Association between principal component factor loading coefficients for PC1 (thick lines) and PC2 (thin lines).

<p>Coefficients are given for (a) C-females (n = 16) and T-females (n = 16; week₁₀: n = 15) before implantation and one, two, three, four, five and ten weeks after implantation and (b) C- and T-females ten weeks after implantation (n_C = 16, n_T = 15) and untreated males (n = 40).</p

Cere color of T- and C-females.

<p>Reflectance spectrophotometry results for the cere color of C-females (n = 16, gray line) and T-females (n = 16; week₁₀: n = 15, black line) before implantation (a), one (b), two (c), three (d), four (e), five (f) and ten (g) weeks after implantation.</p

Diagram of the choice test apparatus used in the experiment.

<p>The dark gray lines represent the perches. The light gray squares represent the food and water containers. Dotted lines represent grated cage walls.</p

Average % of fearful behaviours in the fear of human-test (±SEM) for each genotype and sex of the <i>TSHR</i> chickens.

<p>Post-hoc analyses males: bars designated with different letters differ significantly at P<0.05.</p

Ruuskanen_data_10.1111_jav_00728

Ruuskanen_data_10.1111_jav_0072

Means (±SEM) of the variables measured in the open field (OF) and social reinstatement (SR) test.

<p>P-values show the effect of genotype within each sex for TSHR chickens (Kruskal-Wallis) and effect of breed for both sexes for RJF/WL chickens (Mann-Whitney).</p><p>Means (±SEM) of the variables measured in the open field (OF) and social reinstatement (SR) test.</p

Estimated marginal means for incubation time (±SEM) from first hatch for each genotype/breed.

<p>TSHR genotypes: d/d: homozygous for the domestic allele, w/w: homozygous for the wild type allel, and w/d: heterozygous. RJF: Red Junglefowl, WL: White Leghorn. Post-hoc analyses <i>TSHR</i>: bars designated with different letters differ significantly at P<0.05.</p

Average plasma concentration (±SEM) for each genotype and sex of the <i>TSHR</i> chickens.

<p>(a) Plasma T3 concentration (b) Plasma T4 concentration. Post-hoc analyses, T3 males and T4 females and males: bars designated with different letters differ significantly at P<0.05.</p

Heat map of differentially expressed transcripts involved in developmental processes in abdomen and tail region.

<p>Green indicates downregulated (Log2FC ≤ -0.585, FDR ≤ 0.05), and red indicates upregulated (Log2FC ≥ 0.585, FDR ≤ 0.05) transcripts. White boxes indicate that transcripts were not differentially expressed. Grey boxes indicate that transcripts were removed from analysis in a specific body part because FG < BG + 2SD (See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123285#sec002" target="_blank">Materials and Methods</a>). Abd: abdomen, D1D2MO: combination of deiodinase type 1 and type 2 morpholino-injected, D3MO: deiodinase type 3 morpholino-injected. All differentially expressed transcripts are listed with their details in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123285#pone.0123285.s002" target="_blank">S2 Table</a>.</p

Ruuskanen: heritability of egg components

Ruuskanen: heritability of egg component