32 research outputs found
Toxicology and farmacology of phosphamidon
Fosfamidon izaziva tipiÄne simptome otrovanja antiholinesterazama. Subletalne doze fosfamidona proizvode kod miÅ”eva i Å”takora znaÄajnu ali relativno kratkotrajnu inhibiciju kolinesteraze u krvi i mozgu. Antiholinesteraznom dejstvu fosfamidona može se pripisati i najveÄi broj njegovih farmakoloÅ”kih efekata na anestetisanim životinjama i izolovanim organima. Istraženo je terapeutsko dejstvo atropina i TMB-4, danih odvojeno ili zajedno, na životinjama otrovanim fosfamidonom. UtvrÄeno je da je njihovo terapeutska dejstvo mnogo jaÄe kada se apliciraju simultano nego kad se daju odvojeno.Phosphamidon produces typical symptoms of anticholinesterase poisoning. Sublethal doses of phosphamidon given to mice and rats produce a pronounced but comparatively short cholinesterase inhibition in the blood and brain. The highest number of the therapeutical and pharmacological effects of phosphamidon observed both on anaesthetized animals and isolated organs can be attributed to the anticholinesterase activity of phosphamidon. The therapeutic effect of atropine and TMB-4 is studied. When given simultaneously, their therapeutic effect is much higher than when given separately
Polymerase chain reaction in the identification of periodontopathogens: A reliable and satisfactory method?
Aggregatibacter actinomycetemcomitans is considered one of the bacterial species of etiological importance in periodontitis. The aim of this study was to evaluate the serotype of A. actinomycetemcomitans in the subgingival biofilm in subjects with periodontal health and disease. Pooled samples of subgingival plaque were taken for culture-based identification of microorganisms. Colonies suspected to be A. actinomycetemcomitans were selected for molecular identification using either multiplex or conventional PCR in serotype-specific genotyping and 16S rRNA gene sequencing. In silico analysis showed that most selected colonies belong to the genus Campylobacter, although positive signals for serotypes of A. actinomycetemcomitans were obtained with these samples. Identification of A. actinomycetemcomitans by conventional PCR for 16S rRNA with one species-specific and one universal primer was inconclusive because an almost identical signal with Campylobacter gracilis was obtained. Although PCR-based methods for the identification of A. actinomycetemcomitans are more rapid, sequencing should not be omitted. [Projekat Ministarstva nauke Republike Srbije, br. 41008 and br. 173048
Polymerase chain reaction in the identification of periodontopathogens: A reliable and satisfactory method?
Aggregatibacter actinomycetemcomitans is considered one of the bacterial species of etiological importance in periodontitis. The aim of this study was to evaluate the serotype of A. actinomycetemcomitans in the subgingival biofilm in subjects with periodontal health and disease. Pooled samples of subgingival plaque were taken for culture-based identification of microorganisms. Colonies suspected to be A. actinomycetemcomitans were selected for molecular identification using either multiplex or conventional PCR in serotype-specific genotyping and 16S rRNA gene sequencing. In silico analysis showed that most selected colonies belong to the genus Campylobacter, although positive signals for serotypes of A. actinomycetemcomitans were obtained with these samples. Identification of A. actinomycetemcomitans by conventional PCR for 16S rRNA with one species-specific and one universal primer was inconclusive because an almost identical signal with Campylobacter gracilis was obtained. Although PCR-based methods for the identification of A. actinomycetemcomitans are more rapid, sequencing should not be omitted
The Surface of Sodium Tungsten Bronze Electrodes in Acid Solutions
At eledtrddes of sodium tungsten bronzes Ā· of CJWic> syi;r;imetpy
(0:25 hydraW,d\u27 laye~ of rion-stoiehiotnetric oxideĀ· is.Jormed, On Ā·the \u27Ā·base o0f comwnĀ·ative considerations of results of electrochemical and: .some ; othevr me:;i~u:. . remerits the structure ā¢of the Ā·Ā·surface layer is .ā¢ ptQposed,Ā· ,a_nd, the thiekfiess\u27 . of . the. isur\u27:flace layerā¢ eS>timated,; ā¢Rossiblgru.eNplanati-on:s.ef\u27 ā¢the natureā¢ of Ā· the rest potential arid Ā·N ~ .log iĀ· ,relationship :are po1nted \u27 out; On the base\u27 .of study of ā¢ kinetics. and Ā· mechanism,. of some simple electron exchange reactions . it \u27is concluded Ā·:that elei:;trical properties of the surface layer are close toĀ· metallic
Zinc(II) Complexes with Dimethyl 2,2 '-Bipyridine-4,5-dicarboxylate: Structure, Antimicrobial Activity and DNA/BSA Binding Study
Two zinc(II) complexes with dimethyl 2,2 '-bipyridine-4,5-dicarboxylate (py-2py) of the general formula [Zn(py-2py)X-2], X = Cl- (1) and Br- (2) were synthesized and characterized by NMR, IR and UV-Vis spectroscopy and single-crystal X-ray diffraction analysis. Complexes 1 and 2 are isostructural and adopt a slightly distorted tetrahedral geometry with values of tetrahedral indices tau(4) and tau'(4) in the range of 0.80-0.85. The complexes were evaluated for their in vitro antimicrobial activity against two bacterial (Pseudomonas aeruginosa and Staphylococcus aureus) and two fungal strains (Candida albicans and Candida parapsilosis), while their cytotoxicity was tested on the normal human lung fibroblast cell line (MRC-5) and the model organism Caenorhabditis elegans. Complex 1 showed moderate activity against both Candida strains. However, this complex was twofold more cytotoxic compared to complex 2. The complexes tested had no effect on the survival rate of C. elegans. Complex 2 showed the ability to inhibit filamentation of C. albicans, while complex 1 was more effective than complex 2 in inhibiting biofilm formation. The interactions of complexes 1 and 2 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) were studied to evaluate their binding affinity toward these biomolecules
Mononuclear gold(III) complexes with phenanthroline ligands as efficient inhibitors of angiogenesis: A comparative study with auranofin and sunitinib
Gold(III) complexes with 1,7- and 4,7-phenanthroline ligands, [AuCl3(1,7-phen-kappa N7)] (1) and [AuCl3(4,7-phen-kappa N4)] (2) were synthesized and structurally characterized by spectroscopic (NMR, IR and UV-vis) and single crystal X-ray diffraction techniques. In these complexes, 1,7- and 4,7-phenanthrolines are monodentatedly coordinated to the Au(III) ion through the N7 and N4 nitrogen atoms, respectively. In comparison to the clinically relevant anti-angiogenic compounds auranofin and sunitinib, gold(III)-phenanthroline complexes showed from 1.5- to 20-fold higher anti-angiogenic potential, and 13- and 118-fold lower toxicity. Among the tested compounds, complex 1 was the most potent and may be an excellent anti-angiogenic drug candidate, since it showed strong anti-angiogenic activity in zebrafish embryos achieving IC50 value (concentration resulting in an anti-angiogenic phenotype at 50% of embryos) of 2.89 mu M, while had low toxicity with LC50 value (the concentration inducing the lethal effect of 50% embryos) of 128 mu M. Molecular docking study revealed that both complexes and ligands could suppress angiogenesis targeting the multiple major regulators of angiogenesis, such as the vascular endothelial growth factor receptor (VEGFR-2), the matrix metalloproteases (MMP-2 and MMP-9), and thioredoxin reductase (TrxR1), where the complexes showed higher binding affinity in comparison to ligands, and particularly to auranofin, but comparable to sunitinib, an anti-angiogenic drug of clinical relevance.Related to published version: [https://imagine.imgge.bg.ac.rs/handle/123456789/1011]This is the peer reviewed version of the paper: PaviÄ, A., GliÅ”iÄ, B., VojnoviÄ, S., Warzajtis, B., SaviÄ, N. D., AntiÄ, M., RadenkoviÄ, S., JanjiÄ, G. V., NikodinoviÄ-RuniÄ, J., Rychlewska, U., & Djuran, M. I. (2017). Mononuclear gold(III) complexes with phenanthroline ligands as efficient inhibitors of angiogenesis: A comparative study with auranofin and sunitinib. Journal of Inorganic Biochemistry, 174, 156ā168. [https://doi.org/10.1016/j.jinorgbio.2017.06.009
Supplementary data for article : Pavic, A.; GliÅ”iÄ, B. Ä.; Vojnovic, S.; Warżajtis, B.; SaviÄ, N. D.; AntiÄ, M.; RadenkoviÄ, S.; JanjiÄ, G. V.; Nikodinovic-Runic, J.; Rychlewska, U.; et al. Mononuclear Gold(III) Complexes with Phenanthroline Ligands as Efficient Inhibitors of Angiogenesis: A Comparative Study with Auranofin and Sunitinib. Journal of Inorganic Biochemistry 2017, 174, 156ā168. https://doi.org/10.1016/j.jinorgbio.2017.06.009
Supplementary material for: [https://doi.org/10.1016/j.jinorgbio.2017.06.009]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2496]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3110
Supplementary material for the article: SaviÄ, N. D.; Vojnovic, S.; GliÅ”iÄ, B. Ä.; Crochet, A.; Pavic, A.; JanjiÄ, G. V.; PekmezoviÄ, M.; Opsenica, I. M.; Fromm, K. M.; Nikodinovic-Runic, J.; et al. Mononuclear Silver(I) Complexes with 1,7-Phenanthroline as Potent Inhibitors of Candida Growth. Eur. J. Med. Chem. 2018, 156, 760ā773. https://doi.org/10.1016/j.ejmech.2018.07.049
Supplementary data for: [https://doi.org/10.1016/j.ejmech.2018.07.049]Research data for this article: [https://www.ccdc.cam.ac.uk/structures/search?id=doi:10.5517/ccdc.csd.cc1zdr7c&sid=DataCite]Research data for this article: [https://www.ccdc.cam.ac.uk/structures/search?id=doi:10.5517/ccdc.csd.cc1zdr6b&sid=DataCite]Research data for this article: [https://www.ccdc.cam.ac.uk/structures/search?id=doi:10.5517/ccdc.csd.cc1zdr59&sid=DataCite]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2213]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/2993
Supplementary data for article : Pavic, A.; GliÅ”iÄ, B. Ä.; Vojnovic, S.; Warżajtis, B.; SaviÄ, N. D.; AntiÄ, M.; RadenkoviÄ, S.; JanjiÄ, G. V.; Nikodinovic-Runic, J.; Rychlewska, U.; et al. Mononuclear Gold(III) Complexes with Phenanthroline Ligands as Efficient Inhibitors of Angiogenesis: A Comparative Study with Auranofin and Sunitinib. Journal of Inorganic Biochemistry 2017, 174, 156ā168. https://doi.org/10.1016/j.jinorgbio.2017.06.009
Supplementary material for: [https://doi.org/10.1016/j.jinorgbio.2017.06.009]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2496]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3110