101 research outputs found
Geodesic defect anchoring on nematic shells
Nematic shells are colloidal particles coated with nematic liquid crystal molecules, which may freely glide and rotate on the colloid's surface while keeping their long axis on the local tangent plane. Molecular dynamics simulations on a nanoscopic spherical shell indicate that under appropriate adhesion conditions for the molecules on the equator, the equilibrium nematic texture exhibits at each pole a pair of +1/2 defects so close to one another to be treated as one +1 defect. Spirals connect the polar defects, though the continuum limit of the interaction potential would not feature any elastic anisotropy. A molecular averaging justifies an anchoring defect energy that feels the geodesics emanating from the defect. All our observations are explained by such a geodesic anchoring, which vanishes on flat manifolds
Lifting ordered surfaces : ellipsoidal nematic shells
When a material surface is functionalized so as to acquire some type of order, functionalization of which soft condensed matter systems have recently provided many interesting examples, the modeller faces an alternative. Either the order is described on the curved, physical surface where it belongs, or it is described on a flat surface that is unrolled as pre-image of the physical surface under a suitable height function. This paper applies a general method that pursues the latter avenue by lifting whatever order tensor is deemed appropriate from a flat to a curved surface. We specialize this method to nematic shells, for which it also provides a simple, but perhaps convincing interpretation of the outcomes of some molecular-dynamics experiments on ellipsoidal shells
The burden of Candida species colonization in NICU patients: a colonization surveillance study
Fungal infections are an important cause
of morbidity and mortality in neonatal
intensive care units (NICUs). The identification
of specific risk factors supports prevention
of candidemia in neonates. Effective
prophylactic strategies have recently
become available, but the identification
and adequate management of high-risk infants
is still a priority. Prior colonization is
a key risk factor for candidemia. For this
reason, surveillance studies to monitor incidence,
species distribution, and antifungal
susceptibility profiles, are mandatory.
Among 520 infants admitted to our NICU
between January 2013 and December
2014, 472 (90.77%) were included in the
study. Forty-eight out of 472 (10.17%) patients
tested positive for Candida spp. (C.),
at least on one occasion. All the colonized
patients tested positive for the rectal swab,
whereas 7 patients also tested positive for
the nasal swab. Fifteen out of 472 patients
(3.18%) had more than one positive rectal
or nasal swab during their NICU stay.
Moreover, 9 out of 15 patients tested negative
at the first sampling, suggesting they
acquired Candida spp. during their stay.
Twenty-five of forty-eight (52.1%) colonized
patients carried C.albicans and 15/48
(31.25%) C.parapsilosis. We identified as
risk factors for Candida spp. colonization:
antibiotic therapy, parenteral nutrition,
the use of a central venous catheter, and
nasogastric tube. Our experience suggests
that effective microbiological surveillance
can allow for implementing proper, effective
and timely control measures in a highrisk
setting
CARATTERI IDROGEOLOGICI DELLE SORGENTI CIRCOSTANTI IL LAGO DI LESINA (GARGANO)
Si studia il contributo alla Laguna di Lesina proveniente da tre principali sorgenti alimentate dal vasto acquifero carsico del Gargano. Con un approccio idrologico-statistico si determina l'estensione dell'area di alimentazione delle sorgenti
Parity Breaking in Nematic Tactoids
We theoretically investigate under what conditions the director field in a
spindle-shaped nematic droplet or tactoid obtains a twisted, parity-broken
structure. By minimizing the sum of the bulk elastic and surface energies, we
show that a twisted director field is stable if the twist and bend elastic
constants are small enough compared to the splay elastic constant, but only if
the droplet volume is larger than some minimum value. We furthermore show that
the transition from an untwisted to a twisted director-field structure is a
sharp function of the various control parameters. We predict that suspensions
of rigid, rod-like particles cannot support droplets with a parity broken
structure, whereas they could possibly occur in those of semi-flexible,
worm-like particles.Comment: 20 pages, 9 figures, submitted to Journal of Physics: Condensed
Matte
Drug-eluting balloons for the treatment of the superficial femoral artery in-stent restenosis: 2-year follow-up.
OBJECTIVES:
The aim of this prospective registry was to evaluate the safety and efficacy at 2-year follow-up of the use of drug-eluting balloons (DEBs) for the treatment of superficial femoral artery (SFA) in-stent restenosis (ISR).
BACKGROUND:
The use of DEBs for the treatment of SFA ISR is associated with a satisfactory primary patency rate at 1 year, but no data are available for longer follow-up. Unfortunately, when DEBs were used to treat SFA de novo lesions, the occurrence of restenosis increased by 50% between the first and the second years of follow-up.
METHODS:
From December 2009 to December 2010, 39 consecutive patients underwent percutaneous transluminal angioplasty of SFA ISR at our institution (Clinica Montevergine, Mercogliano, Italy). All patients underwent conventional SFA percutaneous transluminal angioplasty and final post-dilation with paclitaxel-eluting balloons (IN.PACT, Medtronic Inc., Minneapolis, Minnesota). Patients were evaluated for up to 24 months.
RESULTS:
During follow-up, 1 patient died of heart failure and another of sudden death, for a 2-years rate of cardiovascular mortality rate of 5.12 %. The primary patency rate at 2 years was 70.3% (11 of 37 patients experienced restenosis recurrence at 2-year follow-up). The treatment of complex ISR lesions (classes II and III) was associated with an increased rate of recurrent restenosis compared with class I (33.3 % and 36.3 % vs. 12.5%; p = 0.05).
CONCLUSIONS:
The data suggest that adjunctive use of DEBs for the treatment of SFA ISR is a safe and effective therapeutic strategy up to 2 years of follow-up
AMPK-dependent phosphorylation of MTFR1L regulates mitochondrial morphology
Mitochondria are dynamic organelles that undergo membrane remodeling events in response to metabolic alterations to generate an adequate mitochondrial network. Here, we investigated the function of mitochondrial fission regulator 1-like protein (MTFR1L), an uncharacterized protein that has been identified in phosphoproteomic screens as a potential AMP-activated protein kinase (AMPK) substrate. We showed that MTFR1L is an outer mitochondrial membrane-localized protein modulating mitochondrial morphology. Loss of MTFR1L led to mitochondrial elongation associated with increased mitochondrial fusion events and levels of the mitochondrial fusion protein, optic atrophy 1. Mechanistically, we show that MTFR1L is phosphorylated by AMPK, which thereby controls the function of MTFR1L in regulating mitochondrial morphology both in mammalian cell lines and in murine cortical neurons in vivo. Furthermore, we demonstrate that MTFR1L is required for stress-induced AMPK-dependent mitochondrial fragmentation. Together, these findings identify MTFR1L as a critical mitochondrial protein transducing AMPK-dependent metabolic changes through regulation of mitochondrial dynamics.</p
Stroma-derived miR-214 coordinates tumor dissemination
BackgroundTumor progression is based on a close interaction between cancer cells and Tumor MicroEnvironment (TME). Here, we focus on the role that Cancer Associated Fibroblasts (CAFs), Mesenchymal Stem Cells (MSCs) and microRNAs (miRs) play in breast cancer and melanoma malignancy.MethodsWe used public databases to investigate miR-214 expression in the stroma compartment of primary human samples and evaluated tumor formation and dissemination following tumor cell injections in miR-214 overexpressing (miR-214(over)) and knock out (miR-214(ko)) mice. In addition, we dissected the impact of Conditioned Medium (CM) or Extracellular Vesicles (EVs) derived from miR-214-rich or depleted stroma cells on cell metastatic traits.ResultsWe evidence that the expression of miR-214 in human cancer or metastasis samples mostly correlates with stroma components and, in particular, with CAFs and MSCs. We present data revealing that the injection of tumor cells in miR-214(over) mice leads to increased extravasation and metastasis formation. In line, treatment of cancer cells with CM or EVs derived from miR-214-enriched stroma cells potentiate cancer cell migration/invasion in vitro. Conversely, dissemination from tumors grown in miR-214(ko) mice is impaired and metastatic traits significantly decreased when CM or EVs from miR-214-depleted stroma cells are used to treat cells in culture. Instead, extravasation and metastasis formation are fully re-established when miR-214(ko) mice are pretreated with miR-214-rich EVs of stroma origin. Mechanistically, we also show that tumor cells are able to induce miR-214 production in stroma cells, following the activation of IL-6/STAT3 signaling, which is then released via EVs subsequently up-taken by cancer cells. Here, a miR-214-dependent pro-metastatic program becomes activated.ConclusionsOur findings highlight the relevance of stroma-derived miR-214 and its release in EVs for tumor dissemination, which paves the way for miR-214-based therapeutic interventions targeting not only tumor cells but also the TME
An outbreak of Portuguese man-of-war (Physalia physalis - Linnaeus, 1758) envenoming in Southeastern Brazil
Formation Regularities of Plasmonic Silver Nanostructures on Porous Silicon for Effective Surface-Enhanced Raman Scattering
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