Desenvolvimento inicial do arroz com a antecipação da adubação de nitrogênio e seu efeito na produtividade do arroz no município de Paragominas, PA.

Analisou-se a resposta do arroz de terras altas cultivado em Latossolo Amarelo distrofico na região Nordeste Paraense, ao parcelamento e épocas de aplicação de fertilizante nitrogenado. Observou-se também as formas que permitam adaptar o sistema de produção do arroz de terras altas ao plantio direto de forma a permitir que faca parte de sistemas agrícolas em rotação com soja, adequando ao manejo de adubação, a relação de formas de N no solo, aos tipos e época de manejo das coberturas de solo e a qualidade de semeadura

Free mobility, locked rights: the posting of construction workers from Portugal

Mobility has become a cornerstone of the contemporary social world. In the European Union, the removal of barriers aimed at promoting the free movement of the labour force is a major goal of the integration process. This article investigates the political economy of posting, analysing the working and living conditions of Portuguese construction workers posted to other EU Member States. It highlights a set of abusive and exploitative practices involved in posting and the role those practices play in the (de)regulation of labour within the construction sector. In spite of the access to a pan-European labour market and the general principle of equal treatment, posted workers have restricted social rights in the host country. The study presented is based on research developed within the ‘EU Post Lab’ project, aimed at promoting activities of cooperation and awareness raising in the field of posting. The methodology adopted includes document analysis and interviews.info:eu-repo/semantics/acceptedVersio

Country-level findings: Portugal

info:eu-repo/semantics/publishedVersio

Implementation of a specific rate controller in a fed-batch E. coli fermentation

The specific growth rate is one of the most important process variables characterizing the state of microorganisms during fermentations mainly because the biosynthesis of many products of interest is often related with the values assumed by this parameter. In the particular case of the fed-batch operation of Escherichia coli for the production of recombinant proteins, it is important to maintain the specific growth rate below a certain threshold in order to avoid the accumulation of acetic acid throughout the fermentation and, additionally, it is often argued that both pre- and the post-induction specific growth rates should be closely controlled in order to achieve maximum productivities of the desired recombinant protein. In a previous work the authors have developed and validated by simulations a strategy for the automatic control of the specific growth rate in E. coli fed-batch fermentations based on an asymptotic observer for biomass and on developed estimators for the specific growth rates. The main purpose of the present work was to implement experimentally the developed observer, estimator and controller in a real fed-batch fermentation process. For that purpose a data acquisition and control program was developed in LabVIEW that allows the acquisition of the necessary on line data (off gas and dissolved oxygen concentration and culture weight) and the calculation of the feeding rates using the developed equations. The feedforward-feedback controller developed was able to keep the culture growing in an exponential phase throughout the fermentation without accumulation of glucose and acetate.Fundação para a Ciência e a Tecnologia (FCT) - RecSysBio projecto POCI/BIO/60139/200

Amostragem de solo para análise química.

Publicado também como folder (FD 00709).bitstream/item/27662/1/com.tec.131.pdfDisponível também on-line

Identificação e controle das principais pragas em pomares de citros no Pará.

Publicado também como folder (FD 00715).bitstream/item/28007/1/com.tec.119.pdfDisponível também on-line

Application of genome-scale metabolic models to the optimization of recombinant protein production in Escherichia coli

Escherichia coli has been the organism of choice for the production of many recombinant proteins with high therapeutic value. However, while the research on molecular biology has allowed the development of very strong promoters, there are still some phenomena associated with this process that hamper the full use of those technologies: aerobic acetate production associated with high specific growth rates, and the so-called stringent response that usually occurs when very high levels of heterologous protein production takes place. In both cases, productivity is affected due to a decrease in the specific growth and production rates. In this work, a systems biology approach for modelling recombinant protein production processes was used aiming its optimization. The existing genome-scale metabolic model of Escherichia coli was modified by including an equation for protein production (the model protein GFP – Green Florescent Protein), based on its amino acids content. For the validation of the genome-scale model in high-cell density processes, highly reproducible fed-batch fermentations are run with constant specific growth rate. The developed data acquisition and control system allows to control the substrate addition rate, and to acquire on-line the fermenter’s weight, to calculate oxygen and carbon dioxide transfer rates, as well as to obtain glucose and acetate concentrations using a developed Flow Injection Analysis system

Ocorrencia da cigarrinha Molomea cincta (Signoret, 1854) em mangueira, na microrregiao de Teresina-PI.

bitstream/item/83446/1/CT940001.pd

Quantification of the total suspended matter concentration in the sea breaking zone from in situ measurements and remotely sensed data - two empirical approaches

Remote sensing techniques can be used to calculate suspended sediment concentrations and to understand the flux and distribution of sediments driven by mechanisms such as tides and waves, river discharges, etc. The main objective of this study is the quantification of the Total Suspended Matter (TSM) concentration in the sea breaking zone for a particular area of the Portuguese coast, around Aveiro. The methodology used was based on in situ measurements and multi spectral satellite images. In situ experimental techniques (maritime platform, aerial platform, simulation on the beach and water sample collection in the breaking zone) were used to determine a relationship between the TSM concentration and the seawater reflectance in the breaking zone. Spectral reflectance was measured with a spectroradiometer and water samples were simultaneously collected. Empirical relationships were established between TSM concentration and the equivalent reflectance values for sensors SPOT/HRVIR, TERRA/ASTER and Landsat/TM at visible and Near Infra Red (NIR) bands computed from the experimental data. Satellite images from ASTER, SPOT HRVIR and Landsat TM were used together with the same empirical models. These satellite images were calibrated and atmospherically corrected. Equations of linear, polynomial, logarithmic, power and exponential models were tested for the satellite image bands on the visible and near infrared. The coefficients of determination (R-2) were also calculated for each model. The results obtained from the two approaches, in situ measurements and directly from the multi spectral satellite images, were analysed