4 research outputs found
Anatomy of a lactococcal phage tail
Bacteriophages of the Siphoviridae family utilize a long noncontractile tail to recognize, adsorb to, and inject DNA into their bacterial host. The tail anatomy of the archetypal Siphoviridae X has been well studied, in contrast to phages infecting gram-positive bacteria. This report outlines a detailed anatomical description of a typical member of the Siphoviridae infecting a gram-positive bacterium. The tail superstructure of the lactococcal phage Tuc2009 was investigated using N-terminal protein sequencing, Western blotting, and immunogold transmission electron microscopy, allowing a tangible path to be followed from gene sequence through encoded protein to specific architectural structures on the Tuc2009 virion. This phage displays a striking parity with X with respect to tail structure, which reenforced a model proposed for Tuc2009 tail architecture. Furthermore, comparisons with X and other lactococcal phages allowed the specification of a number of genetic submodules likely to encode specific tail structures.</p
Taxonomy of prokaryotic viruses: update from the ICTV bacterial and archaeal viruses subcommittee.
International audienc