A role for Phospholipase D in Drosophila embryonic cellularization

BACKGROUND: Cellularization of the Drosophila embryo is an unusually synchronous form of cytokinesis in which polarized membrane extension proceeds in part through incorporation of new membrane via fusion of apically-translocated Golgi-derived vesicles. RESULTS: We describe here involvement of the signaling enzyme Phospholipase D (Pld) in regulation of this developmental step. Functional analysis using gene targeting revealed that cellularization is hindered by the loss of Pld, resulting frequently in early embryonic developmental arrest. Mechanistically, chronic Pld deficiency causes abnormal Golgi structure and secretory vesicle trafficking. CONCLUSION: Our results suggest that Pld functions to promote trafficking of Golgi-derived fusion-competent vesicles during cellularization

Ginkgo Biloba Extract Ameliorates Oxidative Phosphorylation Performance and Rescues Aβ-Induced Failure

Energy deficiency and mitochondrial failure have been recognized as a prominent, early event in Alzheimer's disease (AD). Recently, we demonstrated that chronic exposure to amyloid-beta (Abeta) in human neuroblastoma cells over-expressing human wild-type amyloid precursor protein (APP) resulted in (i) activity changes of complexes III and IV of the oxidative phosphorylation system (OXPHOS) and in (ii) a drop of ATP levels which may finally instigate loss of synapses and neuronal cell death in AD. Therefore, the aim of the present study was to investigate whether standardized Ginkgo biloba extract LI 1370 (GBE) is able to rescue Abeta-induced defects in energy metabolism

Nephropathic cystinosis : pathophysiology, adaptations and investigations towards a new rationale treatment

Nephropathic cystinosis is a multisystemic lysosomal storage disease due to genetic absence of functional cystinosin gene, which encodes the lysosomal cystine transporter. Suppressed lysosomal exodus leads to cystine accumulation then precipitation as crystals. The earliest clinical manifestation of cystinosis is a renal Fanconi syndrome which reflects global dysfunction of proximal tubular cells (PTCs). I first addressed early pathophysiological changes in PTCs of a cystinosin KO mouse model and identified apical dedifferentiation related to lysosome swelling, prior to crystals and atrophy. I next found that excision of megalin in cystinotic PTCs prevented cystine accumulation and protected kidney structure. This implies that endocytosis is the main source of cystine accumulation, which itself causes kidney damage. In a promising translational approach, I found that megalin inhibition by a simple dietary supplementation in lysine or arginine could slow down disease progression.(BIFA - Sciences biomédicales et pharmaceutiques) -- UCL, 201

BHS guidelines for the management of small lymphocytic lymphoma and chronic lymphocytic leukaemia, anno 2024

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Multi-output machine learning models for kinetic data evaluation : A Fischer–Tropsch synthesis case study

International audienc

Machine learning based interpretation of microkinetic data: a Fischer–Tropsch synthesis case study

International audienceA systematic approach for analysing kinetic data and identifying hidden trends using interpretation techniques in data science with the ANN

Hodgkin’s lymphoma: Belgina hematology Society guidelines in diagnosis, treatment and follow-up

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