Book Review: Telepractice in Audiology

This article presents a review of the book Telepractice in Audiology, authored by Emma Rushbrooke MPhil(AUD), BA, DipAud., MAudSA., LSLS. Cert. AVT, RNC, and K. Todd Houston, PhD, CCC-SLP, LSLS Cert. AVT, and 13 contributing authors.  This is the first book entirely devoted to tele-audiology. It provides practical information for working with clients across the lifespan and for multiple practice settings. Reviewer Dr. Barbara Vento endorses this work as a comprehensive resource on the topic of teleaudiology for both students and aspiring teleaudiologists.

Decoding human fetal liver haematopoiesis.

Definitive haematopoiesis in the fetal liver supports self-renewal and differentiation of haematopoietic stem cells and multipotent progenitors (HSC/MPPs) but remains poorly defined in humans. Here, using single-cell transcriptome profiling of approximately 140,000 liver and 74,000 skin, kidney and yolk sac cells, we identify the repertoire of human blood and immune cells during development. We infer differentiation trajectories from HSC/MPPs and evaluate the influence of the tissue microenvironment on blood and immune cell development. We reveal physiological erythropoiesis in fetal skin and the presence of mast cells, natural killer and innate lymphoid cell precursors in the yolk sac. We demonstrate a shift in the haemopoietic composition of fetal liver during gestation away from being predominantly erythroid, accompanied by a parallel change in differentiation potential of HSC/MPPs, which we functionally validate. Our integrated map of fetal liver haematopoiesis provides a blueprint for the study of paediatric blood and immune disorders, and a reference for harnessing the therapeutic potential of HSC/MPPs.We acknowledge funding from the Wellcome Human Cell Atlas Strategic Science Support (WT211276/Z/18/Z); M.H. is funded by Wellcome (WT107931/Z/15/Z), The Lister Institute for Preventive Medicine and NIHR and Newcastle-Biomedical Research Centre; S.A.T. is funded by Wellcome (WT206194), ERC Consolidator and EU MRG-Grammar awards and; S.B. is funded by Wellcome (WT110104/Z/15/Z) and St. Baldrick’s Foundation; E.L. is funded by a Wellcome Sir Henry Dale and Royal Society Fellowships, European Haematology Association, Wellcome and MRC to the Wellcome-MRC Cambridge Stem Cell Institute and BBSRC

Easy Does It: Advice on Writing a Successful Graduate School Application

Congratulations! You’ve chosen a rewarding career that will put you in the company of high caliber professionals. Application season is approaching, and the learning curve is sharp for completing a successful application. This article offers tips on making your application rise to the top, and helping you view this as a learning opportunity, not a dreaded chore

Easy Does It: Advice on Writing a Successful Graduate School Application

Congratulations! You’ve chosen a rewarding career that will put you in the company of high caliber professionals. Application season is approaching, and the learning curve is sharp for completing a successful application. This article offers tips on making your application rise to the top, and helping you view this as a learning opportunity, not a dreaded chore

Paleofloristic assemblage from the Paleogene Río Guillermo Formation, Argentina: preliminary results of phylogenetic relationships of Nothofagus in South America

Hünicken's paleobotanical collection is one of the most important in South America because it was the first one to be related to a documented stratigraphic profile. This floristic assemblage (Oligocene) recovered from Río Guillermo Formation, at Estancia Tres Marías, Río Guillermo Valley, Santa Cruz, Argentina, is described by the first time in this paper. Five species of genus Nothofagus (N. subferruginea, N. serrulata, N. crenulata, N. elongata and N. variabilis) together with Myrcia bagualense are components of the assemblage. The presence of Acaena brandmayri is confirmed for the first time for the area. Paleoenviromental and paleoclimate implications are also discussed. Characteristic species from a temperate-cold climate such as the genus Nothofagus are present in the material studied. A phylogenetic study of the fossil species herein described and the extant South American species of Nothofagus is presented. The preliminary results support the hypothesis that fossil species of Nothogafus are closely related to the modern species.Fil: Vento, Barbara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentina. Cornell University; Estados UnidosFil: Gandolfo, María A.. Cornell University; Estados UnidosFil: Nixon, Kevin Clark. Cornell University; Estados UnidosFil: Pramparo, Mercedes Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentin

Plio-Pleistocenic environment and displacement of the vegetation in the Payunia, Argentina

Los registros geocriogénicos fósiles del Plioceno-Pleistoceno se ubican a los 1650 m snm entre las localidades extraandinas de las sierras de Auca-Mahuida, Chachahuén, Volcán Payún Matru y Volcán Nevado y veinte andinas entre El Sosneado y puesto Las Lagunas (Neuquén). Actualmente los procesos geocriogénicos ocurren entre los 2900 y 4000 m snm. Se analizaron bioclimáticamente 17 localidades extraandinas y 20 andinas empleando los datos de temperatura media del mes más cálido y del más frío. Se calculó la ecuación de regresión que relaciona la temperatura en función de la altura. Se relevó fl orísticamente la vegetación en función de la altura. Se determinó la variación en altura de la línea de permafrost. Los procesos geocriogénicos actuales en la zona extraandina ocurrirían entre los 2600-3000 m snm y en la andina entre los 2900 y más de 4000 m snm. La vegetación acompañó este desplazamiento con el piso de tundra plio-pleistocénico ubicado a los 1500 m snm y actualmente por arriba de los 4000 m snm, bajo bioclima Mediteráneo subhúmedo por arriba de los 3500 m snm (Poetum durifoliae) y Semiárido superior e inferior a partir de los 1400 m snm (Adesmietum pinifoliae, Stillingio-Mulinetum, Retanillo-Colliguajetum, Fabiano-Stipetum, Adesmio-Prosopidastretum, Neospartetum aphylli, principalmente). De esta manera se concluye que ocurrió una variación altitudinal de 2000 m snm en un lapso de ca. 5 millones de años en la línea de permafrost y del piso de tundra desde el Plio-Pleistoceno a la actualidad, en un sistema de montaña cuya temperatura media mensual no superó los 10ºC.Fil: Martinez Carretero, Eduardo Enrique. Universidad Nacional de San Juan. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; ArgentinaFil: Vento, Barbara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; ArgentinaFil: Ripoll, Y.. Universidad Nacional de San Juan. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; ArgentinaFil: Ontivero, Marcela del Valle. Universidad Nacional de San Juan. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; ArgentinaFil: Herrera Moratta, Mario Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina. Universidad Nacional de San Juan; ArgentinaFil: Navas, A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina. Universidad Nacional de San Juan; ArgentinaXXXVII Jornadas Argentinas de BotánicaTucumanArgentinaSociedad Argentina de Botanic

Single-cell reconstruction of the early maternal-fetal interface in humans.

During early human pregnancy the uterine mucosa transforms into the decidua, into which the fetal placenta implants and where placental trophoblast cells intermingle and communicate with maternal cells. Trophoblast-decidual interactions underlie common diseases of pregnancy, including pre-eclampsia and stillbirth. Here we profile the transcriptomes of about 70,000 single cells from first-trimester placentas with matched maternal blood and decidual cells. The cellular composition of human decidua reveals subsets of perivascular and stromal cells that are located in distinct decidual layers. There are three major subsets of decidual natural killer cells that have distinctive immunomodulatory and chemokine profiles. We develop a repository of ligand-receptor complexes and a statistical tool to predict the cell-type specificity of cell-cell communication via these molecular interactions. Our data identify many regulatory interactions that prevent harmful innate or adaptive immune responses in this environment. Our single-cell atlas of the maternal-fetal interface reveals the cellular organization of the decidua and placenta, and the interactions that are critical for placentation and reproductive success.Wellcome Trust Centre for Trophoblast Research Human Frontier Science Program Long-Term Fellowship EMBO Long-Term Fellowship Royal Society Dorothy Hodgkin Fellowshi

Top-down proteomics for the investigation of saliva during human development

Aim of this study was to evaluate by a label-free top-down proteomic platformsvariations during human development of the level of different proteins detectable in adult saliva and their post-translational modifications (PTMs). The relative amounts of different proteoforms of acidic-proline-rich proteins (aPRPs), basic proline-rich proteins, statherin, histatins 1, 3, 5 and 6, cystatin S, S1, S2, SA and SN and S100A9 were measured in 312 samples (111 serially collected from 17 preterm newborns at different post-conceptional age (PCA), 178 from subjects aged between 0 and 18 years, and 23 from adults). The different proteoforms of these protein families were expressed according to this order: aPRPs(PRH2 locus[1]) and g-PRP(PRB3 locus)before 195 days of PCA; histatin 1 and statherin at 210-220 days of PCA; aPRPs (PRH1 locus)at normal term of delivery (270-280 days of PCA); cystatin S proteoforms after normal term of delivery;bPRPs (PRB1, PRB2 and PRB4 loci) later, reaching adult levels after puberty [2].All S100A9 proteoformswere highly abundant in pre-term newborn saliva, their concentration decreasing as a function of PCA [3]. The PTMsevaluation of various proteoforms highlighted that the Golgi casein kinase (Fam20C [4]), responsible for the aPRPs, histatin 1, statherin and cystatin Sphosphorylation, is poorly active during fetal development. The p38 mitogen-activated protein kinase 14(MAPK14) responsible for S100A9 phosphorylation, the convertases, thecarboxy-peptidases, themethionine aminopeptidasesand N-terminal-acetyl-transferasesresponsible for the cleavage and processing of different proteins (aPRPs, bPRPs, histatins, S100A9) during secretionare fully active before 195 days of PCA

A roadmap for the Human Developmental Cell Atlas

The Human Developmental Cell Atlas (HDCA) initiative, which is part of the Human Cell Atlas, aims to create a comprehensive reference map of cells during development. This will be critical to understanding normal organogenesis, the effect of mutations, environmental factors and infectious agents on human development, congenital and childhood disorders, and the cellular basis of ageing, cancer and regenerative medicine. Here we outline the HDCA initiative and the challenges of mapping and modelling human development using state-of-the-art technologies to create a reference atlas across gestation. Similar to the Human Genome Project, the HDCA will integrate the output from a growing community of scientists who are mapping human development into a unified atlas. We describe the early milestones that have been achieved and the use of human stem-cell-derived cultures, organoids and animal models to inform the HDCA, especially for prenatal tissues that are hard to acquire. Finally, we provide a roadmap towards a complete atlas of human development