Seed germination of four amaranth species (Amaranthus spp.)

Amaranth (Amarantus spp.) is an important species for its benefits in human and animal nutrition, therefore, the reproduction of these species is important, supported by rigorous experiments that generate quality information. Consequently, the present work aimed to determine the germination of 20 accessions of four species of Amaranthus spp. This was carried out at the Technological Institute of Chiná, Campeche, Mexico, with seeds donated by The North Central Regional Plant Introduction Station (NCRPIS), Iowa State University, from Africa, Asia, Greece, South America, USA, and Mexico. The seeds were weighed and measured, then they were sown in Petri dishes and kept in a germination chamber at 27 ºC and a relative humidity of 54 % in the dark, every 24 hours the germinated seeds were counted and removed. The analyzes were performed using the ANOVA test to identify differences in weight, length and germination between accessions. The results did not show statistical differences in seed length, between species or between accessions, with respect to weight if there were statistical differences both between species and between accessions. The results showed that A. hypochondriacus from India had the highest weight (0.00093 ± 0.000075 g). Germination did obtain statistical differences in the different evaluation times, at 24 and 48 hours, where the highest germination was in the species A. hypochondriacus and A cruentus. Therefore, it is concluded that seed germination is different in each species and accession.Amaranthus (Amaranthus spp.) is a species of great importance that benefits human and animal nutrition; therefore, its reproduction must be based on useful information obtained from rigorous experiments. Consequently, the aim of this work was to determine the germination of 20 accessions from four Amaranthus spp. The work was carried out at the Instituto Tecnológico de Chiná, Campeche, Mexico, using seeds from Africa, Asia, Greece, South America, the US, and Mexico, donated by The North Central Regional Plant Introduction Station (NCRPIS), Iowa State University. After they were weighed and measured, the seeds were placed in Petri dishes and kept in the dark inside a germination chamber, at 27 ºC and with a 54% relative humidity. Germinated seeds were counted and removed every 24 hours. The analyses were carried out using the ANOVA test in order to identify weight, length, and germination differences between accessions. The results showed no statistical differences in seed length, neither between species nor accessions; however, there were statistical differences in the weight, both between species and between accessions. A. hypochondriacus from India recorded the highest weight (0.00093 ± 0.000075 g). Regarding germination, there were statistical differences between the various evaluation periods (24 and 48 hours): the highest germination was recorded by A. hypochondriacus and A cruentus. Therefore, the following conclusion was reached: seed germination is different between species and accessions

Evaluation of a quality improvement intervention to reduce anastomotic leak following right colectomy (EAGLE): pragmatic, batched stepped-wedge, cluster-randomized trial in 64 countries

Background Anastomotic leak affects 8 per cent of patients after right colectomy with a 10-fold increased risk of postoperative death. The EAGLE study aimed to develop and test whether an international, standardized quality improvement intervention could reduce anastomotic leaks. Methods The internationally intended protocol, iteratively co-developed by a multistage Delphi process, comprised an online educational module introducing risk stratification, an intraoperative checklist, and harmonized surgical techniques. Clusters (hospital teams) were randomized to one of three arms with varied sequences of intervention/data collection by a derived stepped-wedge batch design (at least 18 hospital teams per batch). Patients were blinded to the study allocation. Low- and middle-income country enrolment was encouraged. The primary outcome (assessed by intention to treat) was anastomotic leak rate, and subgroup analyses by module completion (at least 80 per cent of surgeons, high engagement; less than 50 per cent, low engagement) were preplanned. Results A total 355 hospital teams registered, with 332 from 64 countries (39.2 per cent low and middle income) included in the final analysis. The online modules were completed by half of the surgeons (2143 of 4411). The primary analysis included 3039 of the 3268 patients recruited (206 patients had no anastomosis and 23 were lost to follow-up), with anastomotic leaks arising before and after the intervention in 10.1 and 9.6 per cent respectively (adjusted OR 0.87, 95 per cent c.i. 0.59 to 1.30; P = 0.498). The proportion of surgeons completing the educational modules was an influence: the leak rate decreased from 12.2 per cent (61 of 500) before intervention to 5.1 per cent (24 of 473) after intervention in high-engagement centres (adjusted OR 0.36, 0.20 to 0.64; P < 0.001), but this was not observed in low-engagement hospitals (8.3 per cent (59 of 714) and 13.8 per cent (61 of 443) respectively; adjusted OR 2.09, 1.31 to 3.31). Conclusion Completion of globally available digital training by engaged teams can alter anastomotic leak rates. Registration number: NCT04270721 (http://www.clinicaltrials.gov)