High resolution mapping of restoration of fertility (Rf) by combining large population and high density genetic map in pigeonpea [Cajanus cajan (L.) Millsp]

Background: Restoration of fertility (Rf) is an important trait for pigeonpea hybrid breeding. Few coarse quantitative trait locus (QTL) studies conducted in the past identified QTLs with large confidence intervals on the genetic map and could not provide any information on possible genes responsible for Rf in pigeonpea. Therefore, a larger population comprising of 369 F2s derived from ICPA 2039 × ICPL 87119 was genotyped with high density Axiom Cajanus SNP Array with 56 K single nucleotide polymorphism (SNPs) for high resolution mapping of Rf. Results: A genetic map with 4867 markers was developed and a total of four QTLs for Rf were identified. While one major effect QTL (qRf8.1) was co-localized with the QTL identified in two previous studies and its size was refined from 1.2 Mb to 0.41 Mb. Further analysis of qRf8.1 QTL with genome sequence provided 20 genes including two genes namely flowering locus protein T and 2-oxoglutarate/Fe (II)-dependent dioxygenases (2-ODDs) superfamily protein with known function in the restoration of fertility. Conclusion: The qRf8.1 QTL and the potential candidate genes present in this QTL will be valuable for genomicsassisted breeding and identification of causal genes/nucleotides for the restoration of fertility in the hybrid breeding program of pigeonpea

Optimum Power Randomization for the Minimization of Outage Probability

Cataloged from PDF version of article.The optimum power randomization problem is studied to minimize outage probability in flat block-fading Gaussian channels under an average transmit power constraint and in the presence of channel distribution information at the transmitter. When the probability density function of the channel power gain is continuously differentiable with a finite second moment, it is shown that the outage probability curve is a nonincreasing function of the normalized transmit power with at least one inflection point and the total number of inflection points is odd. Based on this result, it is proved that the optimum power transmission strategy involves randomization between at most two power levels. In the case of a single inflection point, the optimum strategy simplifies to on-off signaling for weak transmitters. Through analytical and numerical discussions, it is shown that the proposed framework can be adapted to a wide variety of scenarios including log-normal shadowing, diversity combining over Rayleigh fading channels, Nakagami-m fading, spectrum sharing, and jamming applications. We also show that power randomization does not necessarily improve the outage performance when the finite second moment assumption is violated by the power distribution of the fading. © 2013 IEEE