Constraint Based Hybrid Approach to Parsing Indian Languages

PACLIC 23 / City University of Hong Kong / 3-5 December 200

Alkaline lipase production by <i style="">Citrobacter freundii</i> IIT-BT L139

485-491Around 150 lipase producing bacterial isolates were screened from the local soils enriched with oil. Citrobacter freundii IIT-BT L139, an isolated microbial strain, produced lipase that had high activity (8.8 U/ml) at pH 9.0 and 40oC. The 16S rDNA phylogenetic studies showed that Citrobacter freundii belongs to the family Enterobacteriaceae and later confirmed by the microbial identification. Suitable C and N sources for lipase production were deduced to be starch and peptone-urea, respectively. In a controlled fermenter (1 L), the lipase activity was found to increase by 36% (12 Uml-1). The variation of lipase activity, pH and dissolved oxygen (DO) during growth of the organism in the controlled batch fermenter were monitored. The rheological characteristics of the fermentation broth indicated that it behaved like a Newtonian fluid throughout the fermentation. The fermentation time was comparatively short (60 h). The lipase was also found to be substantially resistant to common detergents. This lipase was, thus, characterized as alkaline, thermostable and solvent stable, which was essentially desirable in pharmaceutical, detergent and other industrial applications or production

Salicylic acid-induced glutathione status in tomato crop and resistance to root-knot nematode, Meloidogyne incognita (Kofoid & White) Chitwood

Salicylic acid-(SA) is a plant defense stimulator. Exogenous application of SA might influence the status of glutathione-(GSH). GSH activates and SA alters the expression of defense genes to modulate plant resistance against pathogens. The fate of GSH in a crop following SA treatment is largely unknown. The SA-induced profiles of free reduced-, free oxidized-(GSSG) and protein bound-(PSSG) glutathione in tomato crop following foliar treatment of transplant at 5.0-10.0 &mu;g mL&ndash;1 were measured by liquid chromatography. Resistance to root-knot nematode, Meloidogyne incognita damaging tomato and crop performance were also evaluated. SA treatment at 5.0-10.0 &mu;g mL&ndash;1 to tomato transplants increased GSH, GSSG and PSSG in plant leaf and root, more so in leaf, during crop growth and development. As the fruits ripened, GSH and PSSG increased and GSSG declined. SA reduced the root infection by M. incognita, nematode reproduction and thus, improved the resistance of tomato var. Pusa Ruby, but reduced crop growth and redox status. SA at 5.0 &mu;g mL&ndash;1 improved yield and fruit quality. The study firstly linked SA with activation of glutathione metabolism and provided an additional dimension to the mechanism of induced resistance against obligate nematode pathogen. SA increased glutathione status in tomato crop, imparted resistance against M. incognita, augmented crop yield and functional food quality. SA can be applied at 5.0 &mu;g mL&ndash;1 for metabolic engineering of tomato at transplanting to combine host-plant resistance and health benefits in formulating a strategic nematode management decision

Platform Tilt Detection : For Drone Landing

This report describes a project aimed at determining the angle of the landing platform to the drones using a tilted platform, IMU, and image processing algorithms. The goal of this project is to determine the safe landing of drones and drone landings by optimizing the landing location via platform tilt adjustment and angle computation. A robotic arm was used to tilt the rectangular platform, and a web camera was used to get images from the top view. Image processing methods built-in MATLAB allow for approximate landing angle determination and angle determination is also done with IMU. The findings confirm the system’s efficiency, with reasonable angle estimates and successful drone landings

Platform Tilt Detection : For Drone Landing

This report describes a project aimed at determining the angle of the landing platform to the drones using a tilted platform, IMU, and image processing algorithms. The goal of this project is to determine the safe landing of drones and drone landings by optimizing the landing location via platform tilt adjustment and angle computation. A robotic arm was used to tilt the rectangular platform, and a web camera was used to get images from the top view. Image processing methods built-in MATLAB allow for approximate landing angle determination and angle determination is also done with IMU. The findings confirm the system’s efficiency, with reasonable angle estimates and successful drone landings

Development and validation of novel stability indicating RP-HPLC method for the determination of assay of voriconazole in pharmaceutical products

For the determination of the assay of voriconazole in bulk and in pharmaceutical dosage forms, a novel stability indicating RP-HPLC method was designed and validated, exhibiting a very low run time. The stability-indicating nature of the approach is supported by the fact that it is unique, quick, precise, accurate, and capable of isolating the voriconazole peak from any contaminating or degrading components. Isocratic elution on a 100 mm x 4.6 mm, 3μm agilent C18 column at 45°C and a UV detection wavelength of 256 nm constitutes the analytical procedure at at a flow rate of 1.0 mL/min. After injecting 20µL of voriconazole sample, the elution peak occurred at 3.5 minutes, and the entire run time was 15 minutes. Between 98% and 102% was a reasonable range for the percentage of recovery. It was determined that the method's RSD for precision and accuracy was less than 2%. The method has been verified for routine analysis of voriconazole in bulk materials and its formulations according to the standards established by the International Conference on Harmonization (ICH)

Development and validation of novel stability indicating RP-HPLC method for the determination of assay of voriconazole in pharmaceutical products

For the determination of the assay of voriconazole in bulk and in pharmaceutical dosage forms, a novel stability indicating RP-HPLC method was designed and validated, exhibiting a very low run time. The stability-indicating nature of the approach is supported by the fact that it is unique, quick, precise, accurate, and capable of isolating the voriconazole peak from any contaminating or degrading components. Isocratic elution on a 100 mm x 4.6 mm, 3μm agilent C18 column at 45°C and a UV detection wavelength of 256 nm constitutes the analytical procedure at at a flow rate of 1.0 mL/min. After injecting 20µL of voriconazole sample, the elution peak occurred at 3.5 minutes, and the entire run time was 15 minutes. Between 98% and 102% was a reasonable range for the percentage of recovery. It was determined that the method's RSD for precision and accuracy was less than 2%. The method has been verified for routine analysis of voriconazole in bulk materials and its formulations according to the standards established by the International Conference on Harmonization (ICH)