Antibiotic resistance of microbial community of the lake Baikal ecosystem in the area of Listvyanka, Slyudyanka and Baikalsk

The article presents the results of study of antibiotic resistance of bacteria isolated from the lake Baikal ecosystem. It was revealed that in the littoral zone of the lake Baikal (area of Listvyanka, Baikalsk, Slyudyanka) under the anthropogenic impact there are strains of microorganisms resistant to a wide range of antibiotics. Strains resistant to all antibiotics are 1 % of the isolated bacterial strains; strains sensitive to all antibiotics are 6 %; strains isolated from the drill samples of bottom sediments are 80 %. This phenomenon can be considered as an indicator of anthropogenic influence

PECULIARITIES OF FORMATION OF EVOKED POTENTIALS IN PATIENTS WITH LUMBAR SPINE STENOSIS

At the analysis of neuromyographic indices of patients with central spinal stenosis it was determined that evident decrease of electroneuromyographic parameters corresponds to the clinical manifestations of stenosis, that give an indication offunctional suppression of motoneurons in consequence of metastasis ad nervos. Obtained data allows the clinicians to develop an adequate and pathogenetically proved scheme of treatment of patients with spinal stenosis

Functional diagnostics and acupuncture in patients with cervical osteochondrosis

We developed an algorithm for spinal diagnostics to optimize the diagnosis and treatment of patients with cervical osteochondrosis. This algorithm includes physical and neurological examination of a patient, radiological methods, multi-layer spiral CT (MRI) of cervical spine, densitometry and electroneuromyography (ENMG) and is used in practical work of neurosurgical unit of Irkutsk Scientific Center of Surgery and Traumatology. We managed 40 patients with cervical osteochondrosis in period I-III localized in CIII-CIV, CV-CV CV-CW Cn-CVIl segments and with severe muscular tonic syndrome (cervicalgia, cervical cranialgia, cervicobrachialgia). Patients with osteochondrosis in period I-II had medium decreased conduction of peripheral nerve motor fibers on both sides of spine, which indicates the lesion of some motor fibers. In patients with osteochondrosis in period III in case of protrusion and herniation of intervertebral discs the ENMG values corresponded to the increase in neurility and conduction velocity of motor nerve and muscle fibers in diseased segment. Conservative treatment was conducted up to the Standard approved by the Ministry of Health of the Russian Federation and was supplemented with acupuncture (9-10 sessions) based on the changes in ENMG values. Performed treatment caused significant decrease in pain syndrome, reduction of neurological manifestations and, as a consequence, positive dynamics of ENMG values. Proposed algorithmfor management of patients with osteochondrosis allowed to diagnose the localization of diseased functional spinal unit, to reveal problem peripheral nerves and, as a result, to choose adequate treatment tactics and to estimate its effectiveness

CERVICOBRACHIAL SYNDROME IN PATIENTS WITH SPINAL OSTEOCHONDROSIS

The article presents clinical profile of patients with cervical osteochondrosis and cervicobrachial syndrome, results of cliniconeurological examination including X-ray diagnostics (plain frontal and lateral radiography of cervical spine, functional tests, frontal and lateral X-ray imaging of large joints of upper limbs), MRI of cervical spine, stimulation electroneuromyography and osteodensimetry. Statistical processing with definition of nonparametric test and correlation coefficient was performed using SPSS 22.0.0 software. Pearson and Spearman nonparametric tests were used for correlation analysis. Examination of patients with cervicobrachial syndrome revealed that bone tissue condition of a limb with pain syndrome slightly differs from the bone tissue condition of a healthy limb and is within normal range. Neuromyography showed that abnormality of a functional condition of studied nerves of upper limbs was not pronounced and was registered on both limbs. As a result of our research we can suppose that asymmetric abnormal focus inhibits peripheral and central chains of locomotor system both on injured and healthy limbs. Differences in functional condition of peripheral nerves of upper limbs in patients with cervicobrachial syndrome and healthy people are statistically significant and allow us to consider them as a sign of decompensation which prevent healthy performance

GENERAL CHARACTERISTICS OF PATIENTS WITH OSTEOCHONDROSIS OF THE CERVICAL SPINE WITH NECK AND UPPER EXTREMITY PAIN

1n 2015-2016 in Scientific-Clinical Department of Neurosurgery of 1rkutsk Scientific Center of Surgery and Traumatology, 22 patients (11 men and 11 women) were examined for osteochondrosis of the cervical spine and neck and upper extremity pain syndrome. Patients underwent clinical and neurological examination, radial methods of investigation (cervical survey radiography in the direct and lateral projections), functional tests (flexion and extension) and functional tests with weights, MSCT and MR1 of the cervical spine, radiographs of large joints of the upper extremities in two projections, stimulation electroneuromyography and osteodensitometry. The article presents the general clinical characteristics of the data of 22 patients with degenerative-dystrophic pathology of cervical spine and shoulder joint syndrome, and a moderate and severe neck and upper extremity pain syndrome. 1n the evaluation of clinical and neurological symptoms, unilateral cervicobrahialgia syndrome was detected in 19 (86 %) patients, cervicobrachialgia syndrome was noted on both sides in 3 patients, the syndrome of cervicocranialgia was noted in 4 patients. All radiographs showed deforming osteoarthritis of the clavicular-acromial joint (11 persons), shoulder (10 persons), elbow joint (4 persons). MR1 revealed a multilevel pathology of intervertebral discs. According to ENMG, there was a disturbance of the functional state of the peripheral nerves of the upper limbs, which manifested itself on both sides. The state of bone tissue of the limb with the pain syndrome was not significantly different from that of the healthy limb, and generally corresponded to the norm. Thus, the materials presented in the article represent the initial data of the examination of patients with the neck and upper extremity pain syndrome in the department of neurosurgery according to the algorithm developed in 1rkutsk Scientific Center of Surgery and Traumatology

Дифференциальная экспресс-диагностика и экстренная специфическая профилактика трансмиссивных клещевых инфекций в городе Иркутске

The results of differential express diagnostic of tick-borne encephalitis and Lyme borreliosis agents in ticks, removed from pa- tients are present in this article (1995—2004). The entire complex of serolological and genetical methods were used. The specific prophylaxis based on the results of diagnostic had shown to be very effective for both infections. The first data about human ehrli- chiosis and anaplasmosis in Irkutsk region had been obtained. The improvement of diagnostic and prophylactic procedures are pro- posed.В статье представлены результаты дифференциальной экспресс-диагностики возбудителей клещевого энцефалита и иксодовых клещевых боррелиозов в снятых с пострадавших лиц клещах или крови пациентов за период с 1995—2004 гг. Для дифференциальной диагностики применяли комплекс методов, включая стандартные серологические и современные молекулярно-генетические тесты. В зависимости от результатов исследования проводили экстренную специфическую профилактику, которая показала хорошие результаты предупреждения обеих инфекций. Получены первые сведения о воз- можной циркуляции возбудителей моноцитарного эрлихиоза человека и гранулоцитарного анаплазмоза человека на терри- тории Иркутской области. Предложены способы усовершенствования диагностики и профилактики

Findings to the flora of Russia and adjacent countries: New national and regional vascular plant records, 4

With this paper we continue a new annual series, the main purpose of which is to make significant floristic findings from Russia and neighboring countries more visible in Russia and abroad. In total, this paper presents new records for 48 vascular plant species from 6 Eurasian countries, obtained during field explorations, as well as during taxonomic revisions of herbarium materials. For the first time, a new locality of Leontopodium leiolepis is recorded for Russia, Rheum uzengukuushi for China, Rorippa prolifera for Lithuania, Lappula marginata for Kyrgyzstan and Tajikistan, Anthriscus caucalis, Chenopodium ficifolium, Euphorbia prostrata for Uzbekistan, Adonis × hybrida, Potamogeton × franconicus, Solidago × niederederi for the Asian part of Russia, Echinochloa esculenta, Poa jamalinensis, Puccinellia poecilantha for Siberia, Potentilla intermedia for the Caucasus, Rhynchospora alba for the Russian part of Altai, Poa sphondylodes, Veronica beccabunga for Eastern Siberia, Asclepias syriaca for the Republic of Altai, Chimaphila umbellata, Orobanche korshinskyi, Veronica scutellata for the Republic of Buryatia, Cirsium alatum, Thalictrum simplex for the Republic of Crimea, Thymus rariflorus, Th. terekensis for the Republic of Ingushetia, Berberis thunbergii, Crataegus maximowiczii, Prunus serotina for the Republic of Mordovia, Oenothera villosa for the Republic of Tatarstan, Astragalus sulcatus, Galium mollugo for the Republic of Tyva, Phragmites altissimus for the Chelyabinsk Region, Senecio dubitabilis for the Magadan Region, Asclepias syriaca, Galatella villosa, Potentilla recta for the Novosibirsk Region, Dodartia orientalis for the Omsk Region, Viola hultenii for the Sakhalin Region, Phragmites tzvelevii for the Samara Region and the Middle Volga, Jacobaea ferganensis for the Samara Region, Carex media, Impatiens parviflora for the Tyumen Region. There are some more findings which are not new for the region but they contribute significantly to the understanding of species distribution

RESULTS OF GENOTYPING OF STRAINS AND RNA ISOLATES OF TICK-BORNE ENCEPHALITIS VIRUS ISOLATED FROM SICK PEOPLE IN IRKUTSK REGION AND BURYAT REPUBLIC

Genotyping of nine strains and nine isolates of tick-borne encephalitis virus RNA (TBE) isolated from patients in the Irkutsk Region and. the Buryat Republic in 1963-2009 years conducted. The investigations revealed that three TBE virus genotypes are involved in the etiology of the TBE: Ural-Siberian (genotype 3), Far Eastern (genotype 1) and. European (genotype 2). It is found that the TBE virus of Far East and. Ural-Siberian genotypes can cause disease with a wide spectrum of clinical manifestations: acute TBE (focal and. nonlocal forms), chronic TBE (encephalitic form, progressive course.) Prospects of application of molecular genetic techniques for the rapid indication and. identification of the TBE virus in the blood of sick people are shown

BRIEF CHARACTERISTIC OF EUROPEAN GENOTYPE TICK-BORNE ENCEPHALITIS VIRUS STRAINS IDENTIFIED IN SIBERIAN REGION

The molecular-genetic analysis of 13 strains of Western genotype TBEV isolated in Western and Eastern Siberia demonstrated two groups of strains differed geneticallyfrom each other and had a high level of E gene sequences homology within each group. Nevertheless, the heterogeneity of biological propertiesfor some strains within a group was observed

Characteristics of genetic and phenotypic properties of tick-borne encephalitis virus strains isolated from various source on the territory of Eastern Siberia

Since ixodid ticks and their feeders (vertebrates) form the basis of the tick-borne encephalitis virus (TBEV) population, it was interesting to obtain a genetic characteristic of strains isolated from various sources in the territory of Eastern Siberia. In our study, it was found that the circulation of TBEV of genotypes 1, 2, 3, 5 and "polytypic" strains in the territory of Eastern Siberia is maintained both by the main vectors (I. persulcatus ticks) and by their feeders (small and large mammals, birds). The distribution of genotypes in strains groups from ixodid ticks and vertebrates varied. TBEV strains of genotypes 1 and 2 were significantly more often isolated from rodents, and genotype 3 from ixodid ticks (p < 0.05). Isolates of genotype 3 (85.5 %) prevailed in the group of strains isolated from ixodid ticks, of which 29.6 % belonged to "Vasilchenko", and 25.6 % to "Zausaev"subgenotypes. Genotypes 1 and 2 were represented by single isolates (3.3 % and 1.3 %, respectively). Among strains isolated from warm-blooded hosts, the proportion of genotype 1 was 35.3 %, genotype 2 -11.8 %, genotype 3 - 35.3 %. More than half of genotype 3 strains (58.3 %) were related to "Vasilchenko" subgenotype, and the strains of "Zausaev" subgenotype were not detected. The obtained data suggest that ixodid ticks and warm-blooded animals are amplifiers of TBEV of genotypes 3 and 1, respectively. The certain differences in the phenotypic characteristics of strains isolated from ticks and warm-blooded animals have been revealed. It has been found that strains isolated from ticks are more homogeneous in their antigenic characteristics, less heterogeneous in S-feature than strains isolated from warm-blooded sources. The strains isolated from warm-blooded animals demonstrate somewhat greater resistance to warming but reducing the reproduction of the virus at 42 °C, and have greater virulence compared to strains isolated from ticks