Nitrogen sources on TPOMW valorization through solid state fermentation performed by Yarrowia lipolytica

This manuscript reports the valorization of two-phase olive mill waste (TPOMW) as raw material and carbon source for solid state fermentation using Yarrowia lipolytica as biocatalyst. Due to its chemical characteristics, a combination of different raw materials (TPOMW and wheat bran, WB) was evaluated and two distinct nitrogen sources were applied as supplementation for lipase production. A TPOMW/WB ratio of 1:1 and supplementation with ammonium sulfate was chosen as the best condition. The productivity in 24 h reached 7.8 U/gh and, after four days of process, only decreased about 35%. Process pH ranged from 5.5-5.9, remaining in an acid range. Thus, the successful use of TPOMW, a watery solid by-product with high content of lipids, as raw material for Yarrowia lipolytica growth and lipase production provided an environmental friendly alternative to valorize such waste.The authors kindly acknowledge the financial aid and research scholarships given by CAPES. Maria Alice Zarur Coelho thanks CNPq (Proc. 308890/ 2013-2)

Evaluation of a quality improvement intervention to reduce anastomotic leak following right colectomy (EAGLE): pragmatic, batched stepped-wedge, cluster-randomized trial in 64 countries

Background: Anastomotic leak affects 8 per cent of patients after right colectomy with a 10-fold increased risk of postoperative death. The EAGLE study aimed to develop and test whether an international, standardized quality improvement intervention could reduce anastomotic leaks. Methods: The internationally intended protocol, iteratively co-developed by a multistage Delphi process, comprised an online educational module introducing risk stratification, an intraoperative checklist, and harmonized surgical techniques. Clusters (hospital teams) were randomized to one of three arms with varied sequences of intervention/data collection by a derived stepped-wedge batch design (at least 18 hospital teams per batch). Patients were blinded to the study allocation. Low- and middle-income country enrolment was encouraged. The primary outcome (assessed by intention to treat) was anastomotic leak rate, and subgroup analyses by module completion (at least 80 per cent of surgeons, high engagement; less than 50 per cent, low engagement) were preplanned. Results: A total 355 hospital teams registered, with 332 from 64 countries (39.2 per cent low and middle income) included in the final analysis. The online modules were completed by half of the surgeons (2143 of 4411). The primary analysis included 3039 of the 3268 patients recruited (206 patients had no anastomosis and 23 were lost to follow-up), with anastomotic leaks arising before and after the intervention in 10.1 and 9.6 per cent respectively (adjusted OR 0.87, 95 per cent c.i. 0.59 to 1.30; P = 0.498). The proportion of surgeons completing the educational modules was an influence: the leak rate decreased from 12.2 per cent (61 of 500) before intervention to 5.1 per cent (24 of 473) after intervention in high-engagement centres (adjusted OR 0.36, 0.20 to 0.64; P < 0.001), but this was not observed in low-engagement hospitals (8.3 per cent (59 of 714) and 13.8 per cent (61 of 443) respectively; adjusted OR 2.09, 1.31 to 3.31). Conclusion: Completion of globally available digital training by engaged teams can alter anastomotic leak rates. Registration number: NCT04270721 (http://www.clinicaltrials.gov)

Food Consumption Pattern and Dietary Diversity in Rural-urban Interface of Bangalore

The paper has examined the pattern of food consumption, dietary diversity and factors influencing dietary diversity across rural-urban interface of Bangalore. The study is based on the primary data of 510 households comprising of 189 rural, 211 transition and 110 urban households. Simpson Index of Dietary Diversity (SIDD) was employed to estimate the diversity in the consumption basket and to determine the factors influencing dietary diversity, the fractional probit model was used. The results showed that, higher cereal consumption was observed in rural area than in transition and urban areas and cereals were the prominent source of energy across all the gradients. The total calorie intake to the recommended calorie intake in urban, transition and rural area indicated that, the calorie intake in urban area was higher than the recommended intake (2100 Cal/CU/day) while, the scenario was opposite in transition and rural areas. This necessitates interventions to educate households to modify the existing purchasing behavior to reduce the gap between recommended and actual calorie intake. Further, the dietary diversity was analysed using SIDD, among the gradients, highest dietary diversity score was observed in urban (0.82) followed by transition (0.79) and rural gradients (0.77). Factors such as per capita income, access to irrigation and urban area had positive influence on dietary diversity. While, family size had negative influence on dietary diversity. Furthermore, among the different food items, cereals took major share in quantity consumption, calorie consumption and food consumption expenditure. On the other hand, diversity in consumption basket was marginally high in urban area. </jats:p

Council for High Blood Pressure Research/InterAmerican Society of Hypertension/International Society of Hypertension: First New Investigators Symposium at the High Blood Pressure Research 2011 Scientific Sessions

C5 - Other Refereed Contribution to Refereed Journal