BAC locations on the SB chromosome

Blastn result of the end sequences of six BAC clones in the SB genome (Si_gnG). BAC clones (SW_Ba): 073A18, 073A22, 073E03, 073E17, 073G23, and 145M22. README file summarizes the scaffolds and cytogenetic locations of the BACs in the SB chromosome

Comparison of the pseudo SB and pseudo Sb sequences

The comparison file used to construct supplementary figure 1. The blastn results comparing the pseudo SB and pseudo Sb sequences

Pseudo SB sequence

The SB sequence used to construct supplementary figure 1. Sequences of scaffold06568, scaffold05266, and scaffold01957 from the SB genome (Si_gnG), together with a 1 Mb NNN gap, are concatenated as the pseudo SB sequence. See README file for the scaffold order and orientation

S. geminata contig 000043F sequence

This contig contains the fragment C homolog in the S. geminata draft genome (unpublished)

Sequences of 6 contigs in the Sb supergene

Sequences of the 6 Sb contigs used for the comparison between the SB and Sb supergenes in supplementary figure 1. Contigs were assembled with Pacbio reads using Falcon assembler (v0.5.0) and Quiver for polishing

Fragment C sequence from the SB genome

Sequence is obtained from scaffold01957 of the SB genome (Si_gnG). Translocation of fragment C in the Sb genome suggests two historical overlapping inversions

Blast result of BAC_145M24 in the S. invicta Sb genome

A BLASTN search to query the ~93 kb SB sequence bounded by the two end sequences of BAC_145M24 against a preliminary PacBio assembly of the Sb genom

25 Kb insertion

A slice of 25 Kb DNA sequence from Sinv_littleb_tw_t2p_000058F contig

End sequences for 6 S. invicta BAC clones

End sequencing of the BAC clones with T7P and SP6 primers. Sequence ends were trimmed for high quality using DNA Baser (v4.36.0). BAC clones (SW_Ba) are: 073A18, 073A22, 073E03, 073E17, 073G23, and 145M24

Pseudo Sb sequence

The Sb sequence used to construct supplementary figure 1. Sequences of contigs 000181F-2, 000396F, 000181F-1, 000102F-a, 000151F, and 000058F from the Sb genome (Sinv_littleb_tw_t2p), together with a 1 Mb NNN gap, are concatenated as the pseudo Sb sequence. See README file for the contig order and orientation