BizWeb: Kreuzfahrtbuchungen mit Web Services - Projektdokumentation

Das Anwendungsziel war, mit Hilfe von Web Services, eine Kreuzfahrtbuchung in einem SAPSystem zu realisieren. Damit soll die Abfrage nach buchbaren Kreuzfahrten, die Buchung von Kreuzfahrten usw. in einem fiktiven Kreuzfahrtbuchungssystem über ein SAPSystem ermöglicht werden. Hierzu war eine Schnittstelle auf der Basis des Java Connectors zu realisieren. Dazu waren vielfältige Vorarbeiten notwendig, wie: ein Kreuzfahrtbuchungssystem im SAPSystem zu realisieren, das notwendige Datenmodell zu entwickeln, etc

Tailor-made resealable micro(bio)reactors providing easy integration of in situ sensors

Viefhues M, Sun S, Valikhani D, et al. Tailor-made resealable micro(bio)reactors providing easy integration of in situ sensors. Journal of Micromechanics and Microengineering. 2017;27(6): 065012

A Spring in Performance: Silica Nanosprings Boost Enzyme Immobilization in Microfluidic Channels

Enzyme microreactors are important tools of miniaturized analytics and have promising applications in continuous biomanufacturing. A fundamental problem of their design is that plain microchannels without extensive static internals, or packings, offer limited exposed surface area for immobilizing the enzyme. To boost the immobilization in a manner broadly applicable to enzymes, we coated borosilicate microchannels with silica nanosprings and attached the enzyme, sucrose phosphorylase, via a silica-binding module genetically fused to it. We showed with confocal fluorescence microscopy that the enzyme was able to penetrate the ∼70 μm-thick nanospring layer and became distributed uniformly in it. Compared with the plain surface, the activity of immobilized enzyme was enhanced 4.5-fold upon surface coating with nanosprings and further increased up to 10-fold by modifying the surface of the nanosprings with sulfonate groups. Operational stability during continuous-flow biocatalytic synthesis of α-glucose 1-phosphate was improved by a factor of 11 when the microreactor coated with nanosprings was used. More than 85% of the initial conversion rate was retained after 840 reactor cycles performed with a single loading of enzyme. By varying the substrate flow rate, the microreactor performance was conveniently switched between steady states of quantitative product yield (50 mM) and optimum productivity (19 mM min^–1) at a lower product yield of 40%. Surface coating with silica nanosprings thus extends the possibilities for enzyme immobilization in microchannels. It effectively boosts the biocatalytic function of a microstructured reactor limited otherwise by the solid surface available for immobilizing the enzyme

Analytics in Microfluidic Systems

Viefhues M. Analytics in Microfluidic Systems. In: Advances in Biochemical Engineering/Biotechnology. Berlin ; Heidelberg: Springer ; 2020.Microfluidic analysis proved to be very sufficient in supporting biotechnological studies. This is due to the wide range of new analysis methods that provide further insight into biotechnological questions but also to intrinsic advantages of the systems themselves. To name two of them, only very small sample amounts are needed, and the analytics are very fast. In this overview paper, microfluidic analysis methods are introduced with a special focus on electric analysis methods. The aim of this work is to shed light on the special advantages of miniaturized electrical analysis in microfluidics; the main theoretical aspects of the methods are given together with the potential scientific insight that can be gained by the respective methods