The inner radio jet region and the complex environment of SS433

We present multi-frequency VLBA+VLA observations of SS433 at 1.6, 5 and 15 GHz. These observations provide the highest angular resolution radio spectral index maps ever made for this object. Motion of the components of SS433 during the observation is detected. In addition to the usual VLBI jet structure, we detect two radio components in the system at an anomalous position angle. These newly discovered radio emitting regions might be related to a wind-like equatorial outflow or to an extension of the accretion disk. We show that the radio core component is bifurcated with a clear gap between the eastern and western wings of emission. Modelfitting of the precessing jets and the moving knots of SS433 shows that the kinematic centre -- i.e. the binary -- is in the gap between the western and eastern radio core components. Spectral properties and observed core position shifts suggest that we see a combined effect of synchrotron self-absorption and external free-free absorption in the innermost AU-scale region of the source. The spatial distribution of the ionized matter is probably not spherically symmetric around the binary, but could be disk-like.Comment: Accepted for publication by Astronomy and Astrophysic

Molecular footprint of drug-selective pressure in a human immunodeficiency virus transmission chain

Known human immunodeficiency virus (HIV) transmission histories are invaluable models for investigating the evolutionary and transmission dynamics of the virus and to assess the accuracy of phylogenetic reconstructions. Here we have characterized an HIV-1 transmission chain consisting of nine infected patients, almost all of whom were treated with antiviral drugs at later stages of infection. Partial pol and env gp41 regions of the HIV genome were directly sequenced from plasma viral RNA for at least one sample from each patient. Phylogenetic analyses in pol using likelihood methods inferred an evolutionary history not fully compatible with the known transmission history. This could be attributed to parallel evolution of drug resistance mutations resulting in the incorrect clustering of multidrug-resistant virus. On the other hand, a fully compatible phylogenetic tree was reconstructed from the env sequences. We were able to identify and quantify the molecular footprint of drug-selective pressure in pol using maximum likelihood inference under different codon substitution models. An increased fixation rate of mutations in the HIV population of the multidrug-resistant patient was demonstrated using molecular clock modeling. We show that molecular evolutionary analyses, guided by a known transmission history, can reveal the presence of confounding factors like natural selection and caution should be taken when accurate descriptions of HIV evolution are required.status: publishe

Kinematics of parsec-scale structures in AGN: the 2cm VLBA Survey

We are investigating the kinematics of jets in active galactic nuclei on parsec scales by studying a representative population of sources. This study is being carried out using the Very Long Baseline Array at 15 GHz, with more than 800 images taken since 1994. In this contribution we present an overview of the diversity of kinematics for a complete sample of sources.Comment: Proceedings of the 6th European VLBI Network Symposium, Ros E., Porcas R.W., Lobanov, A.P., & Zensus, J.A. (eds), MPIfR, Bonn, Germany. 2 pages, 3 figures, needs evn2002.cls style fil

Variability and Velocity of Superluminal Sources

We investigate the relation between the Doppler factor determined from variations in total flux at 22 and 37 GHz, and the apparent transverse velocity determined from VLBA observations at 2 cm. The data are consistent with the relativistic beaming theory for compact radio sources, in that the distribution of beta_{app}/delta_{var}, for 30 quasars, is roughly consistent with a Monte Carlo simulation. The intrinsic temperature appears to be ~2x10^{10} K, close to the "equipartition value" calculated by Readhead (1994). We deduce the distribution of Lorentz factors for a group of 48 sources; the values range up to about gamma=40.Comment: To be published in "Radio Astronomy at the Fringe", ASP Conf. Ser. Vol. 300, J. A. Zensus, M. H. Cohen, & E. Ros (eds.), 8 pages, 3 figures, needs rafringe.st

Interaction of nucleotide excision repair factors RPA and XPA with DNA containing bulky photoreactive groups imitating damages

Interaction of nucleotide excision repair factors-replication protein A (RPA) and Xeroderma pigmentosum complementing group A protein (XPA)-with DNA structures containing nucleotides with bulky photoreactive groups imitating damaged nucleotides was investigated. Efficiency of photoaffinity modification of two proteins by photoreactive DNAs varied depending on DNA structure and type of photoreactive group. The secondary structure of DNA and, first of all, the presence of extended single-stranded parts plays a key role in recognition by RPA. However, it was shown that RPA efficiently interacts with DNA duplex containing a bulky substituent at the 5′-end of a nick. XPA was shown to prefer the nicked DNA; however, this protein was cross-linked with approximately equal efficiency by single-stranded and double-stranded DNA containing a bulky substituent inside the strand. XPA seems to be sensitive not only to the structure of DNA double helix, but also to a bulky group incorporated into DNA. The mechanism of damage recognition in the process of nucleotide excision repair is discussed.</p

Interaction of nucleotide excision repair factors RPA and XPA with DNA containing bulky photoreactive groups imitating damages

Interaction of nucleotide excision repair factors-replication protein A (RPA) and Xeroderma pigmentosum complementing group A protein (XPA)-with DNA structures containing nucleotides with bulky photoreactive groups imitating damaged nucleotides was investigated. Efficiency of photoaffinity modification of two proteins by photoreactive DNAs varied depending on DNA structure and type of photoreactive group. The secondary structure of DNA and, first of all, the presence of extended single-stranded parts plays a key role in recognition by RPA. However, it was shown that RPA efficiently interacts with DNA duplex containing a bulky substituent at the 5′-end of a nick. XPA was shown to prefer the nicked DNA; however, this protein was cross-linked with approximately equal efficiency by single-stranded and double-stranded DNA containing a bulky substituent inside the strand. XPA seems to be sensitive not only to the structure of DNA double helix, but also to a bulky group incorporated into DNA. The mechanism of damage recognition in the process of nucleotide excision repair is discussed.</p

Multi-band optical micro-variability observations of BL Lacertae

We have observed BL Lacertae in the B, R and I bands for 2 nights in July, 1999, and 3 nights in July, 2001. The observations resulted in almost evenly sampled light curves, with an average sampling interval of ~5 min. The source is significantly variable in all bands. On average, the variability amplitude increases from ~5% in the I band, to ~5.5% in the R and ~6.5% in the B band light curves. The rising and decaying time scales are comparable within each band, but they increase from the B, to R and I band light curves. The optical power spectrum shows a red noise component with a slope of ~ -2. Cross-correlation analysis shows that in most cases the delay between the variations in the B and I band light curves is less than ~ 0.4 hrs. The cross-correlation functions are asymmetric, implying complex delays of the I band variations with respect to the B band variations. Furthermore, in one case we find that the I band variations are significantly delayed (by ~0.2 hrs) with respect to the B band variations. We also detect significant spectral variations: the spectrum becomes steeper as the flux increases, and the flattest spectral index corresponds to the maximum B band flux. Our results imply that the fast, intra-night variations of the source correspond to perturbations of different regions in the jet which cause localized injections of relativistic particles on time scales much sorter that the average sampling interval of the light curves. The variations are controlled by the cooling and light crossing time scales, which are probably comparable.Comment: Accepted for publication in A&

Intrinsic Brightness Temperatures of AGN Jets

We present a new method for studying the intrinsic brightness temperatures of the parsec-scale jet cores of Active Galactic Nuclei (AGN). Our method uses observed superluminal motions and observed brightness temperatures for a large sample of AGN to constrain the characteristic intrinsic brightness temperature of the sample as a whole. To study changes in intrinsic brightness temperature, we assume that the Doppler factors of individual jets are constant in time as justified by their relatively small changes in observed flux density. We find that in their median-low brightness temperature state, the sources in our sample have a narrow range of intrinsic brightness temperatures centered on a characteristic temperature, T_int = 3 x 10^10 K, which is close to the value expected for equipartition, when the energy in the radiating particles equals the energy stored in the magnetic fields. However, in their maximum brightness state, we find that sources in our sample have a characteristic intrinsic brightness temperature greater than 2 x 10^11 K, which is well in excess of the equipartition temperature. In this state, we estimate the energy in radiating particles exceeds the energy in the magnetic field by a factor of ~ 10^5. We suggest that the excess of particle energy when sources are in their maximum brightness state is due to injection or acceleration of particles at the base of the jet. Our results suggest that the common method of estimating jet Doppler factors by using a single measurement of observed brightness temperature and/or the assumption of equipartition may lead to large scatter or systematic errors in the derived values.Comment: 4 pages, 2 figures, Accepted to Appear in ApJ Letter

Doppler Boosting, Superluminal Motion, and the Kinematics of AGN Jets

We discuss results from a decade long program to study the fine-scale structure and the kinematics of relativistic AGN jets with the aim of better understanding the acceleration and collimation of the relativistic plasma forming AGN jets. From the observed distribution of brightness temperature, apparent velocity, flux density, time variability, and apparent luminosity, the intrinsic properties of the jets including Lorentz factor, luminosity, orientation, and brightness temperature are discussed. Special attention is given to the jet in M87, which has been studied over a wide range of wavelengths and which, due to its proximity, is observed with excellent spatial resolution. Most radio jets appear quite linear, but we also observe curved non-linear jets and non-radial motions. Sometimes, different features in a given jet appear to follow the same curved path but there is evidence for ballistic trajectories as well. The data are best fit with a distribution of Lorentz factors extending up to gamma ~30 and intrinsic luminosity up to ~10^26 W/Hz. In general, gamma-ray quasars may have somewhat larger Lorentz factors than non gamma-ray quasars. Initially the observed brightness temperature near the base of the jet extend up to ~5x10^13 K which is well in excess of the inverse Compton limit and corresponds to a large excess of particle energy over magnetic energy. However, more typically, the observed brightness temperatures are ~2x10^11 K, i.e., closer to equipartition.Comment: 10 pages, 12 color figures; proceedings of the 5th Stromlo Symposium: Disks, Winds, and Jets - from Planets to Quasars; accepted in Astrophysics & Space Scienc