A new focus of spotted fever caused by Rickettsia parkeri in Brazil

Spotted fever (SF) is a tick-borne zoonosis caused by bacteria of the genus Rickettsia . The disease varies in severity from mild clinical forms to severe cases. In Brazil, Rickettsia rickettsii SF is the most serious rickettsiosis and can result in death if not diagnosed and treated at the onset of symptoms. The SF mild form is caused by Rickettsia parkeri strain Atlantic Rainforest, and this etiological agent has been reported in the South, Southeast and Northeast regions of the country, in areas of preserved or little antropized Atlantic Rainforest. Amblyomma ovale is the proven vector and dogs are the hosts associated with the bioagent cycle. During a SF case investigation in Paraty municipality, Rio de Janeiro State, an Atlantic Rainforest biome area in Southeastern Brazil, the human pathogen R. parkeri strain Atlantic Rainforest was detected by PCR in a sample of human skin inoculation eschar and in a female A. ovale tick collected from a dog. These results expand the known area of occurrence of this mild form rickettsiosis in Brazil. In addition, the results of the present study indicate the importance of implementing programs to control canine ectoparasites and to raise awareness of the risks of infection, signs and symptoms of SF caused by R. parkeri strain Atlantic Rainforest

Extrato bruto de bactéria Teredinibacter turnera, processo de obtenção do extrato bruto, composição farmacêutica contendo o mesmo e uso no tratamento de distúrbios e desordens proliferativas

ConcedidaA presente invenção se refere ao extrato bruto de bactéria Teredinibacter turnera atuante como agente antiproliferativo e antiviral, empregado em uma composição farmacêutica para o uso em tratamento de distúrbios e desordens proliferativas em mamíferos e infecções celulares e virais

Bacteria associated with Amblyomma cajennense tick eggs

Submitted by sandra infurna ([email protected]) on 2016-01-27T14:33:00Z No. of bitstreams: 1 vinicius_vizzoni_etal_IOC_2015.pdf: 961884 bytes, checksum: 288cfe6256eb763f41b7c1434a7e34d4 (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-01-27T14:46:50Z (GMT) No. of bitstreams: 1 vinicius_vizzoni_etal_IOC_2015.pdf: 961884 bytes, checksum: 288cfe6256eb763f41b7c1434a7e34d4 (MD5)Made available in DSpace on 2016-01-27T14:46:50Z (GMT). No. of bitstreams: 1 vinicius_vizzoni_etal_IOC_2015.pdf: 961884 bytes, checksum: 288cfe6256eb763f41b7c1434a7e34d4 (MD5) Previous issue date: 2015Universidade Federal do Rio de Janeiro. Instituto de Biologia. Departamento de Genética. Laboratório de Genética Molecular de Eucariontes e Simbiontes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biologia. Departamento de Genética. Laboratório de Genética Molecular de Eucariontes e Simbiontes. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência Nacional em Vetores das Riquetsioses. Rio de Janeiro, RJ, Brasil.Centers for Disease Control and Prevention. Division of Vector-Borne Diseases. Bacterial Diseases Branch. Fort Collins, CO, USA..Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência Nacional em Vetores das Riquetsioses. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biologia. Departamento de Genética. Laboratório de Genética Molecular de Eucariontes e Simbiontes. Rio de Janeiro, RJ, Brasil.Ticks represent a large group of pathogen vectors that blood feed on a diversity of hosts. In the Americas, the Ixodidae ticks Amblyomma cajennense are responsible for severe impact on livestock and public health. In the present work, we present the isolation and molecular identification of a group of culturable bacteria associated with A. cajennense eggs from females sampled in distinct geographical sites in southeastern Brazil. Additional comparative analysis of the culturable bacteria from Anocentor nitens, Rhipicephalus sanguineus and Ixodes scapularis tick eggs were also performed. 16S rRNA gene sequence analyses identified 17 different bacterial types identified as Serratia marcescens, Stenotrophomonas maltophilia, Pseudomonas fluorescens, Enterobacter spp., Micrococcus luteus, Ochrobactrum anthropi, Bacillus cereus and Staphylococcus spp., distributed in 12 phylogroups. Staphylococcus spp., especially S. sciuri, was the most prevalent bacteria associated with A. cajennense eggs, occurring in 65% of the samples and also frequently observed infecting A. nitens eggs. S. maltophilia, S. marcescens and B. cereus occurred infecting eggs derived from specific sampling sites, but in all cases rising almost as pure cultures from infected A. cajennense eggs. The potential role of these bacterial associations is discussed and they possibly represent new targets for biological control strategies of ticks and tick borne diseases

Rickettsia amblyommii infecting Amblyomma sculptum in endemic spotted fever area from southeastern Brazil

The Rickettsia bacteria include the aetiological agents for the human spotted fever (SF) disease. In the present study, a SF groupRickettsia amblyommii related bacterium was detected in a field collected Amblyomma sculptum (Amblyomma cajennense species complex) tick from a Brazilian SF endemic site in southeastern Brazil, in the municipality of Juiz de Fora, state of Minas Gerais. Genetic analysis based on genes ompA,ompB and htrA showed that the detected strain, named R. amblyommii str. JF, is related to the speciesR. amblyommii

Comparative growth of spotted fever group Rickettsia spp. strains in Vero cells

Submitted by Sandra Infurna ([email protected]) on 2016-08-17T14:09:39Z No. of bitstreams: 1 diego_lopez_etal_IOC_2016.pdf: 2701337 bytes, checksum: 305d815c1ea7388764ecabb822e50305 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2016-08-17T14:26:44Z (GMT) No. of bitstreams: 1 diego_lopez_etal_IOC_2016.pdf: 2701337 bytes, checksum: 305d815c1ea7388764ecabb822e50305 (MD5)Made available in DSpace on 2016-08-17T14:26:44Z (GMT). No. of bitstreams: 1 diego_lopez_etal_IOC_2016.pdf: 2701337 bytes, checksum: 305d815c1ea7388764ecabb822e50305 (MD5) Previous issue date: 2016Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de Pós-Graduação em Biodiversidade e Saúde. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência Nacional em Vetores das Riquetsioses. Rio de Janeiro, RJ, Brasil.Fundação Ezequiel Dias. Serviço de Virologia e Riquetsioses. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência Nacional em Vetores das Riquetsioses. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biologia. Laboratório de Genética Molecular de Eucariontes e Simbiontes. Rio de Janeiro, RJ, Brasil.Fundação Ezequiel Dias. Serviço de Virologia e Riquetsioses. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência Nacional em Vetores das Riquetsioses. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil .Universidade Estadual do Maranhão. Curso de Medicina Veterinária. Laboratório de Parasitologia. São Luís, MA, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biologia. Laboratório de Genética Molecular de Eucariontes e Simbiontes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biologia. Laboratório de Genética Molecular de Eucariontes e Simbiontes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de Pós-Graduação em Biodiversidade e Saúde. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência Nacional em Vetores das Riquetsioses. Rio de Janeiro, RJ, Brasil.In Brazil, the spotted fever group (SFG) Rickettsia rickettsii and Rickettsia parkeri related species are the etiological agents of spotted fever rickettsiosis. However, the SFG, Rickettsia rhipicephali, that infects humans, has never been reported. The study of growth dynamics can be useful for understanding the infective and invasive capacity of these pathogens. Here, the growth rates of the Brazilian isolates R. rickettsii str. Taiaçu, R. parkeri str. At#24, and R. rhipicephali HJ#5, were evaluated in Vero cells by quantitative polymerase chain reaction. R. rhipicephali showed different kinetic growth compared to R. rickettsii and R. parkeri