LAPACK-Based Software for Subspace Identification

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Isolation and characterization ofmicrosatellitemarkers for Acacia senegal (L.)Willd., amultipurpose arid and semi-arid tree

International audienceAcacia senegal is a multipurpose African tree that improves the soil fertility of degraded areas. The species is exploited mainly for gum arabic, but it also supplies fuel wood and fod- der for animals. Despite its wide distribution in Africa, no microsatellite markers have yet been characterized for this species. In this study, we characterized 11 polymorphic microsatel- lite loci specifically designed for A. senegal and analysed 247 individuals from three popula- tions from Niger. On average, 10.9 alleles per locus were detected and expected heterozygosity ranged from 0.160 to 0.794, showing the ability of the markers to detect genetic diversity in this species

Knelpunten in aquacultuur: enkele risicovolle aspecten van de vroege levensstadia van de vis = Bottlenecks in aquaculture: some risky aspects of early life stages of fish

Aquaculture is a fast growing sector. However, during larviculture and fish husbandry, many bottlenecks still occur. Many problems concern nutritional factors and can be avoided by a clear understanding of the development of the gastrointestinal system of the various fish species. Parameters, such as enterocyte height, the presence of enterocytic supranuclear vacuoles and ‘the point of no return’, can be used to monitor and prevent food deprivation. This information can also be used to compose a feeding regime with rotifers, Artemia and copepods

What is the genetic origin of teak (Tectona grandis L.) introduced in Africa and in Indonesia?

International audienceThe first introduction of Tectona grandis in Indonesia took place between the fourteenth and sixteenth centuries and in Africa in the nineteenth century. A total of 1.1 and 0.3 million ha, respectively were planted in the two areas. The extension of teak plantations often started from these first introductions. Unfortunately, the documentation concerning dates, planting stocks and the sources of origin of the teak imported into the different countries was very inaccurate. In this study, the use of 15 microsatellite molecular markers enabled us to compare 22 exotic teak provenances with 17 provenances of the natural range. Results of the analysis showed that the provenances from South India were not related to the provenances that were first introduced in either Africa or Indonesia. Nearly 95% of teak landraces in Benin, Cameroon, Côte d'Ivoire, Tanza- nia, Togo and Senegal came from North India, and 96% of Indonesian and Ghanaian teak appeared to be very closely linked to Central Laos. The genetic origin of introduced teak was confirmed by the main traits of interest of provenances observed in international trials. Thus, trees from North India had very bad stem forms compared to Laotian and Thai provenances, which generally had good stem forms but low vigour. This genetic knowledge is essential for programmes to develop varieties and to improve the quality of plantations, particularly in Afric

Structure of the specific combining ability between two species of Eucalyptus. II. A clustering approach and a multiplicative model

International audienceThe Eucalyptus breeding program of URPPI (a partnership between CIRAD-Fore t, Centre National de la Recherche Forestie re du Congo, and Unite dÕAforestation Industrielle du Congo) consists of a re- ciprocal recurrent selection scheme developed in the Congo between the two species Eucalyptus urophylla and Eucalyptus grandis. Two approaches are proposed in order to model and predict the speciÞc combining ability (SCA) between these species. The clustering ap- proach uses a simultaneous clustering procedure of the two species based on SCA and reveals heterotic groups coherent with the geographical origins of E. urophylla genotypes. The second approach uses a multiplicative model to partition the SCA into three multiplicative terms explaining 95% of the interactio

Structure of the specific combining ability between two species of Eucalyptus. I. RAPD data

International audienceWithin the context of the reciprocal recurrent selection scheme developed in 1989 by CIRAD-Fore t on Eucalyptus, RAPD essays were performed to assess the genetic diversity in the two species E. urophylla and E. grandis. The molecular markers were split into two parts: the speciÞc markers (present with di¤erent fre- quencies in the two species) and the common markers (present with similar frequencies in the two species). The study analyses the structure of genetic diversity within and between the two species of Eucalyptus. Dif- ferent genetic distances are worked out for use in pre- diction equations of the individual tree trunk volume of hybrids at 38 months. Each distance is expressed as the sum of the general genetic distance and the speciÞc genetic distance. The general genetic distance based on the double presence plus the double absence of bands seems to be an interesting co-variate to use in a factor regression model. Through this model the distance calculated between species explains the general com- bining ability (GCA) and the speciÞc combining ability (SCA) of the phenotypic character with a global coe¦c- ient of determination of 81.6

Gene flow estimation with microsatellites in a Malagasy seed orchard of Eucalyptus grandis

International audienceEucalyptus grandis has a mixed-mating repro- ductive system. Malagasy Eucalyptus seed orchards were established 15 years ago with two aims both based on panmixia: open-pollinated seed production and genetic improvement. The panmixia hypothesis has never been confirmed in the seed orchard. From a seedling seed- orchard stand comprising 349 trees and using data obtained with six selected microsatellite markers, pater- nity analysis was performed for 724 offspring collected on 30 adult trees. Paternity assignment, based on exclu- sion procedures and likelihood-ratio method, was achieved with high accuracy; the exclusion probability value was 0.997. The outcrossing rate was very high (96.7%). More than 50% of potential male trees (199 out of 349) in the seed orchard contributed to pollination for 440 offspring of 30 progenies (8.6% of the basic population). The pollination rate from outside the seed orchard was high (39.2%), but might be due to the small size of this seed orchard. This study showed that "panmixia-like pollination" can be assume

Fast & Furious focal-plane wavefront sensing

We present two complementary algorithms suitable for using focal-plane measurements to control a wavefront corrector with an extremely high-spatial resolution. The algorithms use linear approximations to iteratively minimize the aberrations seen by the focal-plane camera. The first algorithm, Fast & Furious (FF), uses a weak-aberration assumption and pupil symmetries to achieve fast wavefront reconstruction. The second algorithm, an extension to FF, can deal with an arbitrary pupil shape; it uses a Gerchberg–Saxton (GS)-style error reduction to determine the pupil amplitudes. Simulations and experimental results are shown for a spatial-light modulator controlling the wavefront with a resolution of 170×170  pixels. The algorithms increase the Strehl ratio from ∼0.75 to 0.98–0.99, and the intensity of the scattered light is reduced throughout the whole recorded image of 320×320  pixels. The remaining wavefront rms error is estimated to be ∼0.15  rad with FF and ∼0.10  rad with FF-GS

Laboratory study of the impact of repetitive electrical and mechanical stimulation on brown shrimp Crangon crangon

Pulse trawling is currently the best available alternative to beam trawling in the brown shrimp Crangon crangon and Sole Solea solea (also known as Solea vulgaris) fisheries. To evaluate the effect of repetitive exposure to electrical fields, brown shrimp were exposed to the commercial electrodes and pulse settings used to catch brown shrimp (shrimp startle pulse) or Sole (Sole cramp pulse) 20 times in 4 d and monitored for up to 14 d after the first exposure. Survival, egg loss, molting, and the degree of intranuclear bacilliform virus (IBV) infection were evaluated and compared with those in stressed but not electrically exposed (procedural control) and nonstressed, nonexposed (control) brown shrimp as well as brown shrimp exposed to mechanical stimuli. The lowest survival at 14 d (57.3%) occurred in the Sole cramp pulse treatment, and this was significantly lower than in the group with the highest survival, the procedural control (70.3%). No effect of electrical stimulation on the severity of IBV infection was found. The lowest percentage of molts occurred in the repetitive mechanical stimulation treatment (14.0%), and this was significantly lower than in the group with the highest percentage of molts, the procedural control (21.7%). Additionally, the mechanically stimulated brown shrimp that died during the experiment had a significantly larger size than the surviving individuals. Finally, no effect of the shrimp startle pulse was found. Therefore, it can be concluded that repetitive exposure to a cramp stimulus and mechanical stimulation may have negative effects on the growth and/or survival of brown shrimp. However, there is no evidence that electrical stimulation during electrotrawls would have a larger negative impact on brown shrimp stocks than mechanical stimulation during conventional beam trawling