Results about the damages caused from parasitic disease of Gyrodactyosis in the fingerlings of the rainbow trout (O.mykiss)

The monogenean Gyrodactylus spp. is an important parasite of farmed fish in Europe because of the heavy damages and losses that induce. For this reason one of the main representative, Gyrodactylus salaris is listed in List III of pathogen in the Fish Health Directive 91/67/EEC. During 2013 are collected in Pogradec 200 fingerlings of rainbow trout (Oncorhynchus mykiss) to the purpose of identifying the presence of Gyrodactylus spp. parasite. The parasite was mostly observed on the back of the fish and mostly in the period from March to July because of the favoritism that comes from the water temperature.Keywords: Oncorhynchus mykiss, Gyrodactylus salaris, Gyrodactylus spp,parasite, fish, etc

Results about the damages caused from parasitic disease of Gyrodactyosis in the fingerlings of the rainbow trout (O.mykiss)

The monogenean Gyrodactylus spp. is an important parasite of farmed fish in Europe because of the heavy damages and losses that induce. For this reason, one of the main representatives, Gyrodactylus salaris is listed in List III of pathogens in the Fish Health Directive 91/67/EEC. During 2013 are collected in Pogradec 200 fingerlings of rainbow trout (Oncorhynchus mykiss) to the purpose of identifying the presence of Gyrodactylus spp. parasite. The parasite was mostly observed on the back of the fish and mostly in the period from March to July because of the favoritism that comes from the water temperature

Trichinelosis in Animals

Trichinellosis (also trichinosis) in animals is caused by nematodes (roundworms) of the family Trichinellidae Ward, 1970. Family characteristics. Parasites with small bud right body. They have not sexual pterygas. The anus is opened at the terminal part of the body. The cloaks are opened at the ¼ frontal part of the body. The adult females are larva-productive. They are parasites of the intestinal system. Trichinella spiralis (Trichina spiral) Owen, 1833. Eight species of Trichinella are now recognized, based on host (Kapel, C M O 2000; Krivokapich, S J; Pozio E and D S Zarlenga, 2005; Pozio E et al, 1992), but the most important for animals domestic are: T. spiralis found as parasitic diseases in humans, pigs, rodents, and many carnivorous animals, of Europe, Asia, North America, with specific pathologies in pigs. T. native parasite of wild carnivorous of Euro – Asiatic areas northern of parallel 40°. It is specific diseases of carnivorous and omnivorous animals. T.nelsoni found as a parasite of wilds carnivorous animals of Asiatic areas southern of parallel 40°. T. pseudospiralis is parasite of cats, rodents, and pigs. It is recognized from other species because of the adult forms have smaller dimensions and forms noncapsulated cists. T. spiralis is the cause of Trichinellosis, one of most important zoonosis all over the world. It is found worldwide in many carnivorous and omnivorous animals, insectivorous animals, rodents, wilds animals and humans (Pozio E and G Marucci 2003). It was found at 103 mammals. Occasionally may be found as a parasite of horses. Developmental traits of T. spiralis is that infested hosts initially are final hosts because of they host adults forms at their intestine, but later on, they are presented as an intermediate host, because of they host larval forms at their muscles. Today’s identification of samples to the species level and genotyping are based primarily upon molecular means (Pozio, E., and G. Marucci. 2003).Keywords: trichinosis, Trichinella larvae, tropism, trichinelloscopic examination, trypsine techniques, xenodiagnostic experiments, etc

Investigation on Trichinellosis of pigs in Saranda District

Trichinellosis caused by the Nematode Trichinella is a zoonotic disease which occurs worldwide and affects the broad range of different species including mammals, birds, and amphibians. Trichinella spiralis is found in production animals (pigs, horses) in temperate climate zones and can also be found in animals in close contact with these production animals (e.g. dogs, cats, rats). The PrioCHECK® Trichinella Ab is a reliable and fast diagnostic test for detection of antibodies against Trichinella in porcine serum and meat juice samples and can be used for monitoring and surveillance purposes.Keywords: Trichinella, pig, diagnosis, ELISA, Saranda

Investigation on Trichinellosis of pigs in Saranda District

Trichinellosis caused by the Nematode Trichinella is a zoonotic disease which occurs worldwide and affects the broad range of different species including mammals, birds, and amphibians. Trichinella spiralis is found in production animals (pigs, horses) in temperate climate zones and can also be found in animals in close contact with these production animals (e.g. dogs, cats, rats). The PrioCHECK® Trichinella Ab is a reliable and fast diagnostic test for detection of antibodies against Trichinella in porcine serum and meat juice samples and can be used for monitoring and surveillance purposes

Trichinelosis in Animals

Trichinellosis (also trichinosis) in animals is caused by nematodes (roundworms) of the family Trichinellidae Ward, 1970. Family characteristics. Parasites with small bud right body. They have not sexual pterygas. The anus is opened at the terminal part of the body. The cloaks are opened at the ¼ frontal part of the body. The adult females are larva-productive. They are parasites of the intestinal system. Trichinella spiralis (Trichina spiral) Owen, 1833. Eight species of Trichinella are now recognized, based on host (Kapel, C M O 2000; Krivokapich, S J; Pozio E and D S Zarlenga, 2005; Pozio E et al, 1992), but the most important for animals domestic are: T. spiralis found as parasitic diseases in humans, pigs, rodents, and many carnivorous animals, of Europe, Asia, North America, with specific pathologies in pigs. T. native parasite of wild carnivorous of Euro – Asiatic areas northern of parallel 40°. It is specific diseases of carnivorous and omnivorous animals. T.nelsoni found as a parasite of wilds carnivorous animals of Asiatic areas southern of parallel 40°. T. pseudospiralis is parasite of cats, rodents, and pigs. It is recognized from other species because of the adult forms have smaller dimensions and forms noncapsulated cists. T. spiralis is the cause of Trichinellosis, one of most important zoonosis all over the world. It is found worldwide in many carnivorous and omnivorous animals, insectivorous animals, rodents, wilds animals and humans (Pozio E and G Marucci 2003). It was found at 103 mammals. Occasionally may be found as a parasite of horses. Developmental traits of T. spiralis is that infested hosts initially are final hosts because of they host adults forms at their intestine, but later on, they are presented as an intermediate host, because of they host larval forms at their muscles. Today’s identification of samples to the species level and genotyping are based primarily upon molecular means (Pozio, E., and G. Marucci. 2003)

Evaluation of west nile virus diagnostic capacities in veterinary laboratories of the mediterranean and black sea regions

The increasing incidence of West Nile virus (WNV) in the Euro-Mediterranean area warrants the implementation of effective surveillance programs in animals. A crucial step in the fight against the disease is the evaluation of the capacity of the veterinary labs to accurately detect the infection in animal populations. In this context, the animal virology network of the MediLabSecure project organized an external quality assessment (EQA) to evaluate the WNV molecular and serological diagnostic capacities of beneficiary veterinary labs. Laboratories from 17 Mediterranean and Black Sea countries participated. The results of the triplex real time RT-PCR for simultaneous detection and differentiation of WNV lineage 1 (L1), lineage 2 (L2) and Usutu virus (USUV) were highly satisfactory, especially for L1 and L2, with detection rates of 97.9% and 100%, respectively. For USUV, 75% of the labs reported correct results. More limitations were observed for the generic detection of flaviviruses using conventional reverse-transcription polymerase chain reaction (RT-PCR), since only 46.1% reported correct results in the whole panel. As regards the serological panel, the results were excellent for the generic detection of WNV antibodies. More variability was observed for the specific detection of IgM antibodies with a higher percentage of incorrect results mainly in samples with low titers. This EQA provides a good overview of the WNV (and USUV) diagnostic performance of the involved veterinary labs and demonstrates that the implemented training program was successful in upgrading their diagnostic capacities

External quality assessment of Rift Valley fever diagnosis in 17 veterinary laboratories of the Mediterranean and Black Sea regions

Rift Valley fever (RVF) is an arboviral zoonosis that primarily affects ruminants but can also cause illness in humans. The increasing impact of RVF in Africa and Middle East and the risk of expansion to other areas such as Europe, where competent mosquitos are already established, require the implementation of efficient surveillance programs in animal populations. For that, it is pivotal to regularly assess the performance of existing diagnostic tests and to evaluate the capacity of veterinary labs of endemic and non-endemic countries to detect the infection in an accurate and timely manner. In this context, the animal virology network of the MediLabSecure project organized between October 2016 and March 2017 an external quality assessment (EQA) to evaluate the RVF diagnostic capacities of beneficiary veterinary labs. This EQA was conceived as the last step of a training curriculum that included 2 diagnostic workshops that were organized by INIA-CISA (Spain) in 2015 and 2016. Seventeen veterinary diagnostic labs from 17 countries in the Mediterranean and Black Sea regions participated in this EQA. The exercise consisted of two panels of samples for molecular and serological detection of the virus. The laboratories were also provided with positive controls and all the kits and reagents necessary to perform the recommended diagnostic techniques. All the labs were able to apply the different protocols and to provide the results on time. The performance was good in the molecular panel with 70.6% of participants reporting 100% correct results, and excellent in the serological panel with 100% correct results reported by 94.1% of the labs. This EQA provided a good overview of the RVFV diagnostic capacities of the involved labs and demonstrated that most of them were able to correctly identify the virus genome and antibodies in different animal samples