Biocontrol of weedy Sporobolus grasses in Australia using fungal pathogens

In Australia there are five weedy Sporobolus grass (WSG) species that heavily impact agricultural industries and native biodiversity. WSG have been the subject of several efforts to find host-specific pathogens with potential for classical and inundative biocontrol. Most of these studies are only discussed in unpublished reports or theses, so in this paper we synthesise the available peer-reviewed and ‘grey’ literature that discuss classical, augmentative and inundative biocontrol of WSG in Australia using fungal pathogens. We consider the hundreds of fungal pathogens previously isolated from Sporobolus hosts on an international and national scale. Of the pathogens investigated for WSG biocontrol previously, the only promising classical biocontrol agent was a smut fungus (Ustilago sporoboli-indici) from South Africa that is now present in Queensland and New South Wales, Australia. Its method of introduction to Australia is unknown. We hence discuss the history and potential for augmentative biocontrol of WSG using U. sporoboli-indici. Next, we summarise inundative biocontrol efforts. Several ascomycetes isolated from Australian WSG populations have been tested in this regard, including species of Nigrospora, Fusarium, Curvularia, Microdochium, Pestalotiopsis, and Neopestalotiopsis. However, a lack of host-specificity or efficacy subsequently precluded their further development, and potential improvements on those inundative biocontrol studies are discussed. Finally, we discuss a collection of endemic fungal taxa isolated from diseased Sporobolus in Australia, which are currently undergoing virulence, pathogenicity, and host-specificity screening as potential inundative biocontrol agents for WSG. Our intention is that the lessons learned from previous studies and summarised herein, will support ongoing development of WSG biocontrol agents in Australia, and more broadly, weed biocontrol using plant pathogens anywhere in the world

Chemical control of harungana (Harungana madagascariensis) shrubs in Queensland

Harungana (Harungana madagascarien�sis Lam. ex Poir), an aggressive exotic pioneering tree species, is currently invading the Wet Tropics World Heritage areas of north Queensland. A chemical trial involving ten herbicides was undertaken to identify effective chemicals to control harungana. For each herbicide, high volume foliar applications of two concentrations were applied. Of the ten herbicides tested, metsulfuron methyl, dicamba, imazapyr and glyphosate produced 100% mortality, at the rates applied, and the higher rate of fluroxypyr and 2,4-D/picloram killed greater than 95% of the treated plants. Metsulfuron and dicamba were the most target specific, while imazapyr, fluroxypyr, 2,4-D/ picloram and glyphosate had greater impact on non-target, native flora

Geographic range extension of Ustilago sporoboli-indici on Sporobolus natalensis in Australia

Field surveys during 2021–2023 found that the leaf smut fungus Ustilago sporoboli-indici was widespread on Sporobolus natalensis from northern New South Wales to northern Queensland. The geographic range of Ustilago sporoboli-indici has extended to 1500 km following the first Australian record of U. sporoboli-indici in south-east Queensland in 2017. Ustilago sporoboli-indici was not observed on other cohabitating species of Sporobolus

Monitoring Striga asiatica (Orobanchaceae) seedbank for eradication success

To enable evaluation of eradication efforts for the parasitic plant Striga asiatica (L.) Kuntze, seed sachets were buried in perforated PVC canisters at 25 sites across the infested area in July 2016. The canister sites were selected based on the proximity to previous S. asiatica detections and cover the range of soil types and topographies associated within the eradication program treatment area. At each site the canisters received the same eradication treatments that were applied to the surrounding area. Site characteristics necessitated different combinations of false host (soybean), true host (corn), fumigant (dazomet), stimulant (ethylene) and herbicide treatments, to achieve a rapid decline in the soil seed bank. The viability of retrieved seeds shows an accelerated decline (92.5 % to 6.5 % in four years) across all sites in response to the treatments irrespective of treatment combination used. The viability of seeds was found to vary across sites, burial depth and time. The information collected has provided valuable data for the eradication of S. asiatica in Australia and for evaluating the timing for release of paddocks from active treatment

Chemical Management of Senecio madagascariensis (Fireweed)

Fireweed (Senecio madagascariensis Poir.) is a herbaceous weed-producing pyrrolizidine alkaloid that is poisonous to livestock. To investigate the efficacy of chemical management on fireweed and its soil seed bank density, a field experiment was conducted in Beechmont, Queensland, in 2018 within a pasture community. A total of four herbicides (bromoxynil, fluroxypyr/aminopyralid, metsulfuron-methyl and triclopyr/picloram/aminopyralid) were applied either singularly or repeated after 3 months to a mix-aged population of fireweed. The initial fireweed plant density at the field site was high (10 to 18 plants m−2). However, after the first herbicide application, the fireweed plant density declined significantly (to ca. 0 to 4 plants m−2), with further reductions following the second treatment. Prior to herbicide application, fireweed seeds in both the upper (0 to 2 cm) and lower (2 to 10 cm) soil seed bank layers averaged 8804 and 3593 seeds m−2, respectively. Post-herbicide application, the seed density was significantly reduced in both the upper (970 seeds m−2) and lower (689 seeds m−2) seed bank layers. Based on the prevailing environmental conditions and nil grazing strategy of the current study, a single application of either fluroxypyr/aminopyralid, metsulfuron-methyl or triclopyr/picloram/aminopyralid would be sufficient to achieve effective control, whilst a second follow-up application is required with bromoxynil

Effect of microwave radiation on seed mortality of rubber vine (Cryptostegia grandiflora R.Br.), parthenium (Parthenium hysterophorous L.) and bellyache bush (Jatropha gossypiifolia L.)

A trial was undertaken to evaluate the effect of microwaves on seed mortality of three weed species. Seeds of rubber vine (Cryptostegia grandiflora R.Br.), parthenium (Parthenium hysterophorous L.) and bellyache bush (Jatropha gossypiifolia L.) were buried at six depths (0, 2.5, 5, 10, 20 and 40 cm) in coarse sand maintained at one of two moisture levels, oven dry or wet (field capacity), and then subjected to one of five microwave radiation durations of (0, 2, 4, 8 and 16 min). Significant interactions between soil moisture level, microwave radiation duration, seed burial depth and species were detected for mortality of seeds of all three species. Maximum seed mortality of rubber vine (88%), parthenium (67%) and bellyache bush (94%) occurred in wet soil irradiated for 16 min. Maximum seed mortality of rubber vine and bellyache bush seeds occurred in seeds buried at 2.5 cm depth whereas that of parthenium occurred in seeds buried at 10 cm depth. Maximum soil temperatures of 114.1 and 87.5°C in dry and wet soil respectively occurred at 2.5 cm depth following 16 min irradiation. Irrespective of the greater soil temperatures recorded in dry soil, irradiating seeds in wet soil generally increased seed mortality 2.9-fold compared with dry soil. Moisture content of wet soil averaged 5.7% compared with 0.1% for dry soil. Results suggest that microwave radiation has the potential to kill seeds located in the soil seed bank. However, many factors, including weed species susceptibility, determine the effectiveness of microwave radiation on buried seeds. Microwave radiation may be an alternative to conventional methods at rapidly depleting soil seed banks in the field, particularly in relatively wet soils that contain long lived weed seeds

Foliar herbicide control of sticky florestina (Florestina tripteris DC.)

Sticky florestina (Florestina tripteris DC.) is an annual exotic weed that has become naturalised near the townships of Tambo and Barcaldine in central western Queensland, Australia. Three experiments conducted near Barcaldine identified foliar herbicides effective in killing sticky florestina plants and in providing residual activity to reduce recruitment from the soil seed bank. An initial chemical screening experiment evaluated the efficacy of 28 herbicide treatments. The most promising herbicides were then further evaluated in two response-rate experiments. Overall, 2,4-D/picloram, aminopyralid/fluroxypyr, clopyralid, metsulfuron-methyl and triclopyr/picloram proved to be the most effective selective herbicides. Two of these, metsulfuron-methyl at 18 g active ingredient (a.i) ha–1 and 2,4-D + picloram at 900 g a.i. ha–1 + 225 g a.i. ha–1 have now been included in a minor use permit (PER11920) with the Australian Pesticides and Veterinary Medicines Authority (APVMA) for the control of sticky florestina in pasture, stock route, roadside and non-crop situations using both spot and boom-spray applications (APVMA 2010). The permit also allows the use of 2,4-D amine for the control of seedlings only

The diversity of microfungi associated with grasses in the Sporobolus indicus complex in Queensland, Australia

There are five closely related Sporobolus species, collectively known as weedy Sporobolus grasses (WSG) or the rat’s tail grasses. They are fast growing, highly competitive, unpalatable weeds of pastures, roadsides and woodlands. An effective biological control agent would be a welcomed alternative to successive herbicide application and manual removal methods. This study describes the initial exploratory phase of isolating and identifying native Australian microfungi associated with WSG, prior to evaluating their efficacy as inundative biological control agents. Accurate species-level identification of plant-pathogenic microfungi associated with WSG is an essential first step in the evaluation and prioritisation of pathogenicity bioassays. Starting with more than 79 unique fungal morphotypes isolated from diseased Sporobolus grasses in Queensland, Australia, we employed multi-locus phylogenetic analyses to classify these isolates into 54 fungal taxa. These taxa belong to 22 Ascomycete families (12 orders), of which the majority fall within the Pleosporales (>24 taxa in 7 families). In the next phase of the study, the putative species identities of these taxa will allow us to prioritise those which are likely to be pathogenic based on existing literature and their known ecological roles. This study represents the first step in a systematic, high-throughput approach to finding potential plant pathogenic biological control agents

Weed leucaena and its significance, implications and control

Leucaena (Leucaena leucocephala) is widely recognized in many countries as a commercially valuable plant, particularly when used as a nutritious fodder in subtropical and tropical regions. However, it is also considered an environmental weed in some countries due to its ability to form dense infestations in disturbed areas, where it is not proactively managed or grazed. These different perspectives have made leucaena a contentious species. Ideally, landholders and relevant jurisdictions in charge of invasive species need to work together to minimize its spread as a weed and manage existing infestations. To date, the response has been varied, ranging from no action through to some jurisdictions formally recognizing leucaena as an environmental weed within relevant legislation and applying requirements to minimize its impact. Between these extremes, there are initiatives such as an industry Code of Practice (i.e. The Leucaena Network in Australia), recommending that those growing leucaena adhere to certain principles and practices to minimize the risk of spread from their operations. The biology of weed leucaena (e.g. large seed production, relatively long-lived seed banks) and the situations in which it spreads (e.g. roadsides and riparian systems) pose management challenges to landholders and relevant jurisdictions. Adaptive management and experimental research are necessary to identify effective control strategies for a range of situations. © 2019, Centro Internacional de Agricultura Tropical (CIAT)