Natural Magnetite Minerals Enhance 1,2-Dichloroethane Reductive Dechlorination

Contamination of soil and groundwater by chlorinated solvents is an environmental issue of primary concern. Recently, electrically conductive iron particles have been proposed as a novel approach to accelerate anaerobic bioremediation processes. In fact, it was demonstrated that conductive particles facilitate the exchange of electrons between microorganisms via Direct Interspecies Electron Transfer (DIET) processes, thus enhancing the pollutant-degrading potential of the microbial community. However, the use of natural minerals in this context has not been reported so far. In this study, we applied, for the first time, natural magnetite and hematite to accelerate the reductive dechlorination of 1,2-dichloroethane by an enrichment culture in lab-scale anaerobic microcosms. After four feeding cycles, low magnetite-amended microcosms (13 mg/L) yielded the highest rate of 1,2-DCA reductive dechlorination and reduced methanogenic activity. By contrast, hematite did not display any apparent stimulatory effect. Surprisingly, in the presence of higher amounts of iron oxides, a weaker effect was obtained, probably because iron(III) present in the minerals competed for the electrons necessary for reductive dechlorination. For all microcosms, the concentration of the toxic byproduct vinyl chloride was negligible throughout the whole study. The SEM/EDS analysis confirmed the close interaction between the conductive iron oxide particles and the dechlorinating bacteria. This work opens the possibility of using natural conductive minerals for bioremediation applications as well as shedding light on the previously unrecognized role of such minerals in contaminated ecosystemsThe authors would like to thank FCT (Portuguese Foundation for Science and Technology) for the financial support of Patrícia Leitão through the Ph.D. grant SFRH/BD/87312/2012info:eu-repo/semantics/publishedVersio

Enhancing methane production from food waste fermentate using biochar. The added value of electrochemical testing in pre-selecting the most effective type of biochar

Background: Recent studies have suggested that addition of electrically conductive biochar particles is an effective strategy to improve the methanogenic conversion of waste organic substrates, by promoting syntrophic associations between acetogenic and methanogenic organisms based on interspecies electron transfer processes. However, the underlying fundamentals of the process are still largely speculative and, therefore, a priori identification, screening, and even design of suitable biochar materials for a given biotechnological process are not yet possible. Results: Here, three charcoal-like products (i.e., biochars) obtained from the pyrolysis of different lignocellulosic materials, (i.e., wheat bran pellets, coppiced woodlands, and orchard pruning) were tested for their capacity to enhance methane production from a food waste fermentate. In all biochar-supplemented (25 g/L) batch experiments, the complete methanogenic conversion of fermentate volatile fatty acids proceeded at a rate that was up to 5 times higher than that observed in the unamended (or sand-supplemented) controls. Fluorescent in situ hybridization analysis coupled with confocal laser scanning microscopy revealed an intimate association between archaea and bacteria around the biochar particles and provided a clear indication that biochar also shaped the composition of the microbial consortium. Based on the application of a suite of physico-chemical and electrochemical characterization techniques, we demonstrated that the positive effect of biochar is directly related to the electron-donating capacity (EDC) of the material, but is independent of its bulk electrical conductivity and specific surface area. The latter properties were all previously hypothesized to play a major role in the biochar-mediated interspecies electron transfer process in methanogenic consortia. Conclusions: Collectively, these results of this study suggest that for biochar addition in anaerobic digester operation, the screening and identification of the most suitable biochar material should be based on EDC determination, via simple electrochemical tests. © 2017 The Author(s)

An underappreciated DIET for anaerobic petroleum hydrocarbon‐degrading microbial communities

Direct interspecies electron transfer (DIET) via electrically conductive minerals can play a role in the anaerobic oxidation of petroleum hydrocarbons in contaminated sites and can be exploited for the development of new, more effective bioremediation approaches

Removal of Hepatitis B virus surface HBsAg and core HBcAg antigens using microbial fuel cells producing electricity from human urine

© 2019, The Author(s). Microbial electrochemical technology is emerging as an alternative way of treating waste and converting this directly to electricity. Intensive research on these systems is ongoing but it currently lacks the evaluation of possible environmental transmission of enteric viruses originating from the waste stream. In this study, for the first time we investigated this aspect by assessing the removal efficiency of hepatitis B core and surface antigens in cascades of continuous flow microbial fuel cells. The log-reduction (LR) of surface antigen (HBsAg) reached a maximum value of 1.86 ± 0.20 (98.6% reduction), which was similar to the open circuit control and degraded regardless of the recorded current. Core antigen (HBcAg) was much more resistant to treatment and the maximal LR was equal to 0.229 ± 0.028 (41.0% reduction). The highest LR rate observed for HBsAg was 4.66 ± 0.19 h−1 and for HBcAg 0.10 ± 0.01 h−1. Regression analysis revealed correlation between hydraulic retention time, power and redox potential on inactivation efficiency, also indicating electroactive behaviour of biofilm in open circuit control through the snorkel-effect. The results indicate that microbial electrochemical technologies may be successfully applied to reduce the risk of environmental transmission of hepatitis B virus but also open up the possibility of testing other viruses for wider implementation

Sulphate Reduction Processes in Biological Permeable Reactive Barriers: Column Experimentation and Modeling

In this work a mathematical model for simulating biological sulphate reduction processes was developed and validated by experimental data in fixed bed columns as lab-scale representations of permeable reactive barriers (PRBs). The model takes in consideration transport and adsorption of sulphate and heavy metals, sulphate bioreduction, bioprecipitation and chemical precipitation of metals. Firstly, the effect of sorption capacity of solid phase of column filling versus the effect of active biological mechanisms on solute removal was isolated denoting the significant contribution of sorption versus bioprecipitation of heavy metals. Subsequently, the mathematical model was validated using experimental data from laboratory column experiments and a good agreement between experimental data and simulation results was obtained. Sensitivity analysis of dynamic model showed that, after steady state was reached, parameter related to biological sulphate reduction affected the model output in the most significant way

Development of new composite biosorbents from olive pomace wastes

In this study olive pomace was used as a source of binding substances for the development of composite biosorbents to be used in heavy metal removal from aqueous solutions. The aim was to obtain biosorbent material with an increased concentration of binding sites. The effects of two different extraction procedures (one using only methanol and the other one hexane followed by methanol) on the binding properties of olive pomace were tested by potentiometric titrations and batch biosorption tests for copper and cadmium removal. Titration modelling evidenced that both kinds of extractions generated a solid with a reduced amount of protonatable sites. Biosorption tests were organized according to full factorial designs. Analysis of variance denoted that both kinds of extractions determined a statistically significant negative effect on metal biosorption. In the case of cadmium extractions also determined a significant decrease of selectivity with respect to olive pomace. When the acid-base and binding properties of the substances extracted were determined, they were adsorbed onto a synthetic resin (octadecylsilane) and calcium alginate beads. In this way two kinds of composite biosorbents have been obtained both having an increased concentration of binding substances with respect to native olive pomace, also working more efficiently in metal removal. (C) 2010 Elsevier B. V. All rights reserved

Isolation and quantification of cadmium removal mechanisms in batch reactors inoculated by sulphate reducing bacteria: Biosorption versus bioprecipitation

Biosorbing properties Of sulphate reducing bacteria were tested to distinguish the amount of cadmium removed by bioprecipitation from that bound onto biomass Surface (biosorption). Experimental results of cadmium abatement in batch growth tests (bioprecipitation tests) were then compared with metabolism-independent binding properties of SRB cell wall Surface (biosorption tests performed with dead biomass). Experimental results showed that SRB inoculum removed 59 +/- 5% of sulphates in 21 days even in presence of cadmium (0-36 mmol L(-1)), while non-monotonous kinetic effects were observed for increasing Cd concentrations. Comparison between bioprecipitation and biosorption tests denoted a significant contribution of biosorption (77%) in total Cd removal (0.40 +/- 0.01 mmol g(-1)). Characterisation of bacterial acid-base surface properties by potentiometric titrations and mechanistic modelling denoted that carboxylic, phosphate and amino groups of cell wall are the main responsible of metal removal by biosorption mechanism. (C) 2009 Elsevier Ltd. All rights reserved