24 research outputs found
Production and Isolation of Azaspiracid-1 and -2 from Azadinium spinosum Culture in Pilot Scale Photobioreactors
Azaspiracid (AZA) poisoning has been reported following consumption of contaminated shellfish, and is of human health concern. Hence, it is important to have sustainable amounts of the causative toxins available for toxicological studies and for instrument calibration in monitoring programs, without having to rely on natural toxin events. Continuous pilot scale culturing was carried out to evaluate the feasibility of AZA production using Azadinium spinosum cultures. Algae were harvested using tangential flow filtration or continuous centrifugation. AZAs were extracted using solid phase extraction (SPE) procedures, and subsequently purified. When coupling two stirred photobioreactors in series, cell concentrations reached 190,000 and 210,000 cell·mL−1 at steady state in bioreactors 1 and 2, respectively. The AZA cell quota decreased as the dilution rate increased from 0.15 to 0.3 day−1, with optimum toxin production at 0.25 day−1. After optimization, SPE procedures allowed for the recovery of 79 ± 9% of AZAs. The preparative isolation procedure previously developed for shellfish was optimized for algal extracts, such that only four steps were necessary to obtain purified AZA1 and -2. A purification efficiency of more than 70% was achieved, and isolation from 1200 L of culture yielded 9.3 mg of AZA1 and 2.2 mg of AZA2 of >95% purity. This work demonstrated the feasibility of sustainably producing AZA1 and -2 from A. spinosum cultures
Changes over time in the weight of tablets stored at 25°C/60% RH in comparison with T0.
<p>The vertical bars represent the 95% confidence interval limits for the measured weight variation.</p
Changes over time in the hardness (A) and friability (B) of tablets stored at 25°C/60% RH in comparison with T0.
<p>The vertical bars represent the 95% confidence interval limits for the measured hardness (A) and friability (B) variation.</p
Changes in misoprostol tablet characteristics and content in misoprostol and misoprostol degradation products after 24 and 48 h exposition to usual European humidity and room temperature.
<p>CI: confidence interval; NA: not available; *: p<0.05 vs T0.</p><p>Changes in misoprostol tablet characteristics and content in misoprostol and misoprostol degradation products after 24 and 48 h exposition to usual European humidity and room temperature.</p
Change over time in medical abortion as percentage of first trimester abortions.
<p>Note below the figure: references regarding figures may be found at the following addresses. Scotland: <a href="http://www.isdscotlandarchive.scot.nhs.uk/isd/6207.html" target="_blank">http://www.isdscotlandarchive.scot.nhs.uk/isd/6207.html</a>; Sweden: <a href="http://www.socialstyrelsen.se/statistik/statistikefteramne/aborter" target="_blank">http://www.socialstyrelsen.se/statistik/statistikefteramne/aborter</a>; France: <a href="http://www.drees.sante.gouv.fr/les-interruptions-volontaires-de-grossesse-en-2011,11149.html" target="_blank">http://www.drees.sante.gouv.fr/les-interruptions-volontaires-de-grossesse-en-2011,11149.html</a>; Switzerland: <a href="http://www.bfs.admin.ch/bfs/portal/de/index/themen/14/02/03/key/03.html" target="_blank">http://www.bfs.admin.ch/bfs/portal/de/index/themen/14/02/03/key/03.html</a>; Finland: <a href="http://www.stakes.fi/" target="_blank">www.stakes.fi/</a>; UK: <a href="https://www.gov.uk/government/collections/abortion-statistics-for-england-and-wales" target="_blank">https://www.gov.uk/government/collections/abortion-statistics-for-england-and-wales</a>; Germany: <a href="https://www.destatis.de/DE/ZahlenFakten/GesellschaftStaat/Gesundheit/Schwangerschaftsabbrueche/Schwangerschaftsabbrueche.html" target="_blank">https://www.destatis.de/DE/ZahlenFakten/GesellschaftStaat/Gesundheit/Schwangerschaftsabbrueche/Schwangerschaftsabbrueche.html</a>; Norway: <a href="http://www.ssb.no/en/abort" target="_blank">http://www.ssb.no/en/abort</a>; Denmark: <a href="http://www.dst.dk/da/" target="_blank">http://www.dst.dk/da/</a>; Portugal: <a href="http://www.spdc.pt/files/Relatorio_Interrupo_de_gravidez_2012.pdf" target="_blank">http://www.spdc.pt/files/Relatorio_Interrupo_de_gravidez_2012.pdf</a>.</p
Changes over time in the misoprostol concentration of tablets stored at 25°C/60% RH in comparison with T0.
<p>The vertical bars represent the 95% confidence interval limits for the measured content variation.</p
Production and preparative isolation of azaspiracid-1 and -2 from Azadinium spinosum culture in stirred photobioreactors
Long Reads for Long-Term Goals : A Genome Resource for Lavander Genetic Improvement
International audienceLavender (Lavandula angustifolia L.) is both a patrimonial and economically important species, used for its essential oils in cosmetics and perfumery products. Naturally adapted to poor soil and dry environment, lavender represents a promising culture in the context of climate change. However, increasing pathogenic pressure related to climate change challenges present varieties yields and quality of essential oils. The cultivar 'Maillette', that is most-valued for the quality of its essential oil, is widely used in genetic improvement programs in France. Useful genomic resources available for these programs remain nevertheless scarce, despite a short-reads assembled genome available. To support the emerging programs for genetic improvement in lavender, we generated genomic resources based on long-reads sequences, in complement with Illumina short-reads sequences