The Impact of the COVID-19 Pandemic on K-12 School Teachers\u27 Stress, Coping and Burnout: Identification of Protective and Exacerbating Factors

The COVID-19 pandemic caused many changes and challenges for K-12 teachers and students alike. The changes in teaching modality, need for safety precautions, and challenges in maintaining contact with students contributed to heightened stress levels for teachers (Jones, 2020). Job stress has the potential to lead to burnout among teachers (de Vera Garcia & Gambarte, 2019). The present study sought to survey the stressors experienced by K-12 teachers in the U.S., the impact of teachers’ job-related stress on their burnout, and factors, such as resiliency, coping styles, resources, and social support, that might buffer against the effects of stress on burnout. Results of a quantitative survey (n = 74) and qualitative interview (n = 4) revealed that most teachers reported a moderate level of stress and burnout while teaching during the pandemic. Stress was significantly correlated with burnout, and teachers’ burnout-related exhaustion was higher than their disengagement. Resiliency, but not coping or social support, served to buffer against the negative effects of stress on burnout. Many participants found that they did have necessary resources for their students, but not enough support as educators. Overall findings suggest teachers were feeling stress and burnout despite any coping skills, social support or resilience

Conclusion: Message Control at the Margins

In the introduction, we identified four key themes that would guide the series of independent yet intersecting analyses that would follow. As those themes were explored and answers were provided to the questions being asked, a series of lessons for operating in the social-media-driven political environment emerged. These lessons shape the larger conclusions that can be derived from the collective efforts of the analyses herein

Introduction: Controlling the Message in the Social Media Marketplace of Ideas

The presidential candidate\u27s campaign faced the threat of being derailed following a scathing depiction of him posted by an individual citizen. Regardless of whether the claims made against the candidate were truthful, the message already had gone viral, and the candidate\u27s campaign flailed in its efforts to respond. Finally, one of the candidate\u27s supporters not affiliated with his campaign repackaged the critic\u27s depiction into a new theme, one that resonated positively with voters. The repackaged message itself continued well beyond its original posting as it was replicated in different forums time and time again

The writing on the (Facebook) wall: the use of social networking sites in hiring decisions

Abstract The popular media has reported an increase in the use of social networking sites (SNSs) such as Facebook by hiring managers and human resource professionals attempting to find more detailed information about job applicants. Within the peer-reviewed literature, cursory empirical evidence exists indicating that others' judgments of characteristics or attributes of an individual based on information obtained from SNSs may be accurate. Although this predictor method provides a potentially promising source of applicant information on predictor constructs of interest, it is also fraught with potential limitations and legal challenges. The level of publicly available data obtainable by employers is highly unstandardized across applicants, as some applicants will choose not to use SNSs at all while those choosing to use SNSs customize the degree to which information they share is made public to those outside of their network. It is also unclear how decision makers are currently utilizing the available information. Potential discrimination may result through employer's access to publicly available pictures, videos, biographical information, or other shared information that often allows easy identification of applicant membership to a protected class. For the practice to progress in a positive direction, evidence for the validity and job-relevance of information obtained from SNSs needs to be established. Organizational researchers and practitioners also need to promote awareness and attempt to create safeguards against the potential negative outcomes related to misuse of SNSs by employers

Evolved Resistance to a Novel Cationic Peptide Antibiotic Requires High Mutation Supply

Background and Objectives A key strategy for resolving the antibiotic resistance crisis is the development of new drugs with antimicrobial properties. The engineered cationic antimicrobial peptide WLBU2 (also known as PLG0206) is a promising broad-spectrum antimicrobial compound that has completed Phase I clinical studies. It has activity against Gram-negative and Gram-positive bacteria including infections associated with biofilm. No definitive mechanisms of resistance to WLBU2 have been identified. Methodology Here, we used experimental evolution under different levels of mutation supply and whole genome sequencing (WGS) to detect the genetic pathways and probable mechanisms of resistance to this peptide. We propagated populations of wild-type and hypermutator Pseudomonas aeruginosa in the presence of WLBU2 and performed WGS of evolved populations and clones. Results Populations that survived WLBU2 treatment acquired a minimum of two mutations, making the acquisition of resistance more difficult than for most antibiotics, which can be tolerated by mutation of a single target. Major targets of resistance to WLBU2 included the orfN and pmrB genes, previously described to confer resistance to other cationic peptides. More surprisingly, mutations that increase aggregation such as the wsp pathway were also selected despite the ability of WLBU2 to kill cells growing in a biofilm. Conclusions and implications The results show how experimental evolution and WGS can identify genetic targets and actions of new antimicrobial compounds and predict pathways to resistance of new antibiotics in clinical practice

Validation of ISS Floating Potential Measurement Unit Electron Densities and Temperatures

Validation of the Floating Potential Measurement Unit (FPMU) electron density and temperature measurements is an important step in the process of evaluating International Space Station spacecraft charging issues .including vehicle arcing and hazards to crew during extravehicular activities. The highest potentials observed on Space Station are due to the combined VxB effects on a large spacecraft and the collection of ionospheric electron and ion currents by the 160 V US solar array modules. Ionospheric electron environments are needed for input to the ISS spacecraft charging models used to predict the severity and frequency of occurrence of ISS charging hazards. Validation of these charging models requires comparing their predictions with measured FPMU values. Of course, the FPMU measurements themselves must also be validated independently for use in manned flight safety work. This presentation compares electron density and temperatures derived from the FPMU Langmuir probes and Plasma Impedance Probe against the independent density and temperature measurements from ultraviolet imagers, ground based incoherent scatter radar, and ionosonde sites

Survey of International Space Station Charging Events

With the negative grounding of the 160V Photovoltaic (PV) arrays, the International Space Station (ISS) can experience varied and interesting charging events. Since August 2006, there has been a multi-probe p ackage, called the Floating Potential Measurement Unit (FPMU), availa ble to provide redundant measurements of the floating potential of th e ISS as well as the density and temperature of the local plasma environment. The FPMU has been operated during intermittent data campaigns since August 2006 and has collected over 160 days of information reg arding the charging of the ISS as it has progressed in configuration from one to three PV arrays and with various additional modules such as the European Space Agency?s Columbus laboratory and the Japan Aeros pace Exploration Agency's Kibo laboratory. This paper summarizes the charging of the ISS and the local environmental conditions that contr ibute to those charging events, both as measured by the FPMU

Upward revision of global fossil fuel methane emissions based on isotope database

Methane has the second-largest global radiative forcing impact of anthropogenic greenhouse gases after carbon dioxide, but our understanding of the global atmospheric methane budget is incomplete. The global fossil fuel industry (production and usage of natural gas, oil and coal) is thought to contribute 15 to 22 per cent of methane emissions to the total atmospheric methane budget. However, questions remain regarding methane emission trends as a result of fossil fuel industrial activity and the contribution to total methane emissions of sources from the fossil fuel industry and from natural geological seepage, which are often co-located. Here we re-evaluate the global methane budget and the contribution of the fossil fuel industry to methane emissions based on long-term global methane and methane carbon isotope records. We compile the largest isotopic methane source signature database so far, including fossil fuel, microbial and biomass-burning methane emission sources. We find that total fossil fuel methane emissions (fossil fuel industry plus natural geological seepage) are not increasing over time, but are 60 to 110 per cent greater than current estimates owing to large revisions in isotope source signatures. We show that this is consistent with the observed global latitudinal methane gradient. After accounting for natural geological methane seepage, we find that methane emissions from natural gas, oil and coal production and their usage are 20 to 60 per cent greater than inventories. Our findings imply a greater potential for the fossil fuel industry to mitigate anthropogenic climate forcing, but we also find that methane emissions from natural gas as a fraction of production have declined from approximately 8 per cent to approximately 2 per cent over the past three decades.Published88-916A. Geochimica per l'ambienteJCR Journa

Hif-2α-Dependent Induction of miR-29a Restrains TH1 Activity During T Cell Dependent Colitis

Metabolic imbalance leading to inflammatory hypoxia and stabilization of hypoxia-inducible transcription factors (HIFs) is a hallmark of inflammatory bowel diseases. We hypothesize that HIF could be stabilized in CD4+ T cells during intestinal inflammation and alter the functional responses of T cells via regulation of microRNAs. Our assays reveal markedly increased T cell-intrinsic hypoxia and stabilization of HIF protein during experimental colitis. microRNA screen in primary CD4+ T cells points us towards miR-29a and our subsequent studies identify a selective role for HIF-2α in CD4-cell-intrinsic induction of miR-29a during hypoxia. Mice with T cell-intrinsic HIF-2α deletion display elevated T-bet (target of miR-29a) levels and exacerbated intestinal inflammation. Mice with miR-29a deficiency in T cells show enhanced intestinal inflammation. T cell-intrinsic overexpression of HIF-2α or delivery of miR-29a mimetic dampen TH1-driven colitis. In this work, we show a previously unrecognized function for hypoxia-dependent induction of miR-29a in attenuating TH1-mediated inflammation

Comparison of the structure and activity of glycosylated and asglycosylated human carboxylesterase 1

Human Carboxylesterase 1 (hCES1) is the key liver microsomal enzyme responsible for detoxification and metabolism of a variety of clinical drugs. To analyse the role of the single N-linked glycan on the structure and activity of the enzyme, authentically glycosylated and aglycosylated hCES1, generated by mutating asparagine 79 to glutamine, were produced in human embryonic kidney cells. Purified enzymes were shown to be predominantly trimeric in solution by analytical ultracentrifugation. The purified aglycosylated enzyme was found to be more active than glycosylated hCES1 and analysis of enzyme kinetics revealed that both enzymes exhibit positive cooperativity. Crystal structures of hCES1 a catalytically inactive mutant (S221A) and the aglycosylated enzyme were determined in the absence of any ligand or substrate to high resolutions (1.86 Å, 1.48 Å and 2.01 Å, respectively). Superposition of all three structures showed only minor conformational differences with a root mean square deviations of around 0.5 Å over all Cα positions. Comparison of the active sites of these un-liganded enzymes with the structures of hCES1-ligand complexes showed that side-chains of the catalytic triad were pre-disposed for substrate binding. Overall the results indicate that preventing N-glycosylation of hCES1 does not significantly affect the structure or activity of the enzyme