Agrobacterium-mediated transformation of White Ponni, a non-basmati variety of indica rice (Oryza sativa L.)

We report successful Agrobacterium-mediated transformation of a popular rice variety White Ponni, a non-basmati indica rice (Oryza sativa L.). Scutellumderived calluses of White Ponni were transformed with Agrobacterium strain LBA4404 (pSB1) harbouring the binary vector pMKU-RF2 with rice chi11 gene. Five independent transgenic White Ponni plants were generated from hygromycin-resistant calluses. Stable integration of the transgene was confirmed and copy numbers were determined by Southern analysis. Among the five plants, four possessed single-copy T-DNA integration events while one was found to have two integrated copies of T-DNA. Western analysis revealed a higher level of chitinase accumulation in all the five T0 plants. Progeny analysis of T0 plants confirmed the inheritance of the transgene to the next generation

The current status of plant transformation technologies

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Kanamycin sensitivity of cultured tissues of Piper nigrum L.

The kanamycin sensitivity for callus growth was studied in vitro in a cultivar of black pepper (Piper nigrum) using cotyledons as explants to investigate the suitability of kanamycin resistance as a selectable marker for Agrobacterium mediated transformation. Callus formation was completely inhibited at 50 ug ml-1 and above concentrations of kanamycin suggesting that 50 ug ml-1 is the minimum concentration needed to select the transformed tissues. &nbsp

Kanamycin sensitivity of cultured tissues of Piper nigrum L.

The kanamycin sensitivity for callus growth was studied in vitro in a cultivar of black pepper (Piper nigrum) using cotyledons as explants to investigate the suitability of kanamycin resistance as a selectable marker for Agrobacterium mediated transformation. Callus formation was completely inhibited at 50 ug ml-1 and above concentrations of kanamycin suggesting that 50 ug ml-1 is the minimum concentration needed to select the transformed tissues. &nbsp

Opines stimulate induction of the vir genes of the Agrobacterium tumefaciens Ti plasmid.

Upon incubation of Agrobacterium tumefaciens A348 with acetosyringone, the vir genes encoded by the Ti (tumor-inducing) plasmid are induced. The addition of certain opines, including octopine, nopaline, leucinopine, and succinamopine, enhanced this induction 2- to 10-fold. The compounds mannopine, acetopine, arginine, pyruvate, and leucine did not stimulate the induction of the vir genes to such an extent. The enhancement of vir gene induction by opines depended on acetosyringone and the genes virA and virG. Opines stimulated the activity of the vir genes, the double-stranded cleavage of the T (transferred)-DNA at the border repeat sequences, and the production of T-strands by the bacterium. The transformation efficiency of cotton shoot tips was markedly increased by the addition of acetosyringone and nopaline at the time of infection

Dimerization of VirD2 Binding Protein Is Essential for Agrobacterium Induced Tumor Formation in Plants

10.1371/journal.ppat.1003948PLoS Pathogens103

The Influence of Rapid Transit Systems on Condominium Prices in Bangkok : A Hedonic price model approach

Rapid transit systems often create city developments and raise the property values. Basically, residential property price is characterised by number of characteristics including the transportations attributes. Empirical studies have drawn impacts of transportation in different ways. This thesis studies the hedonic price model to better understand the influence of mass rapid transit systems on the prices of condominium in Bangkok. The research question is "How the accessibility of rapid transit system affects the price of condominium in Bangkok?" The thesis explains the relationship between condominium prices and accessibility to mass rapid transit systems, as well as other influences, using hedonic price model. The research conducts cross-sectional study approach that uses the data with high level of flexibility at a particular point in time. Five models have been used in this thesis including the entire area model, models of proximity to rapid transit station, and sub-area models. Consequently evidences from the entire area model indicate that proximity to the rapid transit systems has a significant negative effect on condominium prices. Besides, the research also points out number of important characteristics that influence the prices of condominium including distance to main street and public facilities in the area. However, the clustered models show that there is no significant effect of proximity to mass rapid transits on condominium prices. The thesis has raised the knowledge and gained better understanding of the hedonic price model especially its application to the property market. The findings of this thesis can lead to an analysis of property values as well as other price model in other field of study