Older Adults’ Deployment of ‘Distrust’

Older adults frequently deploy the concept of distrust when discussing digital technologies, and it is tempting to assume that distrust is largely responsible for the reduced uptake by older adults witnessed in the latest surveys of technology use. To help understand the impact of distrust on adoption behavior, we conducted focus groups with older adults exploring how, in what circumstances, and to what effect older adults articulate distrust in digital technologies. Our findings indicate that distrust is not especially relevant to older adults’ practical decision making around technology (non-)use. The older adults in our study used the language of distrust to open up discussions around digital technologies to larger issues related to values. This suggests that looking to distrust as a predictor of non-use (e.g. in Technology Acceptance Model studies) may be uniquely unhelpful in the case of older adults, as it narrows the discussion of technology acceptance and trust to interactional issues, when their use of distrust pertains to much wider concerns. Likewise, technology adoption should not be viewed as indicative of trust or an endorsement of technology acceptability. Older adults using-while-distrusting offers important insights into how to design truly acceptable digital technologies

Purification of a novel pyruvate kinase from a green alga

AbstractA chloroplast pyruvate kinase has been purified to homogeneity from the green alga Selenastrum minutum. The subunit composition of the enzyme differs markedly from pyruvate kinases from other sources. Subunit and native Mrs were 210 kDa and 235 kDa, respectively, indicating a monomeric structure. Immunological and peptide mapping analyses suggested structural homology to a bacterial, but not higher plant or mammalian pyruvate kinase

Involving patients and carers in developing the radiotherapy curriculum: enhancing compassion

Background:: This article describes a collaborative project that aimed to develop a patient-centred curriculum in radiotherapy. In the wake of the Francis report in 2013 and a call for compassion to be a central tenet of health programmes, the project was a timely opportunity to enhance the radiotherapy curriculum. Methods:: Collaboration between university staff and patients and carers using the service improvement model Plan-Do-Study-Act was the method employed for the curriculum project. Two key discussion forums helped shape the curriculum plan, with module and course evaluation continuing to inform developments. Results:: The key outcome of the project is that it has shaped the 'care' theme evident in the current undergraduate programme. Co-production methods resulted in the development of a range of shared classroom activities that focus on experiences, care values and communication strategies. The new curriculum has evaluated positively and the impact of learning is demonstrated both in the classroom and clinical setting. The project team have also influenced recruitment processes and patient and carer involvement in programme approval is embedded. Conclusion:: Working together, with patients and carers is an ideal method to enhance the curriculum and reflect the requirements in practice of current health and social care professions. Further developments in student assessment are planned

The Wisdom of Older Technology (Non-)Users

Older adults consistently reject digital technology even when designed to be accessible and trustworthy

In vivo regulatory phosphorylation of the phosphoenolpyruvate carboxylase AtPPC1 in phosphate-starved Arabidopsis thaliana

PEPC [PEP(phosphoenolpyruvate) carboxylase] is a tightly controlled cytosolic enzyme situated at a major branchpoint in plant metabolism. Accumulating evidence indicates important functions for PEPC and PPCK (PEPC kinase) in plant acclimation to nutritional Pi deprivation. However, little is known about the genetic origin or phosphorylation status of native PEPCs from −Pi (Pi-deficient) plants. The transfer of Arabidopsis suspension cells or seedlings to −Pi growth media resulted in: (i) the marked transcriptional upregulation of genes encoding the PEPC isoenzyme AtPPC1 (Arabidopsis thaliana PEPC1), and PPCK isoenzymes AtPPCK1 and AtPPCK2; (ii) >2-fold increases in PEPC specific activity and in the amount of an immunoreactive 107-kDa PEPC polypeptide (p107); and (iii) In vivo p107 phosphorylation as revealed by immunoblotting of clarified extracts with phosphosite-specific antibodies to Ser-11 (which could be reversed following Pi resupply). Approx. 1.3 mg of PEPC was purified 660-fold from −Pi suspension cells to apparent homogeneity with a specific activity of 22.3 units · mg−1 of protein. Gel filtration, SDS/PAGE and immunoblotting demonstrated that purified PEPC exists as a 440-kDa homotetramer composed of identical p107 subunits. Sequencing of p107 tryptic and Asp-N peptides by tandem MS established that this PEPC is encoded by AtPPC1. Pi-affinity PAGE coupled with immunoblotting indicated stoichiometric phosphorylation of the p107 subunits of AtPPC1 at its conserved Ser-11 phosphorylation site. Phosphorylation activated AtPPC1 at pH 7.3 by lowering its Km(PEP) and its sensitivity to inhibition by L-malate and L-aspartate, while enhancing activation by glucose 6-phosphate. Our results indicate that the simultaneous induction and In vivo phosphorylation activation of AtPPC1 contribute to the metabolic adaptations of −Pi Arabidopsis

HCI and Aging:Beyond Accessibility

Despite improvements in the accessibility of digital technologies and growing numbers of tools designed specifically for older adults, adoption of such tools remains low for this demographic. This workshop aims to explore the contextual factors that contribute to reduced uptake among older adults in order to understand how to design digital technologies that will be appealing to and work for them, fitting with recent calls for more holistic approaches to designing for older adults. Going beyond standard accessibility considerations, and aiming to inform design of technologies for the general population rather than the design of senior-friendly variants of such tools, we will generate a set of principles for developing tools that older adults can and will use

The harm in conflating aging with accessibility

Including older adults as full stakeholders in digital society. </p

E-cadherin expression and bromodeoxyuridine incorporation during development of ovarian inclusion cysts in age-matched breeder and incessantly ovulated CD-1 mice

BACKGROUND: Female CD-1/Swiss Webster mice subjected to incessant ovulation for 8 months and 12-month breeder mice both developed ovarian inclusion cysts similar to serous cystadenomas. The majority of cysts appeared to be dilated rete ovarii tubules, but high ovulation number resulted in more cortical inclusion cysts. We hypothesized that comparison of inclusion cyst pathology in animals of the same age, but with differences in total lifetime ovulation number, might allow us to determine distinguishing characteristics of the two types of cyst. METHODS: Ovaries from breeder mice (BR) or females subjected to incessant ovulation (IO) were compared at 6-, 9- and 12-months of age. Ovaries were serially sectioned and cysts characterized with regard to location and histology, E-cadherin immunoreactivity and rates of BrdU incorporation. RESULTS: Inclusion cysts developed with age in BR and IO ovaries. The majority of cysts were connected to the ovarian hilus. Two cortical inclusion cysts were observed in ten IO ovaries and one in ten BR ovaries. Low or no E-cadherin immuno-staining was seen in the OSE of all mice studied. Conversely, strong membrane immuno-staining was observed in rete ovarii epithelial cells. Variable E-cadherin immunoreactivity was seen in cells of hilar inclusion cysts, with strong staining observed in cuboidal ciliated cells and little or no staining in flat epithelial cells. Two of the three cortical cysts contained papillae, which showed E-cadherin immuno-staining at the edge of cells. However hilar and cortical cysts were not distinguishable by morphology, cell type or E-cadherin immunoreactivity. BrdU incorporation in cyst cells (1.4% [95% CI: 1.0 to 2.1]) was greater than in OSE (0.7% [95% CI: 0.4 to 1.2]) and very few BrdU-labeled cells were observed in rete ovarii at any age. Incessant ovulation significantly increased BrdU incorporation in OSE of older animals. CONCLUSION: These experiments confirm ovarian inclusion cysts develop with age in the CD-1 mouse strain, irrespective of total ovulation burden. We conclude longer periods of incessant ovulation do not lead to significant changes in inclusion cyst formation or steroidogenesis in CD-1 mice and inclusion cyst type can not be distinguished by morphology, cell proliferation rate or E-cadherin immunoreactivity

Crisis management: how prepared are Australian schools?

Abstract not available