Evaluation of caraway essential oil from different production areas of Serbia

Caraway fruits contain essential oils that give caraway its characteristic aroma. This paper evaluates quantity and composition of the essential oil from the annual caraway grown in different production areas of Serbia. Experiments were conducted in three production areas: Banat, Backa and Srem, during two years. The fruits contained 3.95% of oil on average. The highest content of essential oil was obtained in the production area of Banat, a part of Serbia where environmental conditions are very favourable for cultivation of medicinal plants. Weather conditions have not a significant influence on the content of essential oil in caraway fruits. However, weather conditions as well as production region had a great influence on seed yield and essential oil yield. There were 22 compounds determined in caraway essential oil by GC/MS. The main components were carvone and limonene, whose mixture constituted from 97.69 to 98.62% of total oil composition. Carvone/limonene ratio was 0.58% on average, therefore, it can be concluded that this annual caraway belongs to limonene chemotype

Compositional characteristics of the essential oil of Pimpinella anisum and Foeniculum vulgare grown in Serbia

The essential oils obtained by hydrodistillation from the mature fruits of Pimpinella anisum and Foeniculum vulgare were analyzed by GC-MS. The oil of aniseed was characterized by higher amounts of trans-anethole (96.80%) than fennel essential oil (83.43%). The isomer of trans-anethole, methyl chavicol (estragol) was also present in both plants, with 1.36% in fennel and 0.19% in aniseed. Apart from these two phenylpropanoids, nine sesquiterpenes and one monoterpene were present in essential oil of Pimpinella anisum, which constituted the other 3.01% of essential oil, but only γ-himachalene was present with more than 1%. In the case of fennel, eight monoterpenes constituted the other 15.21% of essential oil, and limonene and fenchone were present with contributions of 9.34 and 4.58%, respectively. © 2015 Institute of Botany and Botanical Garden Jevremovac, Belgrade

Supplementary data for the article: Aćimović, M.; Pezo, L.; Tešević, V.; Čabarkapa, I.; Todosijević, M. QSRR Model for Predicting Retention Indices of Satureja Kitaibelii Wierzb. Ex Heuff. Essential Oil Composition. Industrial Crops and Products 2020, 154, 112752. https://doi.org/10.1016/j.indcrop.2020.112752

Supplementary material for: [https://doi.org/10.1016/j.indcrop.2020.112752]Related to published version: [https://cherry.chem.bg.ac.rs/handle/123456789/4209

Sadržaj i hemijski sastav etarskog ulja anisa

The field experiments were carried out during 2011 and 2012 in three localities in Vojvodina (Serbia) with the application of six different fertilizer regimes aimed at determining the content and composition of the aniseed essential oil. It was found that the average essential oil content of aniseed, obtained by hydrodistillation, was 3.72%. The weather conditions during the year and the locality had a statistically significant effect on the essential oil content, while different source of fertilizers was not statistically significant for the essential oil content and its composition. Essential oil composition was determined using GC–MS technique, and a total of 15 compounds were identified. It was found that the major component was trans-anethole, 94.78% on the average, and the coefficient of variation was 2%. The second most abundant component was γ-himachalene with 2.53% (CV 28%). All other components were present in less than 1%.U radu su prikazani rezultati ispitivanja količine i hemij- skog sastava etarskog ulja anisa gajenog tokom 2011. i 2012. godine na tri lokaliteta u Vojvodini (Srbija), pri primeni šest različitih vrsta đubriva. Utvrđeno je da je prosečna količina etarskog ulja u plodovima anisa dobijena metodom de stilacije vodenom parom 3.72%. Vremenski uslovi tokom godine i lokalitet imaju statistički značajan efekat na sadržaj etarskog ulja i njegov hemijski sa stav, dok primena različitih vrsta đubriva ne utiče na ove parametre. Hemij ski sastav etarskog ulja određen je primenom GC–MS analize, pri čemu je identifikovano ukupno 15 komponenti. Ustanovljeno je da je glavna komponenta transanetol, se prosečno 94,78%, i koeficijentom varijacije 2%. Druga najzastupljenija komponenta bila je γ-himahalen sa 2,53% (CV 28%). Sve ostale komponente bile su prisutne sa manje od 1%

Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of Cotinus Coggygria Scop. Against Breast and Colon Cancer Cell Lines

Background and objective: Cotinus coggygria Scop. is a valuable medicinal plant species with pronounced pharmacological potential due to its numerous biological activities. The herb is characterized by a high content of polyphenols among which is fustin. The anticancer activities of fustin, however, are extremely weakly studied. The aim of the present study was to investigate the in vitro antiproliferative potential of fustin isolated from the heartwood of C. coggygria against cell lines originating from two of the most common cancer types – breast (MDA-MB-231 and MCF7), and colon cancer (Colon 26). Materials and methods: Cell growth inhibitory properties of fustin were examined by MTT assay. Subsequently, phase-contrast and fl uorescence microscopy analysis as well as colonyforming assay were carried out on the most sensitive to the cytostatic action of the fustin cell line. Results: The obtained results showed that fustin reduced the proliferation of all studied cell lines. The highest cytostatic eff ect was registered towards breast cancer MDA-MB-231 cells with a half maximal inhibitory concentration (IC50) value of 56.02 μg/ ml followed by colon cancer cells with an IC50 of 78.07 μg/ml. MCF7 cell proliferation was least aff ected with a calculated IC50 of 187.8 μg/ml. Further investigations on breast cancer MDA-MB-231 cells indicated decreased density of cell monolayer and some morphological alterations, signifi cant attenuation in the number of viable cells, and diminished clonogenic ability of cells after fustin exposure. Conclusion: It could be concluded that fustin isolated from the heartwood of medicinal plant C. coggygria possesses marked antiproliferative properties against breast cancer cell line MDA-MB-231 which will be a subject of our more detailed future investigations

Bioactivity of essential oils from cultivated winter savory, sage and hyssop

Species of the Lamiaceae family have enjoyed a rich tradition of use for flavoring, food preservation, and medicinal purposes, due to their curative and preventive properties. Cultivated winter savory (Satureja montana L.), sage (Salvia officinalis L.) and hyssop (Hyssopus officinalis L.) are produced for seed, herb, and essential oil. Dominant compounds in S. montana essential oil were carvacrol (43.2%) and thymol (28.4%), while CIS-thujone (27.1%) and camphor (19.3%), followed by trans-thujone and 1,8-cineole were the major compounds in S. officinalis essential oil. As for H. officinalis essential oil, CIS-and trans-pinocamphone (41.1% and 20.5%, respectively) were the most abundant compounds, followed by b-pinene. S. montana essential oil exhibit the highest antimicrobial properties, as well as antioxidant capacity, compared to other tested essential oils. Furthermore, H. officinalis essential oils showed higher antioxidant activity than that of S. officinalis. The aim of this investigation was to determine the composition and bioactivity of essential oils of mentioned varieties. Presented results show that S. montana essential oil could be proposed as a valuable source of natural preservatives

Compositional characteristics of the essential oil of Pimpinella anisum and Foeniculum vulgare grown in Serbia

The essential oils obtained by hydrodistillation from the mature fruits of Pimpinella anisum and Foeniculum vulgare were analyzed by GC-MS. The oil of aniseed was characterized by higher amounts of trans-anethole (96.80%) than fennel essential oil (83.43%). The isomer of trans-anethole, methyl chavicol (estragol) was also present in both plants, with 1.36% in fennel and 0.19% in aniseed. Apart from these two phenylpropanoids, nine sesquiterpenes and one monoterpene were present in essential oil of Pimpinella anisum, which constituted the other 3.01% of essential oil, but only γ-himachalene was present with more than 1%. In the case of fennel, eight monoterpenes constituted the other 15.21% of essential oil, and limonene and fenchone were present with contributions of 9.34 and 4.58%, respectively. © 2015 Institute of Botany and Botanical Garden Jevremovac, Belgrade

Weather Conditions Influence on Lavandin Essential Oil and Hydrolate Quality

Lavandula sp. essential oil and hydrolate are commercially valuable in various industry branches with the potential for wide-ranging applications. This study aimed to evaluate the quality of these products obtained from L. x intermedia cv. ‘Budrovka’ for the first time cultivated on Fruška Gora Mt. (Serbia) during three successive seasons (2019, 2020, and 2021). Essential oil extraction was obtained by steam distillation, and the composition and influence of weather conditions were also assessed, using flowering tops. The obtained essential oils and hydrolates were analysed by gas chromatography with a flame ionization detector (GC-FID) and gas chromatography coupled to mass spectrometry (GC-MS). A linear regression model was developed to predict L. x intermedia cv. ‘Budrovka’ essential oil volatile compound content and hydrolate composition during three years, according to temperature and precipitation data, and the appropriate regression coefficients were calculated, while the correlation analysis was employed to analyse the correlations in hydrolate and essential oil compounds. To completely describe the structure of the research data that would present a better insight into the similarities and differences among the diverse L. x intermedia cv. ‘Budrovka’ samples, the PCA was used. The most dominant in L. intermedia cv. ‘Budrovka’ essential oil and hydrolate were oxygenated monoterpenes: linalool, 1,8-cineole, borneol, linalyl acetate, and terpinene-4-ol. It is established that the temperature was positively correlated with all essential oil and hydrolate compounds. The precipitations were positively correlated with the main compounds (linalool, 1,8-cineole, and borneol), while the other compounds’ content negatively correlated to precipitation. The results indicated that Fruška Gora Mt. has suitable agro-ecological requirements for cultivating Lavandula sp. and providing satisfactory essential oil and hydrolate. © 2022 by the authors. Licensee MDPI, Basel, Switzerland

Antifungal and antibacterial activities of Petroselinum crispum essential oil

Parsley [Petroselinum crispum (Mill.) Fuss] is regarded as an aromatic, culinary, and medicinal plant and is used in the cosmetic, food, and pharmaceutical industries. However, few studies with conflicting results have been conducted on the antimicrobial activity of parsley essential oil. In addition, there have been no reports of essential oil obtained from parsley aerial parts, except seeds, as an alternative natural antimicrobial agent. Also, microorganism resistance is still a challenge for health and food production. Based on the demand for natural products to control microorganisms, and the reevaluation of potential medicinal plants for controlling diseases, the objective of this study was to determine the chemical composition and antibacterial and antifungal activities of parsley essential oil against foodborne diseases and opportunistic pathogens. Seven bacteria and eight fungi were tested. The essential oil major compounds were apiol, myristicin, and beta-phellandrene. Parsley essential oil had bacteriostatic activity against all tested bacteria, mainly Staphylococcus aureus, Listeria monocytogenes, and Salmonella enterica, at similar or lower concentrations than at least one of the controls, and bactericidal activity against all tested bacteria, mainly S. aureus, at similar or lower concentrations than at least one of the controls. This essential oil also had fungistatic activity against all tested fungi, mainly, Penicillium ochrochloron and Trichoderma viride, at lower concentrations than the ketoconazole control and fungicidal activity against all tested fungi at higher concentrations than the controls. Parsley is used in cooking and medicine, and its essential oil is an effective antimicrobial agent