Case Report OPEN ACCESS Overexpression of linker for activated T cells, cyclooxygenase-2, CD1a, CD68 and myeloid/histiocyte antigen in an inflamed seborrheic keratosis

Abstract Context: Inflamed seborrheic keratoses are generally associated with the accumulation of variable numbers of lymphocytes and histiocytes in the superficial dermis. The precise immunologic mechanism of this histologic phenomenon is not known Case Report: A 62-year-old male presented with a patch on the right neck with additional features of inflammation. Skin biopsies for hematoxylin and eosin examination, as well as for immunohistochemistry analysis were performed. Results: H&E staining demonstrated classic features of an inflamed seborrheic keratosis. Overexpression of LAT, COX-2, CD1a, and CD68 was noticed in the inflammatory infiltrate. A strong presence of CD1a was also seen in the epidermis suprajacent to the inflammation. Myeloid/histiocyte antigen was strongly expressed by the keratinocytes. Conclusion: A complex immune response seems to be involved in the pathophysiology of an inflamed seborrheic keratosis

“TEACHING LAB” AS AN EDUCATIONAL PROPOSAL IN THE INITIAL TRAINING OF GEOGRAPHY TEACHERS

Considering the requests from students and the identified needs in the initial training of teachers in general, and Geography in particular, during this year it has taken place the “Teaching Lab”, for undergraduates of the first year of the Masters in Geography Teaching in the 3rd cycle and Secondary Teaching of the Faculty of Arts and Humanities of the University of Coimbra (Portugal).  Article visualizations

Patrones de producción y consumo responsable: La carrera por el logro de los Objetivos de Desarrollo Sostenible (ODS) en mercados emergentes

Since the end of the 20th century, the role of private multinational enterprises (MNEs) has been recognized as critical in implementing increased sustainable production and consumption atterns. Particularly after the creation of the Sustainable Development Goals (SDGs) and the Agenda 2030, this role has increased. In this sense, this paper aims to analyze the measures and actions taken by companies in their contribution to the achievement of the SDG 12. Through the identification of more than 52 metrics in sustainability reports of 854 firms, findings suggest that direct greenhouse gas emissions and indirect greenhouse gas emissions are the most often reported corporate metrics to measure their impact on specific SDGs. This reveals the importance of sustainability actions in emerging market firms as a mechanism to gain legitimacy when operating in foreign markets and as an opportunity to create more sustainable production models.Desde finales del siglo XX, se ha reconocido que el papel de las empresas multinacionales (EMN) privadas es fundamental en el proceso de implementación de patrones de producción y consumo más sostenibles. Especialmente, tras la creación de los Objetivos de Desarrollo Sostenible (ODS) y la Agenda 2030, este papel ha aumentado. En este sentido, este trabajo tiene como objetivo analizar las medidas y acciones tomadas por las empresas en su contribución al logro del ODS 12. Mediante la identificación de más de 52 métricas en los informes de sostenibilidad de 854 empresas, los hallazgos sugieren que las emisiones directas de gases de efecto invernadero y las emisiones indirectas de gases de efecto invernadero son las métricas corporativas con más información para medir su impacto en ODS específicos. Esto revela la importancia de las acciones de sostenibilidad en las empresas de mercados emergentes como mecanismo para ganar legitimidad al operar en mercados externos y como oportunidad para la creación de modelos de producción más sostenibles

Urban Sharing in Shanghai

This city report is the result of a Mobile Research Lab conducted online in Shanghai during spring 2020. The Mobile Research Lab involves a combination of methods, including case studies, interviews, observations, expert panels, and in-situ field work. This report presents insights gained by the Urban Sharing research team Oksana Mont (PI), Andrius Plepys, Yuliya Voytenko Palgan, Matthias Lehner, Steven Curtis, Lucie Zvolska and Ana Maria Arbelaez Velez

Immunologic findings in central centrifugal cicatricial alopecia

Introduction: Premature desquamation of the inner root sheath is described as a defining histologic feature of follicular degeneration syndrome/central centrifugal cicatricial alopecia; moreover, the immunological features of this disease are not well established.Case report: A 46-year-old African American female was evaluated for an asymptomatic scarring alopecia after using several chemicals on her hair. The clinical examination revealed visible, well defined patches of hair loss.Methods: Biopsies for hematoxylin and eosin examination, as well as for direct immunofluorescence and immunohistochemistry analysis were performed. We evaluated molecules involved in signaling of growth factor pathways (e.g. the Akts), specifically VEGF and Oct-4 to investigate involvement of these molecules in this disease. Hematoxylin and eosin staining demonstrated histopathologic findings of premature desquamation of the inner root sheath and eccentric thinning of the follicular epithelium, supporting the diagnosis of central centrifugal cicatricial alopecia. Direct immunofluorescence revealed strong depositions of IgG, Complement/C3 and fibrinogen around the multiple hair follicles and their supply vessels. Immunohistochemistry staining of the base of the hair follicle was seen with fibrinogen and Oct-4 antibodies. Immunohistochemistry also demonstrated increased expressions of VEGF around supply vessels of the hair follicle, as well as some overexpression of anti-human Akt-pS473 phosphorylation site specific antibody.Conclusions: Our immunologic findings suggest that the etiology of centrifugal cicatricial alopecia includes not only hair traction, but also a possible reactive immune response

Low-risk polycythemia vera treated with phlebotomies: clinical characteristics, hematologic control and complications in 453 patients from the Spanish Registry of Polycythemia Vera

Myelofibrosis; Phlebotomies; ThrombosisMielofibrosi; Flebotomies; TrombosiMielofibrosis; Flebotomías; TrombosisHematological control, incidence of complications, and need for cytoreduction were studied in 453 patients with low-risk polycythemia vera (PV) treated with phlebotomies alone. Median hematocrit value decreased from 54% at diagnosis to 45% at 12 months, and adequate hematocrit control over time ( 60 years, and microvascular symptoms constituted the main indications for starting cytoreduction. Median duration without initiating cytoreduction was significantly longer in patients younger than 50 years (< 0.0001). The incidence rate of thrombosis under phlebotomies alone was 0.8% per year and the estimated probability of thrombosis at 10 years was 8.5%. The probability of arterial thrombosis was significantly higher in patients with arterial hypertension whereas there was a trend to higher risk of venous thrombosis in cases with high JAK2V617F allele burden. Rates of major bleeding and second primary neoplasm were low. With a median follow-up of 9 years, survival probability at 10 years was 97%, whereas the probability of myelofibrosis at 10 and 20 years was 7% and 20%, respectively. Progression to acute myeloid leukemia was documented in 3 cases (1%). Current management of low-risk PV patients is associated with low rate of thrombosis and long survival. New treatment strategies are needed for improving hematological control and, in the long term, reducing progression to myelofibrosis.This work was supported by PI18/01472, PI18/00205, and PI21/00231 from the Instituto de Salud Carlos III (ISCIII), through the Plan Estatal de Investigación Científica y Técnica y de Innovación. GEMFIN received a grant from Novartis for the development of the Spanish Registry of Polycythemia Vera and for conducting the present project

LAT, EGFR -pY197, PCNL2, CDX2, HLA-DPDQDR, bromodeoxyuridine, JAM-A, and ezrin immunoreactants in a rubbed spongiotic dermatitis

Background: Acute and subacute spongiotic dermatitides are among the most commonly diagnosed types of dermatitis. Many patients rub their lesions, with the lesions becoming clinically thickened. The precise immunologic mechanisms within the thickening process are not well defined. Case report: An 85 year old male presented with the sudden clinical appearance of erythematous patches and small blisters on the back of his legs, with pruritis. Methods: Skin biopsies, one from a rubbed lesion and one from a non-rubbed lesion were submitted for hematoxylin and eosin (H&E), immunohistochemistry (IHC), and for direct immunofluorescence (DIF) analysis. Results: The H&E staining demonstrated classic features of a spongiotic dermatitis, but in the rubbed areas psoriasiform hyperplasia was also seen. The psoriasiform areas demonstrated positive, focal IHC staining with bromodeoxyuridine, LAT, EGFR-pY197, PCNL2, CDX2, and HLA-DPDQDR antibodies. DIF staining revealed positive staining of JAM-A and ezrin in the non-rubbed specimens in both the spongiotic epidermis and in the adjacent vessels; normal expression of these markers was appreciated in the rubbed biopsy. Conclusions: The immune response seems to be complex when a spongiotic dermatitis is converted from a non-rubbed to a rubbed lesion with histologic features of psoriasiform hyperplasia

Upregulation of anti-human ribosomal protein S6-p240, topoisomerase II ά, cyclin D1, Bcl-2 and anti-corneal antibodies in acute psoriasis

Background. The immunopathogenesis of psoriasis is complex, and involves alterations in the innate immunologic system Case Report: A 57-year-old female was evaluated for the presence of rapidly appearing plaques on the knees and elbows. Methods: Skin biopsies for hematoxylin and eosin examination, as well as for direct immunofluorescence and immunohistochemistry analysis were performed. Results: H&E examination demonstrated classic features of psoriasis. Direct immunofluorescence revealed positive anti-corneal antibodies with several immunoglobulins, as well as positivity to upper and deep small dermal blood vessels. Immunohistochemistry revealed an increased expression of survivin, anti-human-ribosomal protein S6-p240, Topoisomerase II ά, cyclin D1, and Bcl-2 in lesional plaques. Conclusions: The pathobiology of psoriasis seems to involve a series of molecules involved in a complex interaction between the inflammation itself, cell cycle regulation, and ectopic expression of selected molecules

Automated Design of Common-Mode Suppressed Balanced Wideband Bandpass Filters by Means of Aggressive Space Mapping

The automated and unattended design of balanced microstrip wideband bandpass filters by means of aggressive space mapping (ASM) optimization is reported in this paper. The proposed filters are based on multisection mirrored stepped impedance resonators (SIRs) coupled through quarter-wavelength transmission lines, acting as admittance inverters.This work was supported by MINECO-Spain (projects TEC2013-47037-C5-1-R, TEC2013-40600-R, TEC2013-49221-EXP), Generalitat de Catalunya (project 2014SGR-157), Institucio Catalana de Recerca i Estudis Avancats (who awarded Ferran Martin), and by FEDER funds.Sans, M.; Selga, J.; Velez, P.; Rodriguez Perez, AM.; Bonache Albacete, J.; Boria Esbert, VE.; Martin, F. (2015). Automated Design of Common-Mode Suppressed Balanced Wideband Bandpass Filters by Means of Aggressive Space Mapping. IEEE Transactions on Microwave Theory and Techniques. 63(12):3896-3908. https://doi.org/10.1109/TMTT.2015.2495180S38963908631