Supplementary data for article: Djokic, L.; Spasic, J.; Jeremic, S.; Vasiljevic, B.; Prodanovic, O.; Prodanovic, R.; Nikodinovic-Runic, J. Immobilization of Escherichia Coli Cells Expressing 4-Oxalocrotonate Tautomerase for Improved Biotransformation of β-Nitrostyrene. Bioprocess and biosystems engineering 2015, 38 (12), 2389–2395. https://doi.org/10.1007/s00449-015-1474-8

Supplementary material for: [https://doi.org/10.1007/s00449-015-1474-8]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1990

Supplementary data for article: Djokic, L.; Spasic, J.; Jeremic, S.; Vasiljevic, B.; Prodanovic, O.; Prodanovic, R.; Nikodinovic-Runic, J. Immobilization of Escherichia Coli Cells Expressing 4-Oxalocrotonate Tautomerase for Improved Biotransformation of β-Nitrostyrene. Bioprocess and biosystems engineering 2015, 38 (12), 2389–2395. https://doi.org/10.1007/s00449-015-1474-8

Supplementary material for: [https://doi.org/10.1007/s00449-015-1474-8]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1990

Actual contamination of the Danube and Sava Rivers at Belgrade (2013)

This study was focussed on a comprehensive investigation on the state of pollution of the Danube and Sava Rivers in the region of Belgrade. Different complementary analytical approaches were employed covering both i) organic contaminants in the river water by target analyses of hormones and neonicotinoids as well as non-target screening analyses and ii) heavy metals in the sediments. Finally, some common water quality parameters were analysed. The overall state of pollution is on a moderate level. Bulk parameters did not reveal any unusual observations. Moreover, quantification of preselected organic contaminants did not indicate to elevated pollution. More significant contaminations were registered for chromium, nickel, zinc and partially copper in sediments with values above the target values according to Serbian regulations. Lastly, non-target screening analysis revealed a wider spectrum of organic contaminants comprising pharmaceuticals, technical additives, personal care products and pesticides. The study presented a comprehensive view on the state of pollution of the Sava and Danube Rivers and is the base for setting up further monitoring programs. As a superior outcome, it was illustrated how different chemical analyses can result in different assessments of the river quality. A comparison of target and non-target analyses pointed to potential misinterpretation of the real state of pollution

Energetska i ekonomska optimizacija sistema toplotnog i fotonaponskog korišćenja sunčeve energije u specijalnoj bolnici u Banji Rusandi

In this paper are presented the results of optimization of thermal and photovoltaic system of solar energy utilization aimed to reduce CO2 emission by energy efficient and cost-effective replacing fossil fuel and electricity with solar energy for heating of water for different purposes (for pools sanitary water, washing) in the Special hospital of the Spa Rusanda -Melenci. Crucial for success of the study is understanding and prediction of solar water heating systems dynamic behaviour and synergetic relations between energy efficiency, solar radiation intensity stochastic changes, and sanitary water consumption dynamics. Prediction and analysis of relevant subsystems dynamics was basis for the optimisation of the TES - Thermal Energy Storage role within the system energy efficiency optimisation. In addition, the possible integration of the waste water heat utilization using heat-pump energy recovery system and reheating using heat pump powered by the solar PV electricity are analyzed. Although, the solar water heating has much higher efficiency (thermal - 4 to 5 times higher than PV) the evaluation of given new technical solution confirmed its high energy efficiency and cost effectiveness. Necessary alternative energy, during the periods when there is not enough solar radiation, can be efficiently provided concerning the possibility to cover nearly 80-90% of water heating needs, if water waste heat and TES are used as a heat pump source.U radu su izloženi rezultati studije i idejnog projekta urađenog u okviru Programa obnovljivih izvora energije srpske Agencije za energetsku efikasnost - SEEA. Data su tehnička rešenja i optimizacija sistema toplotnog i fotonaponskog korišćenja energije sunčevog zračenja za grejanje vode za različite namene (bazene, sanitarnu vodu, pranje veša i kuhinja) u Specijalnoj bolnici Rusanda - Melenci, u cilju smanjenja emisije ugljen-dioksida. Pokazano je da se zamena fosilnih goriva i električne energije može uspešno ostvariti putem energetski efikasnog i ekonomičnog korišćenja sunčeve energije. Od posebne važnosti za postizanje željenog cilja je razumevanje i predviđanje dinamičkog ponašanja sistema za grejanje vode sunčevom energijom i sinergetskih veza energetske efikasnosti, dinamike promena intenziteta sunčevog zračenja i dinamike potrošnje tople vode. Analizirana je moguća integracija primene toplote otpadne vode primenom toplotne pumpe i sistema povraćaja toplote i daljeg dogrevanja toplotnom pumpom napajanom električnom energijom proizvedenom fotonaponskim sistemom. Iako sistem za grejanje vode sunčevom energijom ima veću efikasnost (toplotno pretvaranje je 4 do 5 puta veće od fotonaponskog), utvrđeno je da se primenom novog tehničkog rešenja (sprega povraćaja toplote, toplotne pumpe i fotonaponskog sistema) može postići efektivnost i ekonomičnost sistema primene fotonaponskog pretvaranja potpuno uporedivog reda veličine sa toplotnim pretvaranjem sunčeve energije

Immobilization of Escherichia coli cells expressing 4-oxalocrotonate tautomerase for improved biotransformation of beta-nitrostyrene

The enzyme 4-oxalocrotonate tautomerase (4-OT) encoded by the xylH gene is a part of the degradation pathway of aromatic compounds in Pseudomonas putida mt-2. 4-OT was described to catalyze Michael-type addition of acetaldehyde to beta-nitrostyrene, and the whole cell system based on recombinantly expressed 4-OT has been developed previously. In this study biocatalytic process based on Escherichia coli whole cells expressing 4-OT was significantly improved using immobilization and ex situ product recovery strategies. Whole cell immobilization in alginate beads was applied in biocatalytic production of 4-nitro-3-phenyl-butanal from beta-nitrostyrene and acetaldehyde. Immobilized biocatalyst showed wider pH activity range and could tolerate twofold higher initial concentrations of substrate in comparison to the free whole cell biocatalyst. Beads retained their initial activity over 10 consecutive biotransformations of the model reaction and remained suitable for the repetitive use with 85 % of the initial activity after two months of storage. Bioprocess was further improved by utilizing Amberlite XAD-2 hydrophobic resin for the product recovery. With this modification, the amount of organic solvent was reduced 40-fold in comparison to previously reported method making this biocatalytic process greener

Immobilization of Escherichia coli cells expressing 4-oxalocrotonate tautomerase for improved biotransformation of beta-nitrostyrene

The enzyme 4-oxalocrotonate tautomerase (4-OT) encoded by the xylH gene is a part of the degradation pathway of aromatic compounds in Pseudomonas putida mt-2. 4-OT was described to catalyze Michael-type addition of acetaldehyde to beta-nitrostyrene, and the whole cell system based on recombinantly expressed 4-OT has been developed previously. In this study biocatalytic process based on Escherichia coli whole cells expressing 4-OT was significantly improved using immobilization and ex situ product recovery strategies. Whole cell immobilization in alginate beads was applied in biocatalytic production of 4-nitro-3-phenyl-butanal from beta-nitrostyrene and acetaldehyde. Immobilized biocatalyst showed wider pH activity range and could tolerate twofold higher initial concentrations of substrate in comparison to the free whole cell biocatalyst. Beads retained their initial activity over 10 consecutive biotransformations of the model reaction and remained suitable for the repetitive use with 85 % of the initial activity after two months of storage. Bioprocess was further improved by utilizing Amberlite XAD-2 hydrophobic resin for the product recovery. With this modification, the amount of organic solvent was reduced 40-fold in comparison to previously reported method making this biocatalytic process greener.Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3377

Formation of oxygen complexes in controlled atmosphere at surface of doped glassy carbon

The effects of boron and phosphorus incorporation in phenolic resin precursor to the oxidation resistance of glassy carbon have been studied. In order to reveal the nature and composition of the oxygen complexes formed at the surface of doped glassy carbon, under controlled atmosphere, the surface of the samples was cleaned under vacuum up to 1273 K. Specific functional groups, subsequently formed under dry CO2 or O-2 atmosphere on the surface of boron-doped and phosphorus-doped glassy carbon samples, were examined using the temperature-programmed desorption method combined with mass spectrometric analysis. Characterization of surface properties of undoped and doped samples has shown that in the presence of either boron or phosphorus heteroatoms, a lower amount of oxygen complexes formed after CO2 exposure, while, typically, higher amount of oxygen complexes formed after O-2 exposure. It has been concluded that the surface of undoped glassy carbon has a greater affinity towards CO2, while in the presence of either boron or phosphorus heteroatoms, the glassy carbon surface affinity becomes greater towards O-2, under experimental conditions