7 research outputs found
A Prospective Three-Year Cohort Study of the Epidemiology and Virology of Acute Respiratory Infections of Children in Rural India
Acute respiratory infection (ARI) is a major killer of children in developing countries. Although the frequency of ARI is similar in both developed and developing countries, mortality due to ARI is 10-50 times higher in developing countries. Viruses are common causes of ARI among such children, yet the disease burden of these infections in rural communities is unknown.A prospective longitudinal study was carried out in children enrolled from two rural Indian villages at birth and followed weekly for the development of ARI, classified as upper respiratory infection, acute lower respiratory infection (ALRI), or severe ALRI. Respiratory syncytial virus (RSV), influenza, parainfluenza viruses and adenoviruses in nasopharyngeal aspirates were detected by direct fluorescent antibody testing (DFA) and, in addition, centrifugation enhanced culture for RSV was done. 281 infants enrolled in 39 months and followed until 42 months. During 440 child years of follow-up there were 1307 ARIs, including 236 ALRIs and 19 severe ALRIs. Virus specific incidence rates per 1000 child years for RSV were total ARI 234, ALRI 39, and severe ALRI 9; for influenza A total ARI 141, ALRI 39; for INF B total ARI 37; for PIV1 total ARI 23, for PIV2 total ARI 28, ALRI 5; for parainfluenza virus 3 total ARI 229, ALRI 48, and severe ALRI 5 and for adenovirus total ARI 18, ALRI 5. Repeat infections with RSV were seen in 18 children.RSV, influenza A and parainfluenza virus 3 were important causes of ARI among children in rural communities in India. These data will be useful for vaccine design, development and implementation purposes
DFA for detection of respiratory viruses in ARI
<p>DFA for detection of respiratory viruses in ARI</p
Incidence rates of ARIs in the study population by age
<p>Note. Incidence rate (IR) per 1000 child years and 95% Confidence Intervals (CI)</p
Demographics and disease severity with repeat infections by RSV
<p>Note: virus detection by DFA or, for RSV, DFA and CEC.</p><p>ARI classification of disease per WHO <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000491#pone.0000491-WHO2" target="_blank">[9]</a>.</p
Monthly distribution of viral identification throughout study period October 2001 to March 2005.
<p>RSV was identified by DFA and CEC, INF and PIV by DFA. Total positive represents the total number of samples with a virus identified. Panel A, RSV; panel B, influenza A and B; panel C, parainfluenza 1, 2, and 3.</p
Severity of ARI and incidence rates for viruses by age groups
<p>Note. RSV detection by DFA and CEC, INF A and PIV 3 detected by DFA alone. Incidence rate (IR) per 1000 child years.</p