irregular activity of the mutant reaches 13.1 % of its regular activity. The obtained results prove that regular IDS are promising starting points for protein engineering aiming at the development of irregular activities and leading to novel monoterpene structures
Probing Long-Range Anisotropic Interactions: a General and Sign-Sensitive Strategy to Measure 1 H-1 H Residual Dipolar Couplings as a Key Advance for Organic Structure Determination
International audienceResidual dipolar couplings (RDCs) are amongst the most powerful NMR parameters for organic structure elucidation. In order to maximize their effectiveness in increasingly complex cases such as flexible compounds, a maximum of RDCs between nuclei sampling a large distribution of orientations is needed, including sign information. For this, the easily accessible one-bond 1 H-13 C RDCs alone often fall short. Long-range 1 H-1 H RDCs are both abundant and typically sample highly complementary orientations, but accessing them in a sign-sensitive way has been severely obstructed due to the overflow of 1 H-1 H couplings. Here, we present a generally applicable strategy that allows the measurement of a large number of 1 H-1 H RDCs, including their signs, which is based on a combination of an improved PSYCHEDELIC method and a new selective constant-time ÎČ-COSY experiment. The potential of 1 H-1 H RDCs to better determine molecular alignment and to discriminate between enantiomers and diastereomers is demonstrated