37 research outputs found
Analysis and quantification of diagnostic serum markers and protein signatures for Gaucher disease
FWN – Publicaties zonder aanstelling Universiteit Leide
Comparison of spherically and irregularly shaped stationary phase packings in microcolumn liquid chromatography
Spherically and irregularly shaped reversed phase packings were used to slurry pack capillary fused silica columns. The selection of the packing solvents was based on the colloidal properties of the stationary phase particles and investigated by sedimentation experiments. The chromatographic performance of the microcolumns was measured with conventional parameters from plate and rate theories, and the column resistance parameter and separation impedance. Also studied was the time of analysis. The performance of spherical and irregular packings was comparable with a light preference for spherically shaped materials when time of analysis is concerned
Accuracy and Reproducibility in Quantification of Plasma Protein Concentrations by Mass Spectrometry without the Use of Isotopic Standards
Medical Biochemistr
The Hamaker and the Lifshitz approaches for the Van der Waals interaction between particles of composite materials dispersed in a medium
Based on the superpositional principle of the classical Hamaker–De Boer approach to the Van der Waals interaction, an equation for the interaction energy between two identical composite particles in a medium has been developed. The particles are supposed to be composed of an arbitrary number of components that are intimately mixed to avoid appreciable scattering of interacting EM waves. A discussion is presented on how intimately the components have to be mixed for the theory to be applicable. As an example, the main theoretical result is applied to porous polystyrene particles in water, while the pores are not filled with water (‘non-wetting surface’). Numerical results for composite particles are evaluated, using interaction parameters as obtained by various methods that are based on either the Hamaker–de Boer or the Lifshitz approach
The Hamaker and the Lifshitz approaches for the Van der Waals interaction between particles of composite materials dispersed in a medium
Based on the superpositional principle of the classical Hamaker–De Boer approach to the Van der Waals interaction, an equation for the interaction energy between two identical composite particles in a medium has been developed. The particles are supposed to be composed of an arbitrary number of components that are intimately mixed to avoid appreciable scattering of interacting EM waves. A discussion is presented on how intimately the components have to be mixed for the theory to be applicable. As an example, the main theoretical result is applied to porous polystyrene particles in water, while the pores are not filled with water (‘non-wetting surface’). Numerical results for composite particles are evaluated, using interaction parameters as obtained by various methods that are based on either the Hamaker–de Boer or the Lifshitz approach
Quantitative proteomics of rat and human pancreatic beta cells
Data set description: This data set is composed by label-free alternate-scanning LC-MS/MS proteomics analysis human and Wistar rat pancreatic islet endocrine cells.
The mass spectrometry data of the human and rat pancreatic beta cells and the resulting proteome search output from ProteinLynx GlobalSERVER (PLGS) have been deposited to the ProteomeXchange Consortium [1] via the PRIDE partner repository with the dataset identifiers PXD001539 (human) and PXD001816 (rat). From these mass spectrometry data, ‘relative molar amount units’ between cell types and across species were calculated.
Biological relevance: These data provide a quantitative view on the unfractionated proteomes of human and rat beta and alpha cells. It is likely biased towards the proteins with higher molar abundance, relating to core functional pathways, but also includes several proteins with an islet-enriched expression. The quality of the cell preps is state-of-the-art, and the label-free quantitation is both precise and accurate, allowing detailed quantitative analysis