IN VITRO ANALYSIS OF ANTICANCER POTENTIAL OF TRIGONELLA FOENUM-GRAECUM

Objective: The recent work was carried out to evaluate the presence of bioactive compounds and anticancer activities of selected plant Trigonella foenum-graecum extract. Methods: The crude extraction of whole plant was carried out using petroleum ether as a solvent by cold percolation as well as hot percolation method (Soxhlet method) both. The presence of phytochemicals was determined using standard protocols. The anticancer activities were evaluated by sulforhodamine B (SRB) dye assay method using Mitomycin-C (anticancer drug) as a positive control. Results: The qualitative analysis of plant extract showed the presence of bioactive compounds, namely, protein, alkaloids, steroids, phenolics, and flavonoids. The plant extract was tested for in vitro anticancer activity against human liver cancer (HEP-2) and colon cancer (HT-29) cell lines using SRB assay. The plant extract showed significant results against HT-29 and HEP-2 cancer cell lines. Conclusion: The study concluded that the extract of T. foenum-graecum can be further carefully used in herbal formulations for cancer therapy

Cytogenetic studies of fish species Horabagrus nigricollaris, Puntius denisonii and Puntius sarana subnasutus endemic to the Western Ghats

Cytogenetic studies of fish species Horabagrus nigricollaris, Puntius denisonii and Puntius sarana subnasutus endemic to the Western Ghat

Evaluation of colonization and mutualistic endophytic symbiosis of Escherichia coli with tomato and Bermuda grass seedlings

Escherichia coli is generally considered a bacterium associated with animal microbiomes. However, we present evidence that E. coli may also mutualistically colonize roots of plant species, even to the extent that it may become endophytic in plants. In this study we used GFP tagged E. coli to observe its colonization and effects on tomato (Solanum lycopersicum) and Bermuda grass (Cynodon dactylon) seedling development and growth. Inoculation with the bacterium significantly improved root development of both seedlings tested. Treatment also increased the photosynthetic pigments in Bermuda grass seedlings. However, effects on shoot length in both seedlings were not significant. This bacterium was found to produce indole acetic acid (IAA) up to 8.68 ± 0.43 µg ml−1 in the broth medium amended with tryptophan. Effects on seedling root growth could, in part, be explained by IAA production. Bacteria successfully colonized the root surfaces and interiors of both seedlings. Tagged bacteria expressing the GFP were observed in the vascular tissues of Bermuda grass seedling roots. Seedlings with bacteria showed greater survival and were healthier than seedlings without bacteria, indicating that E. coli set up a successful mutualistic symbiosis with seedlings. E. coli is not commonly considered to be a plant endophyte but is more generally considered to be a crop contaminant. In this study we show that E. coli may also be an endophyte in plant tissues

Gljivice povezane s pobačajem i jalovosću u koza i ovaca.

Investigations into the association of mycotic agents in the causation of reproductive disorders in 115 does and 163 ewes were conducted. Only one aborted ewe yielded Aspergillus fumigatus whereas from endometritis the overall fungal isolation was 7.5% in does and 7.5% in ewes. The predominance of Aspergillus spp. was to the extent of 57%. In does, fungal agent association was 11%, while in ewes it was 7% irrespective of type of disorder. In endometritic ewes the isolation of Aureobasidium pullulans, Altenaria spp. and Syncephalastrum spp. is noteworthy. Trichoderma spp. and Rhizoctonia spp. were also encountered in this study and which are rarely reported.Istraživana je povezanost gljivičnih uzročnika s poremećajima u razmnožavanju 115 koza i 163 ovce. U pobačenom materijalu jedne ovce utvrđena je gljivica Aspergillus fumigatus, dok su iz upalnog iscjetka od endometritisa gljivice pronađene u 7,5% koza i 7,0% ovaca. Aspergillus spp. je dominirala sa 57%. Gljivice su bile uzrokom poremećaja u razmnožavanju u 11% koza i 7% ovaca. U ovaca s endometritisom nađene su gljivice Auerobasidium pullulans, Altenaria spp. i Syncephalastrum spp. U ovim istraživanjima utvrđeni su i inače rijetki nalazi Trichoderma spp. i Rhizoctonia spp

Gljivice povezane s pobačajem i jalovosću u koza i ovaca.

Investigations into the association of mycotic agents in the causation of reproductive disorders in 115 does and 163 ewes were conducted. Only one aborted ewe yielded Aspergillus fumigatus whereas from endometritis the overall fungal isolation was 7.5% in does and 7.5% in ewes. The predominance of Aspergillus spp. was to the extent of 57%. In does, fungal agent association was 11%, while in ewes it was 7% irrespective of type of disorder. In endometritic ewes the isolation of Aureobasidium pullulans, Altenaria spp. and Syncephalastrum spp. is noteworthy. Trichoderma spp. and Rhizoctonia spp. were also encountered in this study and which are rarely reported.Istraživana je povezanost gljivičnih uzročnika s poremećajima u razmnožavanju 115 koza i 163 ovce. U pobačenom materijalu jedne ovce utvrđena je gljivica Aspergillus fumigatus, dok su iz upalnog iscjetka od endometritisa gljivice pronađene u 7,5% koza i 7,0% ovaca. Aspergillus spp. je dominirala sa 57%. Gljivice su bile uzrokom poremećaja u razmnožavanju u 11% koza i 7% ovaca. U ovaca s endometritisom nađene su gljivice Auerobasidium pullulans, Altenaria spp. i Syncephalastrum spp. U ovim istraživanjima utvrđeni su i inače rijetki nalazi Trichoderma spp. i Rhizoctonia spp

Differential pulse polarographic determination of tetramethylthiuram disulphide

402-405A differential pulse polarographic method for the determination of tetramethylthiuram disulphide (TMTD) by making use of its reaction with copper(I) perchlorate in acetonitrile has been developed. TMTD reacts with copper(I) perchlorate (1:1 molar ratio) and exhibits a well-defined, diffusion-controlled peak at - 120 mV showing five times more sensitivity than the one obtained due to TMTD alone. The linear relationship between concentration of TMTD and current intensity of peak at - 120 m V can be successfully adapted to the analysis of some fungicide and rubber accelerator formulations based on TMTD

Not Available

Not AvailableGenotoxicity was assessed in Channa punctatus, collected during different seasons by using micronuclei test. The results showed that there was seasonal variation in frequency of micronuclei in C. punctatus. It was 0.15′ 0.06%(maximum) during spring season and nil in monsoon season.Not Availabl

Analysis of Government website for web-registration to assess pattern of the Covid pandemic

Introduction: Covid-19 is an unprecedented challenge in our times leaving a trail of destruction and mayhem affecting almost all of us during the last 2 years. Various data sources are available around the globe to measure its impact using various yardsticks. Material and Methods: By carefully looking at data available at the website maintained by Government of India, we can draw some useful conclusions. Results: There is a dip in the number of online registrations at our hospital coinciding with second wave and resultant lockdown. Conclusion: Tracing digital footprints of an event as huge as the Covid pandemic may help us for future planning when we learn its lessons well