Fungal diversity and community structure on <i>Phragmites australis</i> (poaceae) along a salinity gradient in the scheldt estuary (belgium)

We examined fungal communities on stems and leaf sheaths of common reed (Phragmites australis) in four tidal marshes along a salinity gradient (mesohaline to freshwater) in the Scheldt estuary (Belgium) by direct observation. One hundred and fourteen taxa, the highest number of taxa ever recorded during a survey of phragmiticolous fungi, were found, with 56 taxa (49%) in the ascomycetes, 6 taxa (5%) in the basidiomycetes and 52 taxa (46%) of anamorphic fungi, the latter comprising 30 (26%) coelomycetes and 22 (19%) hyphomycetes. Fungal diversity in tidal marshes compared is lower than that in non-tidal reed marshes. Species distribution on reed litter, investigated by multivariate analysis, was strongly correlated with flooding frequency and salinity. Influences of salinity on the fungal community are discussed and tested by a culture experiment. Taxa were divided into three basic distributional types: taxa present only in the saline-brackish water zone, species present only in the freshwater zone, and a group of eurytopic species found over the entire salinity gradient sampled. Indicator species for each group were determined. In two sites, mesolialine brackish and freshwater, the vertical distribution of mycota was analysed. Higher similarities between the same vertical zones, especially in middle and apical parts of the culms, indicated that terrestrial fungi (no direct contact with tidal exchange water) were less influenced by site-specific characteristics. However, in both sites, a few common indicator species for standing Phragmites culms could be identified. Flooding height (and frequency) is demonstrated to influence vertical species distribution. Generally rather complicated interactions and a narrow niche differentiation among species are revealed for the phragmiticolous fungal community

The crazy-paving pattern: a radiological-pathological correlation

The crazy-paving pattern is a linear pattern superimposed on a background of ground-glass opacity, resembling irregularly shaped paving stones. The crazy-paving pattern is initially described as the pathognomonic sign of alveolar proteinosis. Nowadays this pattern is a common finding on high-resolution CT imaging, and can be seen in a number of acute and chronic diseases. The purpose of this paper is to illustrate different diseases that cause this crazy-paving pattern and to correlate the radiological findings from computed tomography with the histopathological findings

The decomposition of reed leaves in the Scheldt-estuary: spotlight on the Fungi

The poster presents preliminary results on a decomposition-study of reed leaves (Phragmites australis) in one site (PQ 206, near ‘Schor van Doel’). In a brackish reed stand leaves make about 25 % of the above-ground reed biomass, being an important organic pool in the Scheldt-ecosystem and a potential substrate for phragmiticolous fungi. Fungal species composition and fungal biomass (and also some abiotic factors, not presented here) were followed during decomposition in litterbags on the sediment of a reed belt. Fungal biomass was measured using ergosterol quantification. During the survey 40 fungal taxa were found to colonize the leaves from standing green - to standing dead - to litterleaf-stage. Succession and vertical preference of fungal species is demonstrated during growth and decomposition. Leaves, although abscised from the culms, have an important standing dead decomposition with high fungal diversity and biomass (599 µg ergosterol/ g leave, n = 10 corresponding with ! 0.12 g fungus/ g leave) just before abscission. Leaves were almost totally fragmented after a 10 month incubation on the sediment. With fungal crop ranging from 142 µg ergo/ g leave to 346 µg ergo/ g leave showing a steep decline in fungal crop after leaf abscission, a gradual recovery followed by a final decline towards the final stage. Despite the fact that a serious amount of fungal biomass is developed, less species seem to be adapted to colonize the litter leaves

Enhancing Developing Country Access to Eco-Innovation: The Case of Technology Transfer and Climate Change in a Post-2012 Policy Framework

The deployment of eco-innovations in developing countries is a key driver of their contribution to efficiently addressing global environmental challenges. It is also a key driver of markets for eco-innovation and sustainable economic development. This report explores the barriers developing countries face in accessing markets for eco-innovation. It outlines the key considerations policy needs to address to overcome these barriers and discusses the extent to which selected existing policy mechanisms and organisation have achieved this. The key finding of the report is that the majority of existing policy mechanisms fails to recognise the critical importance of developing indigenous eco-innovation capabilities amongst developing country firms. Indigenous eco-innovation capabilities are essential to facilitating both the diffusion of existing ecoinnovations within developing countries and sustainable economic development based on the adoption, adaption and development of environmentally sound technologies that fit with the bespoke conditions faced by developing countries. Building up eco-innovation capabilities in developing countries requires a shift away from the current focus on large project based approaches which emphasise the transfer of the hardware aspects of clean technologies, towards approaches that emphasise flows of codified knowledge (know-how and know-why) and tacit knowledge. Policy also needs to be improved to better respond to the context-specific technological and cultural requirements which vary inter- and intra-nationally

Removal of arsenic (V) from aqueous solutions using chitosan-red scoria and chitosan-pumice blends

In different regions across the globe, elevated arsenic contents in the groundwater constitute a major health problem. In this work, a biopolymer chitosan has been blended with volcanic rocks (red scoria and pumice) for arsenic (V) removal. The effect of three blending ratios of chitosan and volcanic rocks (1:2, 1:5 and 1:10) on arsenic removal has been studied. The optimal blending ratio was 1:5 (chitosan:volcanic rocks) with maximum adsorption capacity of 0.72 mg/g and 0.71 mg/g for chitosan:red scoria (Ch-Rs) and chitosan:pumice (Ch-Pu), respectively. The experimental adsorption data fitted well a Langmuir isotherm (R-2 > 0.99) and followed pseudo-second-order kinetics. The high stability of the materials and their high arsenic (V) removal efficiency (similar to 93%) in a wide pH range (4 to 10) are useful for real field applications. Moreover, the blends could be regenerated using 0.05 M NaOH and used for several cycles without losing their original arsenic removal efficiency. The results of the study demonstrate that chitosan-volcanic rock blends should be further explored as a potential sustainable solution for removal of arsenic (V) from water

The phage therapy paradigm: prêt-à-porter or sur-mesure?

The present opinion is the result of discussions on the future of phage therapy (personalized or large-scale uniform therapy?) during the first International Congress on Viruses of Microbes, held at the Institut Pasteur in Paris on June 21–25, 2010

The prognostic value of the hypoxia markers CA IX and GLUT 1 and the cytokines VEGF and IL 6 in head and neck squamous cell carcinoma treated by radiotherapy ± chemotherapy

BACKGROUND: Several parameters of the tumor microenvironment, such as hypoxia, inflammation and angiogenesis, play a critical role in tumor aggressiveness and treatment response. A major question remains if these markers can be used to stratify patients to certain treatment protocols. The purpose of this study was to investigate the inter-relationship and the prognostic significance of several biological and clinicopathological parameters in patients with head and neck squamous cell carcinoma (HNSCC) treated by radiotherapy ± chemotherapy. METHODS: We used two subgroups of a retrospective series for which CT-determined tumoral perfusion correlated with local control. In the first subgroup (n = 67), immunohistochemistry for carbonic anhydrase IX (CA IX) and glucose transporter-1 (GLUT-1) was performed on the pretreatment tumor biopsy. In the second subgroup (n = 34), enzyme linked immunosorbent assay (ELISA) was used to determine pretreatment levels of the cytokines vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6) in serum. Correlation was investigated between tumoral perfusion and each of these biological markers, as well as between the markers mutually. The prognostic value of these microenvironmental parameters was also evaluated. RESULTS: For CA IX and GLUT-1, the combined assessment of patients with both markers expressed above the median showed an independent correlation with local control (p = 0.02) and disease-free survival (p = 0.04) with a trend for regional control (p = 0.06). In the second subgroup, IL-6 pretreatment serum level above the median was the only independent predictor of local control (p = 0.009), disease-free survival (p = 0.02) and overall survival (p = 0.005). CONCLUSION: To our knowledge, we are the first to report a link in HNSCC between IL-6 pretreatment serum levels and radioresistance in vivo. This link is supported by the strong prognostic association of pretreatment IL-6 with local control, known to be the most important parameter to judge radiotherapy responses. Furthermore, the combined assessment of CA IX and GLUT-1 correlated independently with prognosis. This is a valuable indication that a combined approach is important in the investigation of prognostic markers

Oxygenated machine perfusion at room temperature as an alternative for static cold storage in porcine donor hearts

Background There is a continued interest in ex situ heart perfusion as an alternative strategy for donor heart preservation. We hypothesize that oxygenated machine perfusion of donor hearts at a temperature that avoids both normothermia and deep hypothermia offers adequate and safe preservation. Methods Cardioplegia-arrested porcine donor hearts were randomly assigned to six hours of preservation using cold storage (CS, n = 5) or machine perfusion using an oxygenated acellular perfusate at 21 degrees C (MP, n = 5). Subsequently, all grafts were evaluated using the Langendorff method for 120 min. Metabolic parameters and histology were analyzed. Systolic function was assessed by contractility and elastance. Diastolic function was assessed by lusitropy and stiffness. Results For both groups, in vivo baseline and post-Langendorff biopsies were comparable, as were lactate difference and myocardial oxygen consumption. Injury markers gradually increased and were comparable. Significant weight gain was seen in MP (p = 0.008). Diastolic function was not impaired in MP, and lusitropy was superior from 30 min up to 90 min of reperfusion. Contractility was superior in MP during the first hour of evaluation. Conclusion We conclude that the initial functional outcome of MP-preserved hearts was transiently superior compared to CS, with no histological injury post-Langendorff. Our machine perfusion strategy could offer feasible and safe storage of hearts prior to transplantation. Future studies are warranted for further optimization