Method development and validation of meropenem in pharmaceutical dosage form by RP-HPLC

441-446An analytical reversed phase HPLC (RP-HPLC) method has been developed and validated for the estimation of meropenem using RP-HPLC column [Inertsil C18 (4.6 mm × 250 mm) 5µ]. The validation parameters has been followed as per FDA and ICH specifications under the study. Water, acetonitrile and methanol have been used as mobile phases in specific composition. The pH of the mobile phase is adjusted with phosphoric acid. The isocratic flow rate of the mobile phase is 1.5 mL/min with UV detection of 300 nm. The method is validated for accuracy, linearity and precision with analytical samples. Moreover, the specificity of the method has determined by the comparison of the drug content in a marketed preparation of meropenem solution. The linearity has been found to be more than 0.999 as well as the precision and accuracy values are less than 2% and from 95% to 105%, respectively. The developed and validated method is found to be the most suitable for quality control programs and can be used as fast, economic, accurate and reproducible

Structural characterization of Himalayan black rock salt by SEM, XRD and in-vitro antioxidant activity

International audienceSalt is an essential component of daily life that balances the physiological functions of the human body as well as other living systems. Different varieties of salts are available in the global market. Out of many salt varieties, Himalayan rock salts have gained tremendous importance among consumers due to its diverse nutritional and medicinal properties. There are two types of Himalayan rock salts. One is the Himalayan pink salt and the other one is Himalayan black salt. Out of the two, the Himalayan pink salt is studied extensively but the black salt is underexplored. In the present study, the Himalayan black rock salt was explored to generate more scientific evidence in terms of its geochemical characterization using FE-SEM, X-ray diffractometry (XRD), elemental content using atomic absorption spectrophotometry (AAS) and in vitro antioxidant activity by different methods for the first time. The study revealed that Himalayan black salt was irregular in shape with a quadrilateral, cubic, irregular crystalline structure. The Himalayan black salt exhibited antioxidant effect and interestingly showed low Na levels than common sea salt and Himalayan pink salt. The Himalayan black salt also contained important minerals like iron, calcium and magnesium which are beneficial to human health thus exhibiting superior characteristics over other conventional table salts

Pharmaceutical compounds in drinking water

Pharmaceutical products and their wastes play a major role in the degradation of environment. These drugs have positive as well as negative consequences on different environmental components including biota in different ways. Many types of pharmaceutical substances have been detected with significant concentrations through various advanced instrumental techniques in surface water, subsurface water, ground water, domestic waste water, municipal waste water and industrial effluents. The central as well as state governments in India are providing supports by creating excise duty free zones to promote the pharmaceutical manufacturers for their production. As a result, pharmaceutical companies are producing different types of pharmaceutical products at large scale and also producing complex non-biodegradable toxic wastes byproducts and releasing untreated or partially treated wastes in the environment in absence of strong regulations. These waste pollutants are contaminating all types of drinking water sources. The present paper focuses on water quality pollution by pharmaceutical pollutants, their occurrences, nature, metabolites and their fate in the environment