114 research outputs found
Elevated levels of low molecular weight substances in the red cells of some mammalian species imply unsuspected antioxidant strategies
- Author
- Publication venue
- Department of Clinical Laboratory Sciences
- Publication date
- 01/01/2009
- Field of study
An earlier observation by E.H. Harley (supervisor of this thesis) of curious metabolic anomalies in the red cells of black rhinoceros, and in particular a high free tyrosine level, suggested that a range of unusual, but presumeably physiological, processes might be found in mammalian red blood cells. As a follow-up to this, low molecular weight metabolites were examined in a range of mammalian species, using HPLC-based methods to compare levels in red cells with plasma levels. A remarkable interspecies diversity in red cell HPLC profiles was observed, with the unprecedented accumulation of substances including tyrosine, tryptophan, urate, and urate riboside occuring within the red cells of some species. Whereas novel evolutionary adaptations may characterise most of these species-specific variations, the ability of red cells to produce urate is proposed to be an inducible feature common to the red cells of many, or possibly even all, mammalian species. A surprisingly high degree of intraspecies genetic heterogeneity was evident in tyrosine and urate levels within horse, and urate riboside levels within cow red cells. This was in contrast with the greater homogeneity seen in levels of these and other low molecular weight substances in red cells from the other species evaluated. The next phase of investigation addressed the potential function(s) of these soluble substances accumulating within the red cell, particularly relating to a role in antioxidant defense. Using in vitro antioxidant assays such as the 'oxygen radical absorbance' (ORAC) and 'ferrous ion oxidation-xylenol orange' (FOX) assays, results were obtained consistent with a role for these substances as endogenous red cell antioxidants against a variety of reactive species produced by pathophysiological processes in the body. The demonstration that haemoglobin is involved in facilitating some of this activity further substantiates the idea that the red cell may be playing a crucial role in maintaining circulatory redox balance, and hence protecting other tissues from oxidative damage. If indeed such low molecular weight substances contribute to systemic antioxidant activity in some mammalian species, then apart from the intrinsic interest of such unexpected biological phenomena, these findings could pave the way for a plethora of further investigations, geared towards potential clinical applications (eg. as biomarkers or therapeutic approaches) in human and/or veterinary conditions associated with oxidative stress
Induction of Granulysin and Perforin Cytolytic Mediator Expression in 10-Week-Old Infants Vaccinated with BCG at Birth
- Author
- Publication venue
- Hindawi Publishing Corporation
- Publication date
- 01/01/2011
- Field of study
Background. While vaccination at birth with Mycobacterium bovis Bacilli Calmette-Guérin (BCG) protects against severe childhood tuberculosis, there is no consensus as to which components of the BCG-induced immune response mediate this protection. However, granulysin and perforin, found in the granules of cytotoxic T lymphocytes and Natural Killer (NK) cells, can kill intracellular mycobacteria and are implicated in protection against Mycobacterium tuberculosis. Methods. We compared the cellular expression of granulysin and perforin cytolytic molecules in cord blood and peripheral blood from 10-week-old infants vaccinated at birth with either Japanese or Danish BCG, administered either intradermally or percutaneously. Results. In cord blood, only CD56+ NK cells expressed granulysin and perforin constitutively. These cytolytic mediators were upregulated in CD4+ and CD8+ cord blood cells by ex vivo stimulation with BCG but not with PPD. Following BCG vaccination of neonates, both BCG and PPD induced increased expression of granulysin and perforin by CD4+ and CD8+ T cells. There was no difference in expression of cytolytic molecules according to vaccination route or strain. Conclusions. Constitutive expression of perforin and granulysin by cord blood NK-cells likely provides innate immunity, while BCG vaccination-induced expression of these cytolytic mediators may contribute towards protection of the neonate against tuberculosis
Clinical Utility of a Commercial LAM-ELISA Assay for TB Diagnosis in HIV-Infected Patients Using Urine and Sputum Samples
- Author
- Publication venue
- Public Library of Science
- Publication date
- 01/01/2010
- Field of study
Background: The accurate diagnosis of TB in HIV-infected patients, particularly with advanced immunosuppression, is difficult. Recent studies indicate that a lipoarabinomannan (LAM) assay (Clearview-TB (R)-ELISA) may have some utility for the diagnosis of TB in HIV-infected patients; however, the precise subgroup that may benefit from this technology requires clarification. The utility of LAM in sputum samples has, hitherto, not been evaluated.Methods: LAM was measured in sputum and urine samples obtained from 500 consecutively recruited ambulant patients, with suspected TB, from 2 primary care clinics in South Africa. Culture positivity for M. tuberculosis was used as the reference standard for TB diagnosis.Results: Of 440 evaluable patients 120/387 (31%) were HIV-infected. Urine-LAM positivity was associated with HIV positivity (p = 0.007) and test sensitivity, although low, was significantly higher in HIV-infected compared to uninfected patients (21% versus 6%; p<0.001), and also in HIV-infected participants with a CD4 <200 versus <200 cells/mm(3) (37% versus 0%; p = 0.003). Urine-LAM remained highly specific in all 3 subgroups (95%-100%). 25% of smear-negative but culture-positive HIV-infected patients with a CD4 <200 cells/mm(3) were positive for urine-LAM. Sputum-LAM had good sensitivity (86%) but poor specificity (15%) likely due to test cross-reactivity with several mouth-residing organisms including actinomycetes and nocardia species.Conclusions: These preliminary data indicate that in a high burden primary care setting the diagnostic usefulness of urine-LAM is limited, as a rule-in test, to a specific patient subgroup i.e. smear-negative HIV-infected TB patients with a CD4 count <200 cells/mm(3), who would otherwise have required further investigation. However, even in this group sensitivity was modest. Future and adequately powered studies in a primary care setting should now specifically target patients with suspected TB who have advanced HIV infection
Risk factors for Coronavirus Disease 2019 (COVID-19) death in a population cohort study from the Western Cape Province, South Africa
- Author
- Adams K.
- Allen D.
- Allie T.
- Audley G.
- Bam J.L.
- Bateman K.
- Beckwith P.
- Bernon M.
- Blom D.
- Blumberg Lucille Hellen
- Boloko L.
- Botha J.
- Boulle Andrew
- Boutall A.
- Buddiga K.
- Burmeister S.
- Cairncross L.
- Calligaro G.
- Coccia C.
- Corin C.
- Cragg C.
- Dane P.
- Daroowala R.
- Dave J.A.
- Davids J.
- Davies Mary-Ann
- De Bruyn E.
- De Villiers M.
- Deetlefs M.
- Dlamini S.
- Du Toit T.
- Endres W.
- Europa T.
- Fieggan G.
- Figaji A.
- Frankenfeld P.
- Gatley E.
- Gina P.
- Govender E.
- Grobler R.
- Gule M.V.
- Hanekom C.
- Heekes A.
- Held M.
- Heynes A.
- Hlatswayo S.
- Hodkinson B.
- Holtzhausen J.
- Hoosain S.
- Hussey Hannah
- Isaacs W.
- Ismail Muzzammil
- Jacobs A.
- Kahn M.
- Kahn T.
- Khamajeet A.
- Khan J.
- Khan R.
- Khwitshana A.
- Knight L.
- Kooverjee S.
- Krogscheepers R.
- Kruger J.J.
- Kuhn S.,
- Lawrence C.
- Mahomed H.
- Marais F.
- Mathema Hlengani
- Matlapeng B.
- Morden Erna
- Mudaly V.
- Mutemaringa T.
- Muzarabani L.
- Paleker M.
- Phelanyane F.
- Pienaar D.
- Pienaar R.
- Rode C.
- Roodt F.
- Smit N.V.
- Smith M.
- Tembo Y.
- Tiffin N.
- Vermeulen A.
- Voget J.
- Vundle Ziyanda
- Zinyakatira N.
- Zweigenthal Virginia
- Publication venue
- 'Oxford University Press (OUP)'
- Publication date
- 01/10/2021
- Field of study
BACKGROUND. Risk factors for coronavirus disease 2019 (COVID-19) death in sub-Saharan Africa and the effects of human immunodeficiency
virus (HIV) and tuberculosis on COVID-19 outcomes are unknown.
METHODS. We conducted a population cohort study using linked data from adults attending public-sector health facilities in the
Western Cape, South Africa. We used Cox proportional hazards models, adjusted for age, sex, location, and comorbidities, to examine
the associations between HIV, tuberculosis, and COVID-19 death from 1 March to 9 June 2020 among (1) public-sector “active
patients” (≥1 visit in the 3 years before March 2020); (2) laboratory-diagnosed COVID-19 cases; and (3) hospitalized COVID-19
cases. We calculated the standardized mortality ratio (SMR) for COVID-19, comparing adults living with and without HIV using
modeled population estimates.
RESULTS. Among 3 460 932 patients (16% living with HIV), 22 308 were diagnosed with COVID-19, of whom 625 died. COVID-
19 death was associated with male sex, increasing age, diabetes, hypertension, and chronic kidney disease. HIV was associated with
COVID-19 mortality (adjusted hazard ratio [aHR], 2.14; 95% confidence interval [CI], 1.70–2.70), with similar risks across strata of
viral loads and immunosuppression. Current and previous diagnoses of tuberculosis were associated with COVID-19 death (aHR,
2.70 [95% CI, 1.81–4.04] and 1.51 [95% CI, 1.18–1.93], respectively). The SMR for COVID-19 death associated with HIV was 2.39
(95% CI, 1.96–2.86); population attributable fraction 8.5% (95% CI, 6.1–11.1).
CONCLUSIONS. While our findings may overestimate HIV- and tuberculosis-associated COVID-19 mortality risks due to residual
confounding, both living with HIV and having current tuberculosis were independently associated with increased COVID-19 mortality.
The associations between age, sex, and other comorbidities and COVID-19 mortality were similar to those in other settings.The Western Cape Provincial Health Data Centre from the Western Cape Department of Health, the US National Institutes for Health (grant numbers R01 HD0804, the Bill and Melinda Gates Foundation, the United States Agency for International Development and the Wellcome Trust.https://academic.oup.com/cid/am2023Veterinary Tropical Disease
Tropical Data: Approach and Methodology as Applied to Trachoma Prevalence Surveys
- Author
- Abdala Mariamo
- Abdi Hirpa M
- Abdou Amza
- Adamu Yilikal
- Al-Khatib Tawfik
- Alemayehu Addisu
- Alemayehu Wondu
- Apadinuwe Sue-Chen
- Awaca Naomie
- Awoussi Marcel S
- Baayendag Gilbert
- Badiane Mouctar Dieng
- Bailey Robin L
- Bakhtiari Ana
- Batcho Wilfrid
- Bay Zulficar
- Beido Nassirou
- Bejiga Michael Dejene
- Bella Assumpta
- Bol Yak Yak
- Bougouma Clarisse
- Boyd Sarah
- Brady Christopher J
- Bucumi Victor
- Burgert-Brucker Clara R
- Butcher Robert
- Cakacaka Risiate
- Cama Anaseini
- Camara Mamoudou
- Cassama Eunice
- Chaora Shorai Grace
- Chebbi Amel Chenaoui
- Chisambi Alvin Blessings
- Chu Brian
- Conteh Abdulai
- Coulibaly Sidi Mohamed
- Courtright Paul
- Dalmar Abdi
- Dat Tran Minh
- Davids Thully
- de Fátima Costa Lopes Maria
- Djaker Mohamed El Amine
- Dodson Sarity
- Downs Philip
- Dézoumbé Djore
- Eckman Stephanie
- Elmezoghi Mourad
- Elshafie Bilghis Elkhair
- Elvis Ange Aba
- Emerson Paul
- Epée Emilienne Ee
- Faktaufon Daniel
- Fall Mawo
- Fassinou Aréty
- Fleming Fiona
- Flueckiger Rebecca
- Gamael Koizan Kadjo
- Garae Mackline
- Garap Jambi
- Gass Katie
- Gebru Genet
- Gichangi Michael M
- Giorgi Emanuele
- Gower Emily W
- Goépogui André
- Gómez Forero Diana Paola
- Gómez Daniela Vaz Ferreira
- Harding-Esch Emma M
- Harte Anna
- Henry Rob
- Honorio-Morales Harvy Alberto
- Hooper Pamela J
- Ilako Dunera R
- Issifou Amadou Alfa Bio
- Jimenez Cristina
- Jones Ellen
- Kabona George
- Kabore Martin
- Kadri Boubacar
- Kalua Khumbo
- Kanyi Sarjo Kebba
- Kebede Fikreab
- Kebede Shambel
- Keenan Jeremy D
- Kello Amir B
- Khan Asad Aslam
- Khelifi Houria
- Kilangalanga Janvier
- Kim Sung Hye
- Ko Robert
- Lewallen Susan
- Lietman Thomas
- Logora Makoy Samuel Yibi
- Lopez Yuri A
- M'Po Nerkoua
- MacArthur Chad
- Macleod Colin
- Makangila Felix
- Mariko Brehima
- Martin Diana L
- Masika Michael
- Massae Patrick
- Massangaie Marilia
- Matendechero Hadley S
- Mathewos Tsedeke
- McCullagh Siobhain
- Meite Aboulaye
- Mendes Elsa Palma
- Millar Tom
- Miller Hollman
- Minnih Abdellahi
- Mishra Sailesh Kumar
- Molefi Tuduetso
- Mosher Aryc
- Mpyet Caleb
- Mugume Francis
- Mukwiza Robson
- Mwale Consity
- Mwatha Stephen
- Mwingira Upendo
- Nash Scott D
- Nassa Christophe
- Negussu Nebiyu
- Ngondi Jeremiah
- Nieba Cece
- Noah Noah Jean Claude
- Nwosu Christian O
- Olobio Nicholas
- Opon Rapheal
- Pavluck Alexandre
- Phiri Isaac
- Rainima-Qaniuci Merelesita
- Renneker Kristen K
- Rotondo Lisa
- Saboyá-Díaz Martha Idalí
- Sakho Fatoumata
- Sanha Salimato
- Sarah Virginia
- Sarr Boubacar
- Seife Fikre
- Serrano Chavez Gloria Marina
- Shah Salam Ahmad
- Sharma Shekhar
- Sissoko Mactar
- Sitoe Henis Mior
- Sokana Oliver
- Solomon Anthony W
- Szwarcwald Celia L
- Tadesse Fentahun
- Taleo Fasiah
- Talero Sandra Liliana
- Tarfani Youcef
- Tefera Amsayaw
- Tekeraoi Rabebe
- Tesfazion Andeberhan
- Traina Abubaker
- Traoré Lamine
- Trujillo-Trujillo Julián
- Tukahebwa Edridah M
- Vashist Praveen
- Wanyama Ernest B
- Warusavithana Supriya DP
- Watitu Titus K
- West Sheila
- Willis Rebecca
- Win Ye
- Woods Geordie
- Yajima Aya
- Yaya Georges
- Zecarias Alem
- Zewengiel Solomon
- Zoumanigui Akoi
- Publication venue
- Taylor and Francis Group
- Publication date
- 12/12/2023
- Field of study
PURPOSE: Population-based prevalence surveys are essential for decision-making on interventions to achieve trachoma elimination as a public health problem. This paper outlines the methodologies of Tropical Data, which supports work to undertake those surveys. METHODS: Tropical Data is a consortium of partners that supports health ministries worldwide to conduct globally standardised prevalence surveys that conform to World Health Organization recommendations. Founding principles are health ministry ownership, partnership and collaboration, and quality assurance and quality control at every step of the survey process. Support covers survey planning, survey design, training, electronic data collection and fieldwork, and data management, analysis and dissemination. Methods are adapted to meet local context and needs. Customisations, operational research and integration of other diseases into routine trachoma surveys have also been supported. RESULTS: Between 29th February 2016 and 24th April 2023, 3373 trachoma surveys across 50 countries have been supported, resulting in 10,818,502 people being examined for trachoma. CONCLUSION: This health ministry-led, standardised approach, with support from the start to the end of the survey process, has helped all trachoma elimination stakeholders to know where interventions are needed, where interventions can be stopped, and when elimination as a public health problem has been achieved. Flexibility to meet specific country contexts, adaptation to changes in global guidance and adjustments in response to user feedback have facilitated innovation in evidence-based methodologies, and supported health ministries to strive for global disease control targets
Risk factors for Coronavirus disease 2019 (Covid-19) death in a population cohort study from the Western Cape province, South Africa
- Author
- A Sayed
- Abdurasiet Mowlana
- Abraham J Viljoen
- Adam Boutall
- Alana Heynes
- Alda Vermeulen
- Alexa Heekes
- Alicia Khwitshana
- Alistair Broadhurst
- Allison Moore
- Andre Nortje
- Andrew Boulle
- Annie Lombard
- Anthony Figaji
- Arifa Parker
- Arvin Khamajeet
- Ashely Jacobs
- Ashley Moodley
- Ayanda Mnguni
- Babalwa Ndebele
- Barbra Vermooten
- Boitumelo Mashigo
- Boitumelo Matlapeng
- Brian Allwood
- Bridget Hodkinson
- Bulewa Mbena
- Candice Van Koningsbruggen
- Cari Stek
- Carol Cragg
- Caroline Neumuller
- Catherine Rode
- Cecilia Coccia
- Chadwin Corin
- Chané Paulsen
- Charlene Lawrence
- Che Daniel
- Cheryl Cohen
- Chima Ofoegbu
- Chizama Mnqwazi
- Christoff Hanekom
- Christoffel Botha
- Clint Hendrikse
- Clint Johannes
- Coenraad F N Koegelenberg
- David Marais
- David Pienaar
- De Vries Basson
- Deborah Maughan
- Derick Allen
- Desiree Moodley
- Dion Levin
- Dirk Blom
- Dirkie Claasen
- Dusica Stapar
- Eddy Zitha
- Eduard Langenegger
- Eize Louw
- Elbe Zwanepoel
- Elizabeth Gatley
- Elsa De Bruyn
- Elsabe Smith
- Elzanne Olivier
- Emma Kalk
- Erna Morden
- Ernst Möller
- Ethel Linden-Mars
- Evashan Govender
- Florence Phelanyane
- Francois Maritz
- Francois Roodt
- Frederick Marais
- Funeka Nyengane
- Gabriela Patten
- Gary Maartens
- Gerrit Viljoen
- Gideon Titus
- Gordon Audley
- Gordon Robertson
- Graeme Meintjes
- Graham Fieggan
- Gregory Calligaro
- Hannah Hussey
- Hans Prozesky
- Hassan Mahomed
- Heather Jaspan
- Helen Schneider
- Hennie Sekhukhune
- Henri Pickard
- Hlengani Mathema
- Jacque Le Roux
- Jacqueline Voget
- Jacques Rood
- James Porter
- James Van Duuren
- Jamy-Lee Bam
- Jantjie J Taljaard
- Jean Botha
- Jean Jacque Kruger
- Jeanette Holtzhausen
- Jody Davids
- Joel A Dave
- John Lazarus
- Jon Ambler
- Jonathan Euvrard
- Jonathan Lucas
- Jonathan Naude
- Jonno Vogel
- Jonny Peter
- Joubin Khan
- Juanita Arendse
- Juanita McClaughlin
- Julius Rozmiarek
- Kasturi Buddiga
- Kathleen Bateman
- Kathleen Murie
- Katrin Stuve
- Katya Evans
- Kent Pluke
- Kerrigan McCarthy
- Kerrin Begg
- Kevin Adams
- Kevin Lee
- Kim Anderson
- Kim Laubscher
- Kobie Erasmus
- Lauren Knight
- Le Roux van der Merwe
- Lee Alan Wallis
- Leigh Johnson
- Leilah Najjaar
- Lenny Naidoo
- Liesel Petersen
- Lilian Dudley
- Linda Aldum
- Linda Boloko
- Lisa Boerlage
- Lizanne Victor
- Lucille Blumberg
- Luckmore Muzarabani
- Luthando Vazi
- Luyanda Mbanga
- Lydia Cairncross
- Lynn Morris
- Manqoba Vusumuzi Gule
- Marc Bernon
- Marc Mendelson
- Marc Nortje
- Marie du Preez
- Mariette Smith
- Marije van Schalkwyk
- Mariska Kotze
- Mark Wates
- Martin de Man
- Martin De Villiers
- Mary-Ann Davies
- Masudah Paleker
- Matthias Scriba
- Matthys Henndrik Botha
- Maylene Shung King
- Melanie Shaw
- Michael Held
- Michelle Williams
- Mimi Deetlefs
- Miriam Kahn
- Mogamat Yazied Chothia
- Moosa Parak
- Morna Cornell
- Muideen Bello
- Mumtaz Abbass
- Muzzammil Ismail
- Nabilah Ebrahim
- Naomi Levitt
- Natacha Berkowitz
- Nectarios Papavarnavas
- Nei-yuan Hsiao
- Nelesh Govender
- Nellis van Zyl Smit
- Nesbert Zinyakatira
- Neshaad Schrueder
- Nicki Tiffin
- Nicolene Du Toit
- Nienke van Schaik
- Nisha Jacob
- Nokubonga Vundla
- Nokwanda Sithole
- Nomonde Mbatani
- Nonte Mhlana
- Nosi Kalawe
- Ntobeko Ntusi
- Olina Ngwenya
- Pam Groenewald
- Patience Nyakato
- Patrick Szymanski
- Peter Beckwith
- Peter J Raubenheimer
- Petro Frankenfeld
- Phindile Gina
- Pierre Dane
- Pieter Venter
- Prasun Singh
- Priscilla Tsondai
- Raakhee Girran
- Rachel Carelse
- Remy Daroowala
- Rene Krogscheepers
- René English
- Renée de Waal
- Reshma Kassanjee
- Riaasat Khan
- Richard Osei-Yeboah
- Riezaah Abrahams
- Riyaadh Dawood
- Rizqa Armien
- Robert van den berg
- Rochelle Bailey
- Rochelle Grobler
- Rodgers Manganyi
- Rory Pienaar
- Ruan Marais
- Sa'ad Lahri
- Saadiq Kariem
- Saadiq Moolla
- Sabelo Hlatswayo
- Santhuri Viranna
- Scott Lee Jones
- Sean Burmeister
- Sean Wasserman
- Shakeel Hoosain
- Sharita Kooverjee
- Sheron Forgus
- Shiraaz Gabriel
- Simthandile Mazondwa
- Sipho Dlamini
- Sthembiso Sithole
- Sue Le Roux
- Sumanth Karamchand
- Susan Hugo
- Suzaan Marais
- Suzanne Kuhn
- Tarin Europa
- Taryn Allie
- Tendesayi Kufa
- Thabiet Jardine
- Thania Kahn
- Themba Mutemaringa
- Thina Majola
- Thoko Malaba
- Tholakele Sabela
- Thomas Crede
- Thomas Du Toit
- Tian Van Der Merwe
- Tsaone Tamuhla
- Usha Lalla
- Vanessa Lomas-Marais
- Vanessa Mudaly
- Vanessa Naidoo
- Virginia Zweigenthal
- Vuyolwethu Soldati
- Waasila Jassat
- Washiefa Isaacs
- Werner Viljoen
- Wilhelm Endres
- Yamanya Tembo
- Ziyanda Vundle
- Publication venue
- 'Oxford University Press (OUP)'
- Publication date
- 01/01/2021
- Field of study
Risk factors for coronavirus disease 2019 (COVID-19) death in sub-Saharan Africa and the effects of human immunodeficiency virus (HIV) and tuberculosis on COVID-19 outcomes are unknown. We conducted a population cohort study using linked data from adults attending public-sector health facilities in the
Western Cape, South Africa. We used Cox proportional hazards models, adjusted for age, sex, location, and comorbidities, to examine the associations between HIV, tuberculosis, and COVID-19 death from 1 March to 9 June 2020 among (1) public-sector “active patients” (≥1 visit in the 3 years before March 2020); (2) laboratory-diagnosed COVID-19 cases; and (3) hospitalized COVID-19
cases. We calculated the standardized mortality ratio (SMR) for COVID-19, comparing adults living with and without HIV using
modeled population estimates.Among 3 460 932 patients (16% living with HIV), 22 308 were diagnosed with COVID-19, of whom 625 died. COVID19 death was associated with male sex, increasing age, diabetes, hypertension, and chronic kidney disease. HIV was associated with
COVID-19 mortality (adjusted hazard ratio [aHR], 2.14; 95% confidence interval [CI], 1.70–2.70), with similar risks across strata of
viral loads and immunosuppression. Current and previous diagnoses of tuberculosis were associated with COVID-19 death (aHR,
2.70 [95% CI, 1.81–4.04] and 1.51 [95% CI, 1.18–1.93], respectively). The SMR for COVID-19 death associated with HIV was 2.39
(95% CI, 1.96–2.86); population attributable fraction 8.5% (95% CI, 6.1–11.1)
The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance
- Author
- Aanniz Tarek
- Abar Aden Elmi
- Abbad Anass
- Abdelfattah May
- Abdelhamid W.
- Abdella Saro
- Abdellah Faouzi
- Abdelmjid El Oualid
- Abdelmoula Fatma
- Abdelmoulah F.
- Abderrahmane Maaroufi
- Abderrazak Rfaki
- Abdillahi Idil Salah
- Abdissa Alemseged
- Abdo I.
- Abdoelraheem Mohamed Hassan
- Abe Haruka
- Abebe Markos
- Abechi Priscilla
- Abera Adugna
- Abi Rachid
- Abias Abe G.
- Abichu Getachew
- Abid N.
- Abid Nabil
- Abid Salma
- Abilio Nuro
- Abimbola Sharon
- Abo Shama Noura M
- Abosede O.
- Aboubaker Mohamed Houmed
- Abouelhoda Mohamed
- Abouelnaga S.
- Abualas Elamin
- Abubeker Ramalia Chabi
- Abuelenein Ehab Abdelkader
- Abuzaid Nadir
- Abworo Edward O.
- Ada Diosdado Odjama Nseng
- Addidle Michael
- Adegbola Richard
- Adegnika Akim A.
- Adegnika Ayola Akim
- Adel Abd Monaem
- Adeniji Johnson A.
- Adepetun Tomiwa
- Adesegun A.
- Adesuyi Omoare
- Adetifa Ifedayo M. O.
- Adewumi Olubusuyi M.
- Adewumi Oluwatimilehin
- Adewumi Praise
- Adeyemi Kayode T.
- Adhikari Bishwo N.
- Adil El Hamouchi
- Adjiratou Aissatou B. A.
- Adnew Bethlehem
- Adu Bright
- Adu Eric
- Adusei-Poku Mildred
- Agbenyo Seyram Bless
- Agbodzi B.
- Ageez A.M.
- Agoti Charles N.
- Aguenaou Hassan
- Agwa Sara Hassan
- Agweyu Ambrose
- Ahmed El Bara
- Ahmed Elhadi A.
- Ahmed Hatem
- Ahmed M.
- Ahmed O. S.
- Ahmed Rahma
- Ahmed Shymaa S.
- Ahmed Side Mohamed
- Ahmed Yahaya Ali
- Ahouidi Ambroise
- Ahuka-Mundeke Steve
- Ahumibe Anthony
- Ahyong Vida
- Aissam El Aliani
- Ajayi Nnennaya A.
- Ajibaye Solomon
- Ajili F.
- Ajogbasile F.V.
- Ajogbasile Fehintola V.
- Akanbi Olusola Anuoluwapo
- Akenji Blaise MBoringong
- Akhoud Youssef
- Akinola Monilade
- Akogbeto Petas
- Akoua-Koffi Chantal G.
- Akpede George O.
- Al Onifade Fattah
- Alain Etoundi Mballa
- Alaoui Afaf
- Alaoui-Amine Sanaa
- Alarif Ahlam
- Alaruusi A. M.
- Albert Mélanie
- AlEmam Adba Alfatih
- Alemayehu Dawit Hailu
- Alemu Fekadu
- Algheriani Raiva
- Ali Abraham
- Ali M.A.
- Ali Mohamed Ahmed
- Ali Mushal
- Ali Wael
- Alkarim Mona O.A.
- Allam Loubna
- Allam Mushal
- Allam Mushal
- Altamura Settimia
- Amar Narjis
- Amenga-Etego Lucas N.
- Amer K. E.
- Amer Khaled
- Amira Souissi
- Amoako Daniel G.
- Amoo Olufemi Samuel
- Amoth Patrick
- Amougou Marie
- Ampofo William K.
- Amuri Adrienne A.
- Amuzu Dominic S. Y.
- Amzazi Saaïd
- Anang Abraham Kwabena
- Anas Abbad
- Andeko Julia C.
- Andersen Kristian
- Andersson Hana Sofia
- Andika Brian
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- von Gottberg Anne
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- Wadi Amira Suliaman
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- Wahab Kolawole Wasiu
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- Wang Jing
- Wang Xiuhua
- Wani Gregory
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- Waruhiu Cecilia
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- Wawina-Bokalanga Tony
- Wayengera Misaki
- Wayoro Oby
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- Webby Richard
- Weldemariam A.G.
- Weldemariam Atsbeha G.
- Werno Anja
- Wiley Michael R.
- Wilkinson David
- Wilkinson Eduan
- Williamson Carolyn
- Wilson Shannon
- Woelfel R.
- Wohl Shirlee
- Wolh S.
- Wolter Nicole
- Wuduri Zara
- Wulf Ben
- Wurie I.
- Wurie Isatta
- Xavier Joicymara S.
- Xiang Zuzheng
- Xie Hong
- Yadouleton Anges
- Yadouleton Yahya
- Yakout Mostfa
- Yandoko Rivalyn Nakoune
- Yang Qi
- Yao Kouadio T.
- Yassine Kasmi
- Yassine Zouheir
- Yasuda Jiro
- Yatassaye Bouna
- Yeboah C.
- Yeboah Richmond
- Yeboah-Manu Dorothy
- Yemi Bright K.
- Yiaba Cyrus
- Yiaba Warren Cyrus
- Yu Popova A.
- Yusuf Nyam Itse
- Yusuf Yacob Mohamed
- Zablon J. Matoke D.
- Zablon J.O.
- Zaher Tarek Refaat Elnagdy Raafat
- Zahra El Ansari Fatima
- Zahran Manal Hamdy
- Zekri Abdel-Rahman N.
- Zhang J.
- Zhou Pei
- Zinyowera Sekesai
- Zong Minko O.
- Zongo Arsène
- Zorgani A.
- Zouaki Amal
- Zulu P.
- Zumla Ali
- Zumla Alimuddin
- Zuze Boitumelo
- Publication venue
- 'American Association for the Advancement of Science (AAAS)'
- Publication date
- 07/10/2022
- Field of study
INTRODUCTION
Investment in Africa over the past year with regard to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing has led to a massive increase in the number of sequences, which, to date, exceeds 100,000 sequences generated to track the pandemic on the continent. These sequences have profoundly affected how public health officials in Africa have navigated the COVID-19 pandemic.
RATIONALE
We demonstrate how the first 100,000 SARS-CoV-2 sequences from Africa have helped monitor the epidemic on the continent, how genomic surveillance expanded over the course of the pandemic, and how we adapted our sequencing methods to deal with an evolving virus. Finally, we also examine how viral lineages have spread across the continent in a phylogeographic framework to gain insights into the underlying temporal and spatial transmission dynamics for several variants of concern (VOCs).
RESULTS
Our results indicate that the number of countries in Africa that can sequence the virus within their own borders is growing and that this is coupled with a shorter turnaround time from the time of sampling to sequence submission. Ongoing evolution necessitated the continual updating of primer sets, and, as a result, eight primer sets were designed in tandem with viral evolution and used to ensure effective sequencing of the virus. The pandemic unfolded through multiple waves of infection that were each driven by distinct genetic lineages, with B.1-like ancestral strains associated with the first pandemic wave of infections in 2020. Successive waves on the continent were fueled by different VOCs, with Alpha and Beta cocirculating in distinct spatial patterns during the second wave and Delta and Omicron affecting the whole continent during the third and fourth waves, respectively. Phylogeographic reconstruction points toward distinct differences in viral importation and exportation patterns associated with the Alpha, Beta, Delta, and Omicron variants and subvariants, when considering both Africa versus the rest of the world and viral dissemination within the continent. Our epidemiological and phylogenetic inferences therefore underscore the heterogeneous nature of the pandemic on the continent and highlight key insights and challenges, for instance, recognizing the limitations of low testing proportions. We also highlight the early warning capacity that genomic surveillance in Africa has had for the rest of the world with the detection of new lineages and variants, the most recent being the characterization of various Omicron subvariants.
CONCLUSION
Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve. This is important not only to help combat SARS-CoV-2 on the continent but also because it can be used as a platform to help address the many emerging and reemerging infectious disease threats in Africa. In particular, capacity building for local sequencing within countries or within the continent should be prioritized because this is generally associated with shorter turnaround times, providing the most benefit to local public health authorities tasked with pandemic response and mitigation and allowing for the fastest reaction to localized outbreaks. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century
Discutindo a educação ambiental no cotidiano escolar: desenvolvimento de projetos na escola formação inicial e continuada de professores
- Author
- Abbaneo Duccio
- Abbiendi Giovanni
- Abbrescia Marcello
- Abdullin Salavat
- Acosta Darin
- Acosta Jhon Gabriel
- Adam Nadia
- Adam Wolfgang
- Adams Mark Raymond
- Adams Todd
- Adiguzel Aytul
- Adler Volker
- Adzic Petar
- Afanasiev Serguei
- Agostino Lorenzo
- Agram Jean-Laurent
- Aguilar-Benitez Manuel
- Aguilo Ernest
- Ahmad Muhammad
- Ahmed Ijaz
- Ahuja Sudha
- Akchurin Nural
- Akgun Bora
- Akgun Ugur
- Akin Ilina Vasileva
- Alagoz Enver
- Albajar Carmen
- Albayrak Elif Asli
- Albergo Sebastiano
- Albrow Michael
- Alcaraz Maestre Juan
- Aldaya Martin Maria
- Alexander James
- Aliev Takhmasib
- Almeida Nuno
- Alver Burak
- Alverson George
- Alves Gilvan
- Amapane Nicola
- Amsler Claude
- Anagnostou Georgios
- Anastassov Anton
- Anderson Jacob
- Anderson Michael
- Andrea Jeremy
- Andreev Valeri
- Andreev Vladimir
- Andreev Yuri
- Andrews Warren
- Anghel Ioana Maria
- Antonelli Louis
- Antunovic Zeljko
- Apanasevich Leonard
- Apollinari Giorgio
- Appelt Eric
- Apresyan Artur
- Aranyi Attila
- Arce Pedro
- Arcidiacono Roberta
- Arenton Michael Wayne
- Arfaei Hessamaddin
- Argiro Stefano
- Arisaka Katsushi
- Arneodo Michele
- Asaadi Jonathan
- Asghar Muhammad Irfan
- Askew Andrew
- Assran Yasser
- Ata Metin
- Atac Muzaffer
- Atramentov Oleksiy
- Attikis Alexandros
- Auffray Etiennette
- Autermann Christian Tobias
- Auzinger Georg
- Avery Paul
- Avetisyan Aram
- Azarkin Maksim
- Azhgirey Igor
- Aziz Tariq
- Azzi Patrizia
- Azzolini Virginia
- Azzurri Paolo
- Baarmand Marc M.
- Babb John
- Bacchetta Nicola
- Bachtis Michail
- Baden Drew
- Baesso Paolo
- Baffioni Stephanie
- Bagliesi Giuseppe
- Bai Yuting
- Baillon Paul
- Bainbridge Robert
- Bakhshiansohi Hamed
- Bakirci Mustafa Numan
- Bakken Jon Alan
- Balazs Michael
- Ball Austin
- Ball Gordon
- Ballin Jamie
- Ban Yong
- Banerjee Sudeshna
- Banerjee Sunanda
- Bansal Sunil
- Banzuzi Kukka
- Barbagli Giuseppe
- Barberis Emanuela
- Barbone Lucia
- Bardak Cemile
- Barfuss Anne-fleur
- Bargassa Pedrame
- Barge Derek
- Baringer Philip
- Barker Anthony
- Barnes Virgil E.
- Barnett Bruce Arnold
- Barney David
- Barone Luciano
- Barrett Matthew
- Bartalini Paolo
- Barth Christian
- Basegmez Suzan
- Basso Lorenzo
- Battilana Carlo
- Baty Clement
- Bauer Gerry
- Bauer Julia
- Bauerdick Lothar A. T.
- Baumgartel Darin
- Baur Ulrich
- Bazterra Victor Eduardo
- Bean Alice
- Beauceron Stephanie
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- Zablocki Jakub
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- Zalewski Piotr
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- Zang Jingjing
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- Zarubin Anatoli
- Zatserklyaniy Andriy
- Zeinali Maryam
- Zeise Manuel
- Zeuner Wolfram Dietrich
- Zeyrek Mehmet
- Zhang Jinzhong
- Zhang Linlin
- Zhang Zhen
- Zheng Yu
- Zhokin Alexander
- Zhu Bo
- Zhu Ren-Yuan
- Zhukov Valery
- Zhukova Victoria
- Ziebarth Eva Barbara
- Ziegler Jill
- Zielinski Marek
- Zito Giuseppe
- Zorbilmez Caglar
- Zotto Pierluigi
- Zou Wei
- Zumerle Gianni
- Zuranski Andrzej
- Zöller Marc Henning
- Publication venue
- Publication date
- 01/01/2011
- Field of study
A presente pesquisa buscou discutir como a Educação Ambiental (EA) vem sendo trabalhada, no Ensino Fundamental e como os docentes desta escola compreendem e vem inserindo a EA no cotidiano escolar., em uma escola estadual do município de Tangará da Serra/MT, Brasil. Para tanto, realizou-se entrevistas com os professores que fazem parte de um projeto interdisciplinar de EA na escola pesquisada. Verificou-se que o projeto da escola não vem conseguindo alcançar os objetivos propostos por: desconhecimento do mesmo, pelos professores; formação deficiente dos professores, não entendimento da EA como processo de ensino-aprendizagem, falta de recursos didáticos, planejamento inadequado das atividades. A partir dessa constatação, procurou-se debater a impossibilidade de tratar do tema fora do trabalho interdisciplinar, bem como, e principalmente, a importância de um estudo mais aprofundado de EA, vinculando teoria e prática, tanto na formação docente, como em projetos escolares, a fim de fugir do tradicional vínculo “EA e ecologia, lixo e horta”.Facultad de Humanidades y Ciencias de la Educació
stairs and fire
- Author
- Abbaneo Duccio
- Abbiendi Giovanni
- Abbrescia Marcello
- Abdullin Salavat
- Acosta Darin
- Acosta Jhon Gabriel
- Adair Antony
- Adam Nadia
- Adam Wolfgang
- Adams Mark Raymond
- Adams Todd
- Adiguzel Aytul
- Adler Volker
- Adzic Petar
- Afanasiev Serguei
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- Triantis Frixos A.
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- Verdier Patrice
- Verdini Piero Giorgio
- Veres Gabor Istvan
- Vergili Latife Nukhet
- Vergili Mehmet
- Verwilligen Piet
- Verzetti Mauro
- Veszpremi Viktor
- Vesztergombi Gyorgy
- Veverka Jan
- Vichoudis Paschalis
- Vidal Marono Miguel
- Vidal Richard
- Vila Ivan
- Vilar Cortabitarte Rocio
- Vilela Pereira Antonio
- Villasenor-Cendejas Luis Manuel
- Villella Ilaria
- Vinogradov Alexey
- Virdee Tejinder
- Viret Sébastien
- Vischia Pietro
- Vishnevskiy Dmitry
- Vitulo Paolo
- Viviani Claudio
- Vizan Garcia Jesus Manuel
- Vlasov Evgueni
- Vlimant Jean-Roch
- Vodopiyanov Igor
- Vogel Helmut
- Volkov Alexey
- Volobouev Igor
- Volodko Anton
- Volpe Roberta
- Vorobiev Igor
- Vorobyev Alexey
- Vorobyev Andrey
- Voutilainen Mikko
- Vuosalo Carl
- Vutova Mariana
- Wagner Philipp
- Wagner Stephen Robert
- Wagner-Kuhr Jeannine
- Wakefield Stuart
- Wallny Rainer
- Walsh Roberval
- Walsh Sinead
- Waltenberger Wolfgang
- Walzel Gerhard
- Wan Zongru
- Wang Dayong
- Wang Jian
- Wang Jian
- Wang Minzu
- Wang Siguang
- Wang Xianyou
- Wang Zheng
- Wardle Nicholas
- Wardrope David
- Wasserbaech Steven
- Wayne Mitchell
- Weber Hannsjoerg Artur
- Weber Hendrik
- Weber Martin
- Weber Matthias
- Wehrli Lukas
- Weiler Thomas
- Weinberg Marc
- Wendland Lauri
- Weng Joanna
- Weng Yao
- Wenger Edward Allen
- Werner Jeremy Scott
- West Christopher
- Wetzel James
- Whitbeck Andrew
- Whitmore Juliana
- Whyntie Tom
- Wickens John
- Wickramage Nadeesha
- Widl Edmund
- Wigmans Richard
- Wilken Rachel
- Wilkinson Richard
- Williams Grayson
- Williams Thomas
- Willmott Carlos
- Wimpenny Stephen
- Winn Dave
- Winstrom Lucas
- Wissing Christoph
- Wittich Peter
- Wittmer Bruno
- Wolf Matthias
- Wolf Roger
- Womersley William John
- Won Steven
- Wood Darien
- Wood Jeffrey Scott
- Wood John
- Worm Steven
- Wright Douglas
- Wrochna Grzegorz
- Wu Weimin
- Wulz Claudia-Elisabeth
- Wyslouch Bolek
- Wöhri Hermine Katharina
- Würthwein Frank
- Xiao Hong
- Xie Si
- Xu Ming
- Yagil Avraham
- Yalvac Metin
- Yang Fan
- Yang Mingming
- Yang Yong
- Yang Zong-Chang
- Yazgan Efe
- Yelton John
- Yetkin Taylan
- Yi Kai
- Yildirim Eda
- Yilmaz Yetkin
- Yohay Rachel
- Yoo Hwi Dong
- Yoo Jaehyeok
- Yoon Sungho
- York Andrew
- Yu Intae
- Yu Shin-Shan
- Yumiceva Francisco
- Yun Jae Chul
- Zabel James
- Zabi Alexandre
- Zablocki Jakub
- Zaganidis Nicolas
- Zakaria Mohammed
- Zalewski Piotr
- Zanetti Marco
- Zang Jingjing
- Zang Shi-Lei
- Zarubin Anatoli
- Zatserklyaniy Andriy
- Zeinali Maryam
- Zeise Manuel
- Zeuner Wolfram Dietrich
- Zeyrek Mehmet
- Zhang Jinzhong
- Zhang Zhen
- Zheng Yu
- Zhokin Alexander
- Zhu Bo
- Zhu Ren-Yuan
- Zhukov Valery
- Zhukova Victoria
- Ziebarth Eva Barbara
- Ziegler Jill
- Zielinski Marek
- Zito Giuseppe
- Zotto Pierluigi
- Zou Wei
- Zuranski Andrzej
- Zöller Marc Henning
- Publication venue
- Publication date
- 01/01/2012
- Field of study
Molecular detection of melanoma nodal metastases
- Author
- Publication venue
- Department of Medicine
- Publication date
- 01/01/2002
- Field of study
Includes bibliography.The aim of this study was to develop a practical and reproducible multi-marker RT-PCR essay, with the emphasis on achieving maximum specificity for the detection of melanoma nodal metastases. A novel protocol for the efficient homogenisation of nodal tissue was developed, with clinical applicability as the objective
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