Dendrochronological data from twelve countries proved definite growth response of black alder (Alnus glutinosa [L.] Gaertn.) to climate courses across its distribution range

Black alder (Alnus glutinosa [L.] Gaertn.) is an important component of riparian and wetland ecosystems in Europe. However, data on the growth of this significant broadleaved tree species is very limited. Presently, black alder currently suffers from the pathogen Phytophthora and is particularly threatened by climate change. The objective of this study was to focus on the impact of climatic variables (precipitation, temperature, extreme climatic events) on the radial growth of alder across its geographic range during the period 1975–2015. The study of alder stands aged 46–108 years was conducted on 24 research plots in a wide altitude range (85–1015 m) in 12 countries of Europe and Asia. The most significant months affecting alder radial growth were February and March, where air temperatures are more significant than precipitation. Heavy frost and extreme weather fluctuations in the first quarter of the year were the main limiting factors for diameter increment. Within the geographical setting, latitude had a higher effect on radial growth compared to longitude. However, the most important variable concerning growth parameters was altitude. The temperature’s effect on the increment was negative in the lowlands and yet turned to positive with increasing altitude. Moreover, growth sensitivity to precipitation significantly decreased with the increasing age of alder stands. In conclusion, the growth variability of alder and the number of negative pointer years increased with time, which was caused by the ongoing climate change and also a possible drop in the groundwater level. Riparian alder stands well supplied with water are better adapted to climatic extremes compared to plateau and marshy sites

Mitochondrion of oxymonads

Oxymonads are among the last larger eukaryotic groups in which mitochondrion-like organelles were not detected. Among almost one million transcriptome sequences of oxymonad Monocercomonoides we found sequences for only three proteins commonly associated with mitochondrion-like organelles - pyruvate:ferredoxin oxidoreductase (PFO), [FeFe]hydrogenase and pyridine:nucleotide transhydrogenase (PNT). Out of these tree proteins PNT is most closely associated with mitochondrion-like organelles. Although the transcriptome data set is quite large we did not detect any other mitochondrial proteins including proteins for synthesis of FeS clusters and proteins associated with mitochondrial protein import. In the phylogenetic tree, PNT from Monocercomonoides formed a clade with sequences of other eukaryotes. This suggests that PNT of Monocercomonoides is likely of mitochondrial origin; however, the possibility that Monocercomonoides acquired PNT by lateral gene transfer cannot be excluded. We tried to localize PNT in the cell of Monocercomonoides by immunofluorescent microscopy but the results were difficult to interpret. We could not prove the presence of mitochondrion-like organelle in Monocercomonoides. If there is any such organelle, it is highly probable that this organelle is very reduced because we did not find..

Writing about electronic dance music in genre specific media

Liberal Arts and Humanities - Creative ModuleStudium humanitní vzdělanosti - Kreativní modulFakulta humanitních studiíFaculty of Humanitie

How the czech music magazines contributed to the process of comercionalization of electronic dance music?

This diploma thesis deals with the role of a printed music media in the process of commercialization of electronic dance music in the Czech Republic in the early 2000s. The research is based on the theoretical approach of subcultural studies, more specifically on the Chicago school. The theoretical part is focused on the definition of terms such as subculture, moral panic, subcultural capital and commercialization. The empirical part is focused on qualitative research of selected media, consisting of content analysis of contemporary periodicals and semi-structured interviews with selected media editors. In total, 134 issues were analyzed and four interviews were conducted with respondents as part of the research. The results show the role of selected magazines as subcultural media, which significantly contributed to the development of electronic dance music in our country. Using the obtained results, the work defines the main events and meanings by which the selected media influenced the dance music scene of that time. 1Tato diplomová práce se zabývá rolí tištěných hudebních médií v procesu komercionalizace elektronické taneční hudby v České republice v nultých letech. Vlastní výzkum se opírá o teoretický přístup subkulturálních studií, přesněji o Chicagskou školu. Teoretická část je zaměřena na definici pojmů jako subkultura, morální panika, subkulturní kapitál a komercionalizace. Empirická část je zaměřena na kvalitativní výzkum vybraných médií složený z obsahové analýzy dobových periodik a polostrukturovaných rozhovorů s vybranými redaktory médií. Celkem bylo v rámci výzkumu analyzováno 134 vydání a byli realizovány čtyři rozhovory s respondenty. Ze získaných výsledků vyplývá role vybraných časopisů jako subkulturních médií, které se významně zasloužili za rozvoj elektronické taneční hudby u nás. Za využití získaných výsledků práce definuje hlavní události a významy, kterými vybraná média ovlivňovala tehdejší scénu taneční hudby. 1Department of Media StudiesKatedra mediálních studiíFakulta sociálních vědFaculty of Social Science

Anaerobic mitochondrion-like organelles of Excavata

Department of ParasitologyKatedra parazitologieFaculty of SciencePřírodovědecká fakult

How the czech music magazines contributed to the process of comercionalization of electronic dance music?

This diploma thesis deals with the role of a printed music media in the process of commercialization of electronic dance music in the Czech Republic in the early 2000s. The research is based on the theoretical approach of subcultural studies, more specifically on the Chicago school. The theoretical part is focused on the definition of terms such as subculture, moral panic, subcultural capital and commercialization. The empirical part is focused on qualitative research of selected media, consisting of content analysis of contemporary periodicals and semi-structured interviews with selected media editors. In total, 134 issues were analyzed and four interviews were conducted with respondents as part of the research. The results show the role of selected magazines as subcultural media, which significantly contributed to the development of electronic dance music in our country. Using the obtained results, the work defines the main events and meanings by which the selected media influenced the dance music scene of that time.

Iron-Sulfur cluster assembly in Monocercomonoides exilis

In the search for the mitochondrion of oxymonads, DNA of Monocercomonoides exilis - an oxymonad isolated from the gut of Chinchilla, was isolated and its genome was sequenced. Sequencing resulted in a fairly complete genome which was extensively searched or genes for mitochondrion related proteins, but no reliable candidate for such gene was identified. Even genes for the ISC pathway, which is responsible for Fe-S cluster assembly and considered to be the only essential function of reduced mitochondrion-like organelles (MROs), were absent. Instead, we were able to detect the presence of a SUF pathway which functionally replaced the ISC pathway. Closer examination of the SUF pathway based on heterologous localisation revealed that this pathway localised in the cytosol. In silico analysis showed that SUF genes are highly conserved at the level of secondary and tertiary structure and most catalytic residues and motifs are present in their sequences. The functionality of these proteins was further indirectly confirmed by complementation experiments in Escherichia coli where SUF proteins of M. exilis were able to restore at least partially Fe-S cluster assembly of strains deficient in the SUF and ISC pathways. We also proved by bacterial adenylate cyclase two-hybrid system that SufB and SufC can form..

Iron-Sulfur cluster assembly in Monocercomonoides exilis

In the search for the mitochondrion of oxymonads, DNA of Monocercomonoides exilis - an oxymonad isolated from the gut of Chinchilla, was isolated and its genome was sequenced. Sequencing resulted in a fairly complete genome which was extensively searched or genes for mitochondrion related proteins, but no reliable candidate for such gene was identified. Even genes for the ISC pathway, which is responsible for Fe-S cluster assembly and considered to be the only essential function of reduced mitochondrion-like organelles (MROs), were absent. Instead, we were able to detect the presence of a SUF pathway which functionally replaced the ISC pathway. Closer examination of the SUF pathway based on heterologous localisation revealed that this pathway localised in the cytosol. In silico analysis showed that SUF genes are highly conserved at the level of secondary and tertiary structure and most catalytic residues and motifs are present in their sequences. The functionality of these proteins was further indirectly confirmed by complementation experiments in Escherichia coli where SUF proteins of M. exilis were able to restore at least partially Fe-S cluster assembly of strains deficient in the SUF and ISC pathways. We also proved by bacterial adenylate cyclase two-hybrid system that SufB and SufC can form..