Agrarian distress and livelihood strategies : a study in Pulpalli Panchayat, Wayanad District, Kerala

This paper examines the household livelihood strategies under agrarian distress in Pulpalli Panchayat of Kerala. It also looks at the relationship between household assets and livelihood strategies. The negotiations of institutions by the marginalized and depressed sections of the society were analysed in detail. Major causes of agrarian distress in the study area are the ecological degradation and fall in crops income. Land continues to be the most important asset determining livelihood outcomes. The livelihood strategies have been investigated in relation to land, education, housing pattern, investments & credit facilities, and participation in organizational activities. The livelihood strategies adopted by farmers in the wake of agrarian crisis includes diversification of agriculture, share cropping, organic farming, self-help group activities, cattle rearing, migration and exchange of labour. Livelihood strategies varied across socio-economic groups as farmers owning better landholdings diversified cropping patterns while poor households participated in the activities of SHGs. Casual agricultural labourers and marginal farmers moved to other places in search of jobs. Mitigation of agrarian distress requires public provision of education, health and other social safety measures. Key words: Livelihood risk, Coping Strategies, Livelihood, Livelihood Assets, Institutions, Wayanad, Kerala JEL Classification: Q, Q 0

Synthesis and Characterization of Transition Metal Complexes of 2-Benzoylpyridine benzoylhydrazone

Cobalt(II), zinc(II) and mercury(II) complexes have been synthesized by the reaction of cobalt chloride, zinc chloride, zinc bromide and mercuric chloride with 2- benzoylpyridine benzoylhydrazone and characterized by analytical and spectral studies

Socio-economic analysis of finfish culture in cages in coastal waters of Kerala, southwest coast of India

Cage culture of finfish is becoming a popular alternate source of livelihood in Vembanad Lake in Kerala, India. A study was conducted during November to December, 2016 to assess the socio-economic status of the farmers practicing cage culture in Kerala and the resultant economic benefits. Data on demographic features, farming activities, specific culture and farm management practices, economics and problems related to cage farming were collected from 36 active farmers of the Ernakulam District. Etroplus suratensis (Pearl spot), Lates calcarifer (Asian sea bass), and Genetically Improved Farm Tilapia (GIFT) were the major species culture

Sterol Composition of the Indian Green Lipped Mussel Perna virdis

Sterol composition of green lipped mussel Perna virdis was analysed using GCMS. Cholesterol was found to be the dominant sterol (54.162% of the total sterol content). Other sterols such as Cholesta-5,22-dien-3-ol (3β), ergosta-5,22-dien-3-ol (3 β,22E, 24S),  26,26-dimethyl-5,24 (28)-Ergostadien-3β-ol, 26-nor-5cholesten- 3β-ol, stigmasterol and γ sitosterol were also detected. Presence of phytosterols like stigmasterol and γ sitosterol underlines high nutritional potential and food value of this bivalve mollusk

Virological, immunological and pathological findings of transplacentally transmitted bluetongue virus serotype 1 in IFNAR1-blocked mice during early and mid gestation

© 2020, The Author(s). Transplacental transmission (TPT) of wild-type Indian BTV-1 had never been experimentally proved. This study was first time investigated TPT of Indian BTV-1 (isolated from aborted and stillborn goat fetal spleens). The sequential pathology, virological and immune cell kinetics (CD4+, CD8+ T-lymphocytes and NK cells in spleen and PBMCs), and apoptosis in IFNAR1-blocked pregnant mice during early (infected on 1 GD) and mid (infected on 8 GD) gestation have been studied. There was higher rate of TPT during mid stage (71.43%) than early (57.14%) stage. In early stage reduced implantation sites, early embryonic deaths, abortions, and necro-haemorrhagic lesions had observed. Mid stage, congenital defects and neurological lesions in foetuses like haemorrhages, diffuse cerebral edema, necrotizing encephalitis and decreased bone size (Alizarin red staining) were noticed. BTV-1 antigen was first time demonstrable in cells of mesometrium, decidua of embryos, placenta, uterus, ovary, and brain of foetuses by immunohistochemistry and quantified by real-time qRT-PCR. BTV-inoculated mice were seroconverted by 7 and 5 dpi, and reached peak levels by 15 and 9 dpi in early and mid gestation, respectively. CD4+ and CD8+ cells were significantly decreased (increased ratio) on 7 dpi but subsequently increased on 15 dpi in early gestation. In mid gestation, increased CD8+ cells (decreased ratio) were observed. Apoptotic cells in PBMCs and tissues increased during peak viral load. This first time TPT of wild-type Indian BTV-1 deserves to be reported for implementation of control strategies. This model will be very suitable for further research into mechanisms of TPT, overwintering, and vaccination strategies

Molecular-genetic characterization of human parvovirus B19 prevalent in Kerala State, India

peer-reviewedBackground Human parvovirus B19V is a DNA virus, and a member of the family Parvoviridae, that causes various clinical manifestations, from asymptomatic to persistent infection that is associated with different autoimmune diseases. The parvovirus B19 evolves with a very high mutation rate that is closer to those of existing RNA viruses. Globally circulating B19V is currently classified into three genotypes, but their distribution is not spatially and temporally correlated. Except for a few recent reports on B19V entry into the human host and its genetic diversity, there is a lack of sufficient studies on this virus from distinct geographical locations and no clear understanding of its evolution has been documented. Methods To better understand the evolution of the Human parvo B19V virus from India's southern part, a geographically distinct location with no reports of B19V genomes, we have screened for B19V in 456 suspected cases using VP1/2 surface marker genes, and its characteristics were studied in detail. Amongst 456 clinically suspected B19V samples, 7.2% (33/456) were found positive by nested PCR (nPCR) were subsequently validated by real-time PCR, Sanger sequencing, and metagenome analysis. Results Human parvovirus B19 infection was shown among 33 of 456 patients when tested by nPCR; 30 among these were also positive by qPCR and were subsequently confirmed by sequencing 75% nPCR positive samples and 76% qPCR positive samples were from patients with age. ≥ 50 years respectively (Additional file 1: Table S1). The complete VP1/2 gene assembly from the South Indian strain showed three novel mutations (T122A, V128I, I283V), which might significantly impact the stability and virulence of the B19V virus circulating in this part of the world. These mutations might be crucial for its adaptive evolutionary strategies facilitating the spread and infectivity potential of the virus. In maximum likelihood phylogeny of VP1/2 sequences, the South Indian B19V strain forms a separate clade closer to the existing genotype two strains circulating worldwide. Conclusion Our study contributes to a better understanding of the human parvovirus's genetic and evolutionary characteristics in South India. Also, it highlights the possibility that a positive selection pressure acting on VP1/2 could increase the survival and replication capabilities of the viruses

Considerations of growth factor and material use in bone tissue engineering using biodegradable scaffolds in vitro and in vivo

Bone tissue engineering aims to harness materials to develop functional bone tissue to heal ‘critical-sized’ bone defects. This study examined a robust, coated poly(caprolactone) trimethacrylate (PCL-TMA) 3D-printable scaffold designed to augment bone formation. Following optimisation of the coatings, three bioactive coatings were examined, i) elastin-like polypeptide (ELP), ii) poly(ethyl acrylate) (PEA), fibronectin (FN) and bone morphogenetic protein-2 (BMP-2) applied sequentially (PEA/FN/BMP-2) and iii) both ELP and PEA/FN/BMP-2 coatings applied concurrently. The scaffold material was robust and showed biodegradability. The coatings demonstrated a significant (p < 0.05) osteogenic response in vitro in alkaline phosphatase gene upregulation and alkaline phosphatase production. The PCL-TMA scaffold and coatings supported angiogenesis and displayed excellent biocompatibility following evaluation on the chorioallantoic membrane assay. No significant (p < 0.05) heterotopic bone formed on the scaffolds within a murine subcutaneous implantation model, compared to the positive control of BMP-2 loaded collagen sponge following examination by micro-computed tomography or histology. The current studies demonstrate a range of innovative coated scaffold constructs with in vitro efficacy and clearly illustrate the importance of an appropriate in vivo environment to validate in vitro functionality prior to scale up and preclinical application

Conservation of seagrass beds with special reference to associated species and fishery resources

The southeast coast of India bordering Gulf of Mannar and Palk Bay has luxuriant growth of seagrass beds which sustain good fisheries and is a biodiversity hotspot. Many fishers living along the coast depend on these resources for their livelihood. Several species of invertebrates and vertebrates fully depend on the seagrass resources for their survival and sustenance. In this paper, the diversity of species commonly associated with the seagrass beds and their present status is presented followed by the fisheries associated with this ecosystem. The type of fishing activities including the details of crafts, gears, number of fishers involved and catch statistics are presented. The main objective of this approach is to understand how the fishing activities influence the ecosystem and fishermen. Underwater monitoring and visual observations were documented through photographs and videos. Artisanal fishing activities such as bottom set gillnet, push net, lead-fish trap net and shore seine are in detail discussed and the conservation measures which are urgently required are also presented

Influence of selected anthropogenic impacts on ecology of Vembanad Lake

Influence of selected anthropogenic impacts on ecology of Vembanad Lak

Nanotopography reveals metabolites that maintain the immunomodulatory phenotype of mesenchymal stromal cells

Mesenchymal stromal cells (MSCs) are multipotent progenitor cells that are of considerable clinical potential in transplantation and anti-inflammatory therapies due to their capacity for tissue repair and immunomodulation. However, MSCs rapidly differentiate once in culture, making their large-scale expansion for use in immunomodulatory therapies challenging. Although the differentiation mechanisms of MSCs have been extensively investigated using materials, little is known about how materials can influence paracrine activities of MSCs. Here, we show that nanotopography can control the immunomodulatory capacity of MSCs through decreased intracellular tension and increasing oxidative glycolysis. We use nanotopography to identify bioactive metabolites that modulate intracellular tension, growth and immunomodulatory phenotype of MSCs in standard culture and during larger scale cell manufacture. Our findings demonstrate an effective route to support large-scale expansion of functional MSCs for therapeutic purposes