Producción y calidad de forraje de siete variedades . Hibridas de sorgo

La ganadería que se desarrolla en la región sur de Jalisco, presenta marcadas fluctuaciones a través del año por la estacionalidad en la producción forrajera y el alto índice de explotaciones extensivas

MEXICAN WILD LUPINES AS A SOURCE OF QUINOLIZIDINE ALKALOIDS OF ECONOMIC POTENTIAL

Quinolizidine alkaloids such as lupanine,13-hydroxylupanine, multiflorine, angustifoline and sparteine, which are present inthe species of the genus Lupinus, have beenreported to have biopesticide and pharmacological activities. The aim of this studywas to quantify the content and variationof the individual alkaloids in seeds of L.mexicanus, L. exaltatus, L. montanus andL. stipulatus collected in different states ofMexico. Lupanine was the major (5.05 ±0.37 mg/g) alkaloid found in L. mexicanus,whereas sparteine was the main alkaloidpresent in L. montanus (3.97 ± 0.49 mg/g).Conversely, L. stipulatus contained onlysmall quantities of lupanine and sparteine(0.1 ± 0.002 and 0.04 ± 0.01 mg/g, respectively). Angustifoline was detected only in L.montanus, but in a very low amount (0.048± 0.03). The results of this study indicatethat L. mexicanus and L. montanus can beconsidered as important sources of lupanine and sparteine for their use as natural pesticide or pharmacological agents.Los alcaloides quinolizidinicos lupanina,13-hidroxilupanina, multiflorina, angustifolina y esparteina, presentes en el géneroLupinus poseen actividades bioplagiciday farmacológica. El objetivo del presenteestudio fue cuantificar el contenido y variación de los alcaloides mencionados ensemillas de L. mexicanus, L. exaltatus, L.montanus y L. stipulatus, colectados endiferentes estados de México. La lupaninafue el principal (5.05 ± 0.37mg /g) alcaloideencontrado en L. mexicanus, mientras que laesparteína fue el mayor alcaloide presente en L. montanus (3.97 ± 0.49 mg/g). Sinembargo, en L. stipulatus se encontraron pequeñas cantidades de lupanina y esparteína(0.1 ± 0.002 y 0.04 ± 0.01 mg/g, respectivamente). La angustifolina sólo se detectóen bajos niveles en L. montanus (0.048 ±0.03). Los resultados de este estudio indicanque L. mexicanus y L. montanus pueden serconsiderados como una fuente importantede lupanina y esparteína, los cuales puedenser utilizados como pesticidas o hipoglucé-micos naturales

Catatonia: Clinical overview of the diagnosis, treatment, and clinical challenges

Catatonia is a syndrome that has been associated with several mental illness disorders but that has also presented as a result of other medical conditions. Schizophrenia and other psychiatric disorders such as mania and depression are known to be associated with catatonia; however, several case reports have been published of certain medical conditions inducing catatonia, including hypo-natremia, cerebral venous sinus thrombosis, and liver transplantation. Neuroleptic Malignant Syndrome and anti-NMDA receptor encephalitis are also prominent causes of catatonia. Patients taking benzodiazepines or clozapine are also at risk of developing catatonia following the withdrawal of these medications—it is speculated that the prolonged use of these medications increases gamma-aminobutyric acid (GABA) activity and that discontinuation may increase excitatory neurotrans-mission, leading to catatonia. The treatment of catatonia often involves the use of benzodiazepines, such as lorazepam, that can be used in combination therapy with antipsychotics. Definitive treatment may be found with electroconvulsive therapy (ECT). Aberrant neuronal activity in different motor pathways, defective neurotransmitter regulation, and impaired oligodendrocyte function have all been proposed as the pathophysiology behind catatonia. There are many clinical challenges that come with catatonia and, as early treatment is associated with better outcomes, it becomes imperative to understand these challenges. The purpose of this manuscript is to provide an overview of these challenges and to look at clinical studies regarding the pathophysiology, diagnosis, and treatment of as well as the complications and risk factors associated with catatonia

Assisted evolution enables HIV-1 to overcome a high trim5α-imposed genetic barrier to rhesus macaque tropism

Diversification of antiretroviral factors during host evolution has erected formidable barriers to cross-species retrovirus transmission. This phenomenon likely protects humans from infection by many modern retroviruses, but it has also impaired the development of primate models of HIV-1 infection. Indeed, rhesus macaques are resistant to HIV-1, in part due to restriction imposed by the TRIM5α protein (rhTRIM5α). Initially, we attempted to derive rhTRIM5α-resistant HIV-1 strains using two strategies. First, HIV-1 was passaged in engineered human cells expressing rhTRIM5α. Second, a library of randomly mutagenized capsid protein (CA) sequences was screened for mutations that reduced rhTRIM5α sensitivity. Both approaches identified several individual mutations in CA that reduced rhTRIM5α sensitivity. However, neither approach yielded mutants that were fully resistant, perhaps because the locations of the mutations suggested that TRIM5α recognizes multiple determinants on the capsid surface. Moreover, even though additive effects of various CA mutations on HIV-1 resistance to rhTRIM5α were observed, combinations that gave full resistance were highly detrimental to fitness. Therefore, we employed an 'assisted evolution' approach in which individual CA mutations that reduced rhTRIM5α sensitivity without fitness penalties were randomly assorted in a library of viral clones containing synthetic CA sequences. Subsequent passage of the viral library in rhTRIM5α-expressing cells resulted in the selection of individual viral species that were fully fit and resistant to rhTRIM5α. These viruses encoded combinations of five mutations in CA that conferred complete or near complete resistance to the disruptive effects of rhTRIM5α on incoming viral cores, by abolishing recognition of the viral capsid. Importantly, HIV-1 variants encoding these CA substitutions and SIVmac239 Vif replicated efficiently in primary rhesus macaque lymphocytes. These findings demonstrate that rhTRIM5α is difficult to but not impossible to evade, and doing so should facilitate the development of primate models of HIV-1 infection

Proyecto de exportación de los insumos, equipos y materiales relacionados con la masa de maíz hacia la Unión Europea

El proyecto de internacionalización de Lusio se basó en un alcance de la investigación en la Unión Europea con los objetivos específicos que tenían un enfoque en los siguientes siete apartados: empresa, producto, mercado, comercialización, logística, legal y finanzas. Dentro de la administración del proyecto, se buscó una planeación, organización y dirección de los recursos disponibles para el cumplimiento de las metas y objetivos del plan desarrollado. En su proceso, se contó con distintos referentes teóricos que ayudaron a tomar decisiones fundamentadas para la comprensión de las necesidades de la empresa y su intervención. Parte de su metodología se basó en una división de los entregables del trabajo dentro del equipo para su elaboración tanto de manera presencial como trabajo en línea haciendo uso de plataformas para la constante comunicación. Así mismo, en los resultados encontrados, se resaltan las características principales de la línea de productos a distribuir en el mercado internacional, investigación de mercado enfocada en Alemania y España, estrategias de distribución emparejadas a las de la competencia para crear ventajas competitivas, un plan logístico, la presentación de documentos legales como contratos de compraventa y de transporte y, al finalizar, se presentaron conclusiones y recomendaciones que se fueron encontrando con el seguimiento del proyecto para la creación de estrategias que posicionen a Lusio en el mercado europeo, aprovechando la oportunidad que existe en estos mercados.ITESO, A.C

Btla signaling in conventional and regulatory lymphocytes coordinately tempers humoral immunity in the intestinal mucosa

The Btla inhibitory receptor limits innate and adaptive immune responses, both preventing the development of autoimmune disease and restraining anti-viral and anti-tumor responses. It remains unclear how the functions of Btla in diverse lymphocytes contribute to immunoregulation. Here, we show that Btla inhibits activation of genes regulating metabolism and cytokine signaling, including Il6 and Hif1a, indicating a regulatory role in humoral immunity. Within mucosal Peyer\u27s patches, we find T-cell-expressed Btla-regulated Tfh cells, while Btla in T or B cells regulates GC B cell numbers. Treg-expressed Btla is required for cell-intrinsic Treg homeostasis that subsequently controls GC B cells. Loss of Btla in lymphocytes results in increased IgA bound to intestinal bacteria, correlating with altered microbial homeostasis and elevations in commensal and pathogenic bacteria. Together our studies provide important insights into how Btla functions as a checkpoint in diverse conventional and regulatory lymphocyte subsets to influence systemic immune responses

NIRCam coronagraphic Lyot stop: design, fabrication, and testing

The NIRCam instrument on the James Webb Space Telescope (JWST) will provide a coronagraphic imaging capability to search for extrasolar planets in the 2 - 5 microns wavelength range. This capability is realized by a set of Lyot pupil stops with patterns matching the occulting mask located in the JWST intermediate focal plane in the NIRCam optical system. The complex patterns with transparent apertures are made by photolithographic process using a metal coating in the opaque region. The optical density needs to be high for the opaque region, and transmission needs to be high at the aperture. In addition, the Lyot stop needs to operate under cryogenic conditions. We will report on the Lyot stop design, fabrication and testing in this paper

Conformational adaptation of Asian macaque TRIMCyp directs lineage specific antiviral activity

TRIMCyps are anti-retroviral proteins that have arisen independently in New World and Old World primates. All TRIMCyps comprise a CypA domain fused to the tripartite domains of TRIM5α but they have distinct lentiviral specificities, conferring HIV-1 restriction in New World owl monkeys and HIV-2 restriction in Old World rhesus macaques. Here we provide evidence that Asian macaque TRIMCyps have acquired changes that switch restriction specificity between different lentiviral lineages, resulting in species-specific alleles that target different viruses. Structural, thermodynamic and viral restriction analysis suggests that a single mutation in the Cyp domain, R69H, occurred early in macaque TRIMCyp evolution, expanding restriction specificity to the lentiviral lineages found in African green monkeys, sooty mangabeys and chimpanzees. Subsequent mutations have enhanced restriction to particular viruses but at the cost of broad specificity. We reveal how specificity is altered by a scaffold mutation, E143K, that modifies surface electrostatics and propagates conformational changes into the active site. Our results suggest that lentiviruses may have been important pathogens in Asian macaques despite the fact that there are no reported lentiviral infections in current macaque populations

Community Water Model CWatM Manual

With a growing population and economic development, it is expected that water demands will increase significantly in the future, especially in developing regions. At the same time, climate change is expected to alter spatial patterns of precipitation and temperature and will have regional to localized impacts on water availability. Thus, it is important to assess water demand, water supply and environmental needs over time to identify the populations and locations that will be most affected by these changes linked to water scarcity, droughts and floods. The Community Water Model will be designed for this purpose in that they include an accounting of how future water demands will evolve in response to socioeconomic change and how water availability will change in response to climate. CWatM will represent one of the new key elements of the WAT program going forward and increasing the innovative niche of work. We will use and develop the model to work at both global and regional (basin) level. The configuration of the model is open source and community-driven to promote our work amongst the wider water community and is flexible enough to introduce further planned developments such as water quality and hydro-economy. Our vision for short to medium term work of the group is to introduce water quality (i.e., salinization in deltas and eutrophication associated with mega cities) into the community model and to consider how to include a qualitative/quantitative measure of transboundary river and groundwater governance into a scenario and modelling framework