663 research outputs found

    Enhanced resistance to citrus canker in transgenic sweet orange expressing the sarcotoxin IA gene.

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    Citrus canker, caused by the bacterial pathogen Xanthomonas citri subp. Citri (Xcc), is a serious disease reported in most citrus-producing areas around the world. Although different levels of field resistance to citrus canker have been reported in sweet oranges, they are usually not sufficient to provide adequate control of the disease. Ectopic over-expression of antibacterial genes is one of the potential strategies to increase plant resistance to bacterial diseases. Previous in vitro results showed that sarcotoxin IA, an antimicrobial peptide isolated from the flesh fly (Sarcophaga peregrina), can be efficient to control different plant pathogenic bacteria, including Xcc. Transgenic BPera^ sweet orange (Citrus sinensis [L.] Osbeck) plants constitutively expressing the sarcotoxin IA peptide fused to the PR1a signal peptide from Nicotiana tabacum for secretion in the intercellular space were obtained by Agrobacteriummediated transformation using thin sections of mature explants. Citrus canker resistance evaluation in leaves of transgenic and non-transgenic plants was performed through inoculations with Xcc by infiltration and spraying. The Xcc population was up to 2 log unit lower in leaves of the transgenic plants compared to those of non-transgenic controls. Incidence of canker lesions was significantly higher in non-transformed controls (>10 lesions/cm2) than in the transgenic plants (<5 lesions/ cm2) after injection infiltration or spraying with Xcc inoculum. Accumulation of sarcotoxin IA peptide in sweet orange tissue did not cause any deleterious effects on the growth and development of the transgenic plants, indicating this approach is suitable to provide resistance to citrus canker

    can a rapid local cooling intervention help young soccer players?

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    The effects of a cooling strategy following repeated high-intensity running (RHIR) on soccer kicking performance in a hot environment (>30ºC) were investigated in youth soccer players. Fifteen academy under-17 players participated. In Experiment 1, players completed an all-out RHIR protocol (10×30 m, with 30s intervals). In Experiment 2 (cross-over design), participants performed this running protocol under two conditions: (1) following RHIR 5 minutes of cooling where ice packs were applied to the quadriceps/hamstrings, (2) a control condition involving passive resting. Perceptual measures [ratings of perceived exertion (RPE), pain and recovery], thigh temperature and kick-derived video three-dimensional kinematics (lower limb) and performance (ball speed and two-dimensional placement indices) were collected at baseline, post-exercise and intervention. In Experiment 1, RHIR led to small- to-large impairments (p < 0.03;d = −0.42–-1.83) across perceptual, kinematic and performance measures. In experiment 2, RPE (p < 0.01; Kendall’s W = 0.30) and mean radial error (p = 0.057; η2 = 0.234) increased only post-control. Significant small declines in ball speed were also observed post-control (p < 0.05; d = 0.35). Post-intervention foot centre-of-mass velocity was moderately faster in the cooling compared to control condition (p = 0.04; d = 0.60). In youth soccer players, a short cooling period was beneficial in counteracting declines in kicking performance, in particular ball placement, following intense running activity in the heat.9E1A-F9DD-3EB8 | Filipe Manuel ClementeN/

    Estimativa de parâmetros genéticos em famílias C1So:3 de seleção recorrente para produtividade em feijoeiro comum de grão preto.

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    Os objetivos desse trabalho foram estimar parâmetros genéticos e avaliar famílias C1So:3 do programa de seleção recorrente de feijoeiro comum preto da Embrapa Arroz e Feijão, visando à seleção de famílias superiores para obtenção de linhagens e intercruzamento para formação da nova população de seleção

    Estimativa de parâmetros genéticos em famílias C1So: 3 de seleção recorrente para produtividade em feijoeiro comum de grãos carioca.

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    Os objetivos desse trabalho foram estimar parâmetros genéticos e avaliar famílias C1So:3 do programa de seleção recorrente da Embrapa Arroz e Feijão de grão carioca, visando a seleção de famílias superiores para obtenção de linhagens e intercruzamento para formação da nova população de seleção

    Oxidação lipídica em filés de tambaqui (Colossoma macropomum) defumados com alecrim (Rosmarinus officinalis).

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