9 research outputs found
Pyrrole-based photoswitchable anion recepotrs
The main goal of this thesis is to achieve advanced control of binding properties in bis-pyrrolic and macrocyclic pyrrolic receptors by light. Pyrrole-based receptors, and in particular calix[4]pyrroles are excellent anion receptors, but only few examples have been reported in which they were modified with photoresponsive units. In this thesis, stiff-stilbene is first equipped with amidopyrrole units (chapter 2) and then used as linker in strapped calix[4]pyrrole (chapter 3 and 4). Alternatively, dithienylethene has been incorporated into strapped calix[4]pyrrole with the goal of changing binding selectivity in addition to affinity (chapter 5). Finally, the drawback of using UV light is addressed by red-shifting the excitation wavelength of stiff-stilbene (chapter 6).Supramolecular & Biomaterials Chemistr
StiffâStilbene Photoswitches: From Fundamental Studies to Emergent Applications
Stiffâstilbene, a sterically restricted fused ring analogue of stilbene, has been regularly used as a model compound in theoretical studies of stilbene photoisomerization. Lately, owing to its excellent photoswitching properties, it is increasingly being applied to reversibly control the properties and function of chemical as well as biological systems. Stiffâstilbene photoswitches possess a number of advantageous properties including a high quantum yield for photoisomerization and a high thermal stability. Furthermore, they undergo a large geometrical change upon isomerization and their synthesis is straightforward. Herein, we provide an overview of the basic properties of stiffâstilbene and of recent applications in supramolecular chemistry, catalysis, and biological systems.Supramolecular & Biomaterials Chemistr
Pushâpull stiffâstilbene: protonâgated visibleâlight photoswitching and acidâcatalyzed isomerization
Supramolecular & Biomaterials Chemistr
Photoswitching of halide-binding affinity and selectivity in dithienylethene-strapped calix[4]pyrrole
Supramolecular & Biomaterials Chemistr
Intra-articular injection of two different doses of autologous bone marrow mesenchymal stem cells versus hyaluronic acid in the treatment of knee osteoarthritis: long-term follow up of a multicenter randomized controlled clinical trial (phase I/II)
Background: Mesenchymal stromal cells (MSCs) are a promising option to treat knee osteoarthritis (OA). Their safety
and usefulness have been reported in several short-term clinical trials but less information is available on the longterm efects of MSC in patients with osteoarthritis. We have evaluated patients included in our previous randomized
clinical trial (CMM-ART, NCT02123368) to determine their long-term clinical efect.
Materials: A phase I/II multicenter randomized clinical trial with active control was conducted between 2012 and
2014. Thirty patients diagnosed with knee OA were randomly assigned to Control group, intraarticularly administered
hyaluronic acid alone, or to two treatment groups, hyaluronic acid together with 10Ă106
or 100Ă106
cultured autolâ
ogous bone marrow-derived MSCs (BM-MSCs), and followed up for 12 months. After a follow up of 4 years adverse
efects and clinical evolution, assessed using VAS and WOMAC scorings are reported.
Results: No adverse efects were reported after BM-MSCs administration or during the follow-up. BM-MSCs-adminisâ
tered patients improved according to VAS, median value (IQR) for Control, Low-dose and High-dose groups changed
from 5 (3, 7), 7 (5, 8) and 6 (4, 8) to 7 (6, 7), 2 (2, 5) and 3 (3, 4), respectively at the end of follow up (Low-dose vs Control
group, p=0.01; High-dose vs Control group, p=0.004). Patients receiving BM-MSCs also improved clinically accordâ
ing to WOMAC. Control group showed an increase median value of 4 points (â11;10) while Low-dose and Highdose groups exhibited values of â18 (â28;â9) and â10 (â21;â3) points, respectively (Low-dose vs Control group
p=0.043). No clinical diferences between the BM-MSCs receiving groups were found.
Conclusions: Single intraarticular injection of in vitro expanded autologous BM-MSCs is a safe and feasible proceâ
dure that results in long-term clinical and functional improvement of knee OA
Photoswitchable bis(amidopyrroles): modulating anion transport activity independent of binding affinity
Supramolecular & Biomaterials Chemistr
Intra-articular injection of two different doses of autologous bone marrow mesenchymal stem cells versus hyaluronic acid in the treatment of knee osteoarthritis: long-term follow up of a multicenter randomized controlled clinical trial (phase I/II)
Background: Mesenchymal stromal cells (MSCs) are a promising option to treat knee osteoarthritis (OA). Their safety
and usefulness have been reported in several short-term clinical trials but less information is available on the longterm efects of MSC in patients with osteoarthritis. We have evaluated patients included in our previous randomized
clinical trial (CMM-ART, NCT02123368) to determine their long-term clinical efect.
Materials: A phase I/II multicenter randomized clinical trial with active control was conducted between 2012 and
2014. Thirty patients diagnosed with knee OA were randomly assigned to Control group, intraarticularly administered
hyaluronic acid alone, or to two treatment groups, hyaluronic acid together with 10Ă106
or 100Ă106
cultured autolâ
ogous bone marrow-derived MSCs (BM-MSCs), and followed up for 12 months. After a follow up of 4 years adverse
efects and clinical evolution, assessed using VAS and WOMAC scorings are reported.
Results: No adverse efects were reported after BM-MSCs administration or during the follow-up. BM-MSCs-adminisâ
tered patients improved according to VAS, median value (IQR) for Control, Low-dose and High-dose groups changed
from 5 (3, 7), 7 (5, 8) and 6 (4, 8) to 7 (6, 7), 2 (2, 5) and 3 (3, 4), respectively at the end of follow up (Low-dose vs Control
group, p=0.01; High-dose vs Control group, p=0.004). Patients receiving BM-MSCs also improved clinically accordâ
ing to WOMAC. Control group showed an increase median value of 4 points (â11;10) while Low-dose and Highdose groups exhibited values of â18 (â28;â9) and â10 (â21;â3) points, respectively (Low-dose vs Control group
p=0.043). No clinical diferences between the BM-MSCs receiving groups were found.
Conclusions: Single intraarticular injection of in vitro expanded autologous BM-MSCs is a safe and feasible proceâ
dure that results in long-term clinical and functional improvement of knee OA