Damparis – À la Vignotte

La municipalité de Damparis (Jura), commune située à environ 5 km au sud-ouest de Dole, projette la création d’un lotissement communal au lieu-dit « À la Vignotte ». Dans ce souci de la préservation et de l’étude du patrimoine enfoui, et compte tenu de la richesse archéologique de la région du Finage et plus particulièrement du territoire de la commune de Damparis, le Service régional de l’archéologie de Franche-Comté a décidé l’exécution d’un diagnostic archéologique préalable aux travaux de..

Monnières – Carrière du Mont-Roland

La société d’exploitation des carrières de Monnières (Jura), commune située à environ 2 km au nord-ouest de Dole, souhaite étendre sa carrière du Mont-Roland vers le nord. Le projet concerne les parcelles AC (8,23), un terrain actuellement en friche d’une superficie d’environ 3,7 ha. Dans le souci de la préservation et de l’étude du patrimoine enfoui, et compte tenu de la richesse archéologique de la région et plus particulièrement du Mont-Roland, le service régional de l’archéologie de Franc..

Saint-Aubin – La Corvée Séguine

La municipalité de Saint-Aubin, commune située à environ 13 km au sud-ouest de Dole, projette l’extension de son lotissement communal sur les parcelles ZE (64) au lieu-dit La Corvée Séguine. Dans le souci de la préservation et de l’étude du patrimoine enfoui, et compte tenu de la richesse archéologique de la région du Finage et plus particulièrement du territoire de la commune de Saint-Aubin, le service régional de l’archéologie de Franche-Comté a décidé l’exécution d’un diagnostic archéologi..

The Different Strategies Used by Salmonella to Invade Host Cells

Chapitre 17International audienc

Brief Report: Incubation Period Duration and Severity of Clinical Disease Following Severe Acute Respiratory Syndrome Coronavirus Infection

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Early immune response following Salmonella enterica subspecies enterica serovar Typhimurium infection in porcine jejunal gut loops

Salmonella enterica subspecies enterica serovar Typhimurium, commonly called S. Typhimurium, can cause intestinal infections in humans and various animal species such as swine. To analyze the host response to Salmonella infection in the pig we used an in vivo gut loop model, which allows the analysis of multiple immune responses within the same animal. Four jejunal gut-loops were each inoculated with 3×108 cfu of S. Typhimurium in 3 one-month-old piglets and mRNA expressions of various cytokines, chemokines, transcription factors, antimicrobial peptides, toll like and chemokine receptors were assessed by quantitative real-time PCR in the Peyer’s patch and the gut wall after 24 h. Several genes such as the newly cloned CCRL1/CCX-CKR were assessed for the first time in the pig at the mRNA level. Pro-inflammatory and T-helper type-1 (Th1) cytokine mRNA were expressed at higher levels in infected compared to non-infected control loops. Similarly, some B cell activation genes, NOD2 and toll like receptor 2 and 4 transcripts were more expressed in both tissues while TLR5 mRNA was down-regulated. Interestingly, CCL25 mRNA expression as well as the mRNA expressions of its receptors CCR9 and CCRL1 were decreased both in the Peyer’s patch and gut wall suggesting a potential Salmonella strategy to reduce lymphocyte homing to the intestine. In conclusion, these results provide insight into the porcine innate mucosal immune response to infection with entero-invasive microorganisms such as S. Typhimurium. In the future, this knowledge should help in the development of improved prophylactic and therapeutic approaches against porcine intestinal S. Typhimurium infections

Deciphering why Salmonella Gallinarum is less invasive in vitro than Salmonella Enteritidis

International audienceSalmonella Gallinarum and Salmonella Enteritidis are genetically closely related however associated with different pathologies. Several studies have suggested that S. Gallinarum is less invasive in vitro than S. Enteritidis. In this study we confirm that the S. Gallinarum strains tested were much less invasive than the S. Enteritidis strains tested in cells of avian or human origin. In addition, the S. Gallinarum T3SS-1-dependent ability to invade host cells was delayed by two to three hours compared to S. Enteritidis, indicating that T3SS-1-dependent entry is less efficient in S. Gallinarum than S. Enteritidis. This was neither due to a decreased transcription of T3SS-1 related genes when bacteria come into contact with cells, as transcription of hilA, invF and sipA was similar to that observed for S. Enteritidis, nor to a lack of functionality of the S. Gallinarum T3SS-1 apparatus as this apparatus was able to secrete and translocate effector proteins into host cells. In contrast, genome comparison of four S. Gallinarum and two S. Enteritidis strains revealed that all S. Gallinarum genomes displayed the same point mutations in each of the main T3SS-1 effector genes sipA, sopE, sopE2, sopD and sopA

Heterogeneity of Persistence of Salmonella enterica Serotype Senftenberg Strains Could Explain the Emergence of this Serotype in Poultry Flocks

Salmonella enterica serotype Senftenberg (S. Senftenberg) has recently become more frequent in poultry flocks. Moreover some strains have been implicated in severe clinical cases. To explain the causes of this emergence in farm animals, 134 S. Senftenberg isolates from hatcheries, poultry farms and human clinical cases were analyzed. Persistent and non-persistent strains were identified in chicks. The non-persistent strains disappeared from ceca a few weeks post inoculation. This lack of persistence could be related to the disappearance of this serotype from poultry farms in the past. In contrast, persistent S. Senftenberg strains induced an intestinal asymptomatic carrier state in chicks similar to S. Enteritidis, but a weaker systemic infection than S. Enteritidis in chicks and mice. An in vitro analysis showed that the low infectivity of S. Senftenberg is in part related to its low capacity to invade enterocytes and thus to translocate the intestinal barrier. The higher capacity of persistent than non-persistent strains to colonize and persist in the ceca of chickens could explain the increased persistence of S. Senftenberg in poultry flocks. This trait might thus present a human health risk as these bacteria could be present in animals before slaughter and during food processing