Single Chain Force Spectroscopy: Sequence Dependence

We study the elastic properties of a single A/B copolymer chain with a specific sequence. We predict a rich structure in the force extension relations which can be addressed to the sequence. The variational method is introduced to probe local minima on the path of stretching and releasing. At given force, we find multiple configurations which are separated by energy barriers. A collapsed globular configuration consists of several domains which unravel cooperatively. Upon stretching, unfolding path shows stepwise pattern corresponding to the unfolding of each domain. While releasing, several cores can be created simultaneously in the middle of the chain resulting in a different path of collapse.Comment: 6 pages 3 figure

Angoricity and compactivity describe the jamming transition in soft particulate matter

The application of concepts from equilibrium statistical mechanics to out-of-equilibrium systems has a long history of describing diverse systems ranging from glasses to granular materials. For dissipative jammed systems-- particulate grains or droplets-- a key concept is to replace the energy ensemble describing conservative systems by the volume-stress ensemble. Here, we test the applicability of the volume-stress ensemble to describe the jamming transition by comparing the jammed configurations obtained by dynamics with those averaged over the ensemble as a probe of ergodicity. Agreement between both methods suggests the idea of "thermalization" at a given angoricity and compactivity. We elucidate the thermodynamic order of the jamming transition by showing the absence of critical fluctuations in static observables like pressure and volume. The approach allows to calculate observables such as the entropy, volume, pressure, coordination number and distribution of forces to characterize the scaling laws near the jamming transition from a statistical mechanics viewpoint.Comment: 27 pages, 13 figure

The size and shape of the Milky Way disc and halo from M-type brown dwarfs in the BoRG survey.

We have identified 274 M-type brown dwarfs in the Hubble Space Telescope\u27s Wide Field Camera 3 pure parallel fields from the Brightest of Reionizing Galaxies (BoRG) survey for high-redshift galaxies. These are near-infrared observations with multiple lines of sight out of our Milky Way. Using these observed M-type brown dwarfs, we fitted a Galactic disc and halo model with a Markov chain Monte Carlo analysis. This model worked best with the scalelength of the disc fixed at h = 2.6 kpc. For the scaleheight of the disc, we found z0=0.29+0.02−0.019 z0=0.29−0.019+0.02 kpc and for the central number density, ρ0=0.29+0.20−0.13 ρ0=0.29−0.13+0.20 # pc−3. For the halo, we derived a flattening parameter κ = 0.45 ± 0.04 and a power-law index p = 2.4 ± 0.07. We found the fraction of M-type brown dwarfs in the local density that belong to the halo to be fh = 0.0075 +0.0025−0.0019 −0.0019+0.0025 . We found no correlation between subtype of M-dwarf and any model parameters. The total number of M-type brown dwarfs in the disc and halo was determined to be 58.2+9.81−6.70×109 58.2−6.70+9.81×109 . We found an upper limit for the fraction of M-type brown dwarfs in the halo of 7 +5−4 −4+5  per cent. The upper limit for the total Galactic disc mass in M-dwarfs is 4.34+0.73−0.5×109 4.34−0.5+0.73×109 M⊙, assuming all M-type brown dwarfs have a mass of 80 MJ

Identification and characterisation of tomato torrado virus, a new plant picorna-like virus from tomato

A new virus was isolated from tomato plants from the Murcia region in Spain which showed symptoms of ‘torrado disease’ very distinct necrotic, almost burn-like symptoms on leaves of infected plants. The virus particles are isometric with a diameter of approximately 28 nm. The viral genome consists of two (+)ssRNA molecules of 7793 (RNA1) and 5389 nts (RNA2). RNA1 contains one open reading frame (ORF) encoding a predicted polyprotein of 241 kDa that shows conserved regions with motifs typical for a protease-cofactor, a helicase, a protease and an RNA-dependent RNA polymerase. RNA2 contains two, partially overlapping ORFs potentially encoding proteins of 20 and 134 kDa. These viral RNAs are encapsidated by three proteins with estimated sizes of 35, 26 and 23 kDa. Direct protein sequencing mapped these coat proteins to ORF2 on RNA2. Phylogenetic analyses of nucleotide and derived amino acid sequences showed that the virus is related to but distinct from viruses belonging to the genera Sequivirus, Sadwavirus and Cheravirus. This new virus, for which the name tomato torrado virus is proposed, most likely represents a member of a new plant virus genus

Enantioselective adsorption of ibuprofen and lysine in metal-organic frameworks

This study reveals the efficient enantiomeric separation of bioactive molecules in the liquid phase. Chiral structure HMOF-1 separates racemic mixtures whereas heteroselectivity is observed for scalemic mixtures of ibuprofen using non-chiral MIL-47 and MIL-53. Lysine enantiomers are only separated by HMOF-1. These separations are controlled by the tight confinement of the molecules

Analysis of reflex modulation with a biologically realistic neural network

In this study, a neuromusculoskeletal model was built to give insight into the mechanisms behind the modulation of reflexive feedback strength as experimentally identified in the human shoulder joint. The model is an integration of a biologically realistic neural network consisting of motoneurons and interneurons, modeling 12 populations of spinal neurons, and a one degree-of-freedom musculoskeletal model, including proprioceptors. The model could mimic the findings of human postural experiments, using presynaptic inhibition of the Ia afferents to modulate the feedback gains. In a pathological case, disabling one specific neural connection between the inhibitory interneurons and the motoneurons could mimic the experimental findings in complex regional pain syndrome patients. It is concluded that the model is a valuable tool to gain insight into the spinal contributions to human motor control. Applications lay in the fields of human motor control and neurological disorders, where hypotheses on motor dysfunction can be tested, like spasticity, clonus, and tremor

The intermuscular 3–7 Hz drive is not affected by distal proprioceptive input in myoclonus-dystonia

In dystonia, both sensory malfunctioning and an abnormal intermuscular low-frequency drive of 3–7 Hz have been found, although cause and effect are unknown. It is hypothesized that sensory processing is primarily disturbed and induces this drive. Accordingly, experimenter-controlled sensory input should be able to influence the frequency of the drive. In six genetically confirmed myoclonus-dystonia (MD) patients and six matched controls, the low-frequency drive was studied with intermuscular coherence analysis. External perturbations were applied mechanically to the wrist joint in small frequency bands (0–4, 4–8 and 8–12 Hz; ‘angle protocol) and at single frequencies (1, 5, 7 and 9 Hz; ‘torque’ protocol). The low-frequency drive was found in the neck muscles of 4 MD patients. In these patients, its frequency did not shift due to the perturbation. In the torque protocol, the externally applied frequencies could be detected in all controls and in the two patients without the common drive. The common low-frequency drive was not be affected by external perturbations in MD patients. Furthermore, the torque protocol did not induce intermuscular coherences at the applied frequencies in these patients, as was the case in healthy controls and in patients without the drive. This suggests that the dystonic 3–7 Hz drive is caused by a sensory-independent motor drive and sensory malfunctioning in MD might rather be a consequence than a cause of dystonia