AVANÇOS DO USO DE ENZIMAS NA NUTRIÇÃO DE TILÁPIAS

O gênero tilápia engloba mais de 70 espécies originárias do continente africano, são apreciadas pelo mercado consumidor devido ao sabor suave e aroma agradáveis, e mercado produtor por apresentar rápido crescimento e desenvolvimento em curto período de produção, boa aceitabilidade de ração e boa conversão alimentar, quando alimentadas com dietas que atendam as exigências nutricionais da espécie. Mediante importância da espécie para a aquicultura, a nutrição de tilápias vem avançando. As enzimas digestivas podem ser adicionadas nas formulações de rações para peixes com a finalizada de auxiliar aquelas de produção endógena ou para favorecer a absorção de nutrientes que estavam indisponíveis pela ausência de produção endógena. Com a presente revisão, objetivou-se apresentar os avanços obtidos com a suplementação enzimática na tilapicultura. As enzimas são indis­pensáveis para a realização dos processos metabólicos nos seres vivos, pois catalisam as reações bioquímicas que acontecem no organismo.  A suplementação com enzimas digestivas exógenas em dietas para tilápias melhora os coeficientes de digestibilidade dos nutrientes, reduz fatores antinutricionis dos alimentos, promove ganhos em crescimento e desempenho corporais e pode promover o aumento da superfície de absorção dos enterócitos, no entanto, para composição centesimal da carcaça os resultados encontrados são escassos e inconclusivos, variando de acordo com a enzima suplementada

Characterization of experimental dental research using animals

Objective: To determine the profile of experimental dental research using animals. Methods: The research comprised all the 4141 abstracts existent in the books of annals from the 22nd and 23rd Annual meetings of the Brazilian Society of Dentistry Research and the sample was composed of 377 studies (9.1%). The variables analyzed were: area of knowledge, type of institution, State of the country, type of animal and body part used, occurrence of animal sacrifice, mention of the Research Ethics Committee, receipt of funding and type of financing agency. Results: The largest number of studies concentrated on the areas of Buccomaxillofacial Surgery (27.3%) and Basic Sciences (21.2%). The Public Universities were responsible for 74% of the researches, and the State Institutions were outstanding (82.4%). The State of São Paulo was responsible for 74.1% of the studies. Rats (67.1%) and rabbits (11.1%) were the most frequently used animals, and 68.2% of the animals were sacrificed. The oral cavity was used in 50.1% of the researches and the mandible in 59%. Only 1.9% of the studies mentioned the Research Ethics Committee and 26.3% reported that they received funding. Conclusion: In Dentistry, studies involving animals are predominant in the areas of buccomaxillofacial surgery and basic sciences, with rats andrabbits being most frequently used. A significant number of guinea pigs are sacrificed during or at the end of the experiments

Assessment of a DNA vaccine encoding an anchored-glycosylphosphatidylinositol tegumental antigen complexed to protamine sulphate on immunoprotection against murine schistosomiasis

Protamine sulphate/DNA complexes have been shown to protect DNA from DNase digestion in a lipid system for gene transfer. A DNA-based vaccine complexed to protamine sulphate was used to induce an immune response against Schistosoma mansoni anchored-glycosylphosphatidylinositol tegumental antigen in BALB/c mice. The protection elicited ranged from 33 to 44%. The spectrum of the elicited immune response induced by the vaccine formulation without protamine was characterized by a high level of IgG (IgG1 > IgG2a). Protamine sulphate added to the DNA vaccine formulation retained the green fluorescent protein encoding-plasmid longer in muscle and spleen. The experiments in vivo showed that under protamine sulphate effect, the scope of protection remained unchanged, but a modulation in antibody production (IgG1 = IgG2a) was observed

Transfusion reaction and hemovigilance: An imperative discussion in Brazilian hemotherapy services

Transfusion of blood components is considered safer, but it took years to reach this level. One of the most effective ways to make blood transfusion a safer practice is hemovigilance, which provides important data, including the history of feared transfusion reactions. In recent years in Brazil, there has been an improvement in the reporting of transfusion reactions, however due to the great diversity of hematology services, there are still transfusion reactions. The aims of this study were described the main types of transfusion reactions, as well as to evaluate the underreporting importance of transfusion occurrences of hemotherapy services in Brazil

Adoptive Transfer of Bone Marrow-Derived Monocytes Ameliorates Schistosoma mansoni -Induced Liver Fibrosis in Mice

Submitted by Sandra Infurna ([email protected]) on 2019-08-27T16:02:21Z No. of bitstreams: 1 LigiaPaiva_PatriciaBozza_etal_IOC_2019.pdf: 2005612 bytes, checksum: 2e2972526ba6a74ba6c014cbe3ab3614 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2019-08-27T16:12:39Z (GMT) No. of bitstreams: 1 LigiaPaiva_PatriciaBozza_etal_IOC_2019.pdf: 2005612 bytes, checksum: 2e2972526ba6a74ba6c014cbe3ab3614 (MD5)Made available in DSpace on 2019-08-27T16:12:39Z (GMT). No. of bitstreams: 1 LigiaPaiva_PatriciaBozza_etal_IOC_2019.pdf: 2005612 bytes, checksum: 2e2972526ba6a74ba6c014cbe3ab3614 (MD5) Previous issue date: 2019Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Universidade Federal de Pernambuco. Centro Acadêmico de Vitória. Vitória de Santo Antão, PE, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Liver diseases are a major health problem worldwide leading to high mortality rates and causing a considerable economic burden in many countries. Cellular therapies as potential treatments for liver diseases have proven beneficial in most of the conditions. In recent years, studies involving therapy with bone marrow cells have been implemented to promote liver regeneration and to reduce hepatic fibrosis, however identifying the cell population present in the bone marrow that is responsible for hepatic improvement after therapy is still necessary. The aim of the present study was the evaluation of the therapeutic efficacy of monocytes obtained from bone marrow in fibrosis resulting from S. mansoni infection in C57BL/6 mice. Monocytes were isolated by immunomagnetic separation and administered to the infected animals. The effects of treatment were evaluated through morphometric, biochemical, immunological and molecular analyzes. Monocyte therapy promoted reduction of liver fibrosis induced by S. mansoni infection, associated with a decrease in production of inflammatory and pro-fibrogenic mediators. In addition, monocyte infusion caused downregulation of factors associated with the M1 activation profile, as well as upregulation of M2reg markers. The findings altogether reinforce the hypothesis that the predominance of M2reg macrophages, producers of immunosuppressive cytokines, may favor the improvement of hepatic fibrosis in a preclinical model, through fibrous tissue remodeling, modulation of the inflammatory response and fibrogenesis