Heterotrophy as a tool to overcome the long and costly autotrophic scale-up process for large scale production of microalgae

Industrial scale-up of microalgal cultures is often a protracted step prone to culture collapse and the occurrence of unwanted contaminants. To solve this problem, a two-stage scale-up process was developed - heterotrophically Chlorella vulgaris cells grown in fermenters (1st stage) were used to directly inoculate an outdoor industrial autotrophic microalgal production unit (2nd stage). A preliminary pilot-scale trial revealed that C. vulgaris cells grown heterotrophically adapted readily to outdoor autotrophic growth conditions (1-m3 photobioreactors) without any measurable difference as compared to conventional autotrophic inocula. Biomass concentration of 174.5 g L-1, the highest value ever reported for this microalga, was achieved in a 5-L fermenter during scale-up using the heterotrophic route. Inocula grown in 0.2- and 5-m3 industrial fermenters with mean productivity of 27.54 ± 5.07 and 31.86 ± 2.87 g L-1 d-1, respectively, were later used to seed several outdoor 100-m3 tubular photobioreactors. Overall, all photobioreactor cultures seeded from the heterotrophic route reached standard protein and chlorophyll contents of 52.18 ± 1.30% of DW and 23.98 ± 1.57 mg g-1 DW, respectively. In addition to providing reproducible, high-quality inocula, this two-stage approach led to a 5-fold and 12-fold decrease in scale-up time and occupancy area used for industrial scale-up, respectively.Agência financiadora project FERMALG 017608 Fundacao para a Ciencia e a Tecnologia (FCT) UID/Multi/04326/2019 project FERMALG (AVISO) 32/SI/2015info:eu-repo/semantics/publishedVersio

Optical Surface Photometry of a Sample of Disk Galaxies. II Structural Components

This work presents the structural decomposition of a sample of 11 disk galaxies, which span a range of different morphological types. The U, B, V, R, and I photometric information given in Paper I (color and color-index images and luminosity, ellipticity, and position-angle profiles) has been used to decide what types of components form the galaxies before carrying out the decomposition. We find and model such components as bulges, disks, bars, lenses and rings.Comment: 14 figures. Accepted for publication in A&

Study of Plants Growth by Image Analysis

Analysis of plants growth is an important step for the evaluation of plants performance and productivity. This paper presents study of plants growth by image analysis from seeds and up to photosynthesis. To extract parameters two different segmentation methods are used, one for the seeds, and one for the plants, combined with morphological operations. The first image segmentation is achieved with Fuzzy C-Means clustering method and the second is achieved with entropy based method, using histogram evaluation. In tests, the segmented image is transformed in binary image and the relevant regions are extracted after applying morphological operation. The following parameters are calculated: the projected surface, the contour’s perimeter, the seed’s mass center, the projected surface, and the cotyledon’s and hyper cotyledon’slength for the plants

Towards Autopoietic Computing

A key challenge in modern computing is to develop systems that address complex, dynamic problems in a scalable and efficient way, because the increasing complexity of software makes designing and maintaining efficient and flexible systems increasingly difficult. Biological systems are thought to possess robust, scalable processing paradigms that can automatically manage complex, dynamic problem spaces, possessing several properties that may be useful in computer systems. The biological properties of self-organisation, self-replication, self-management, and scalability are addressed in an interesting way by autopoiesis, a descriptive theory of the cell founded on the concept of a system's circular organisation to define its boundary with its environment. In this paper, therefore, we review the main concepts of autopoiesis and then discuss how they could be related to fundamental concepts and theories of computation. The paper is conceptual in nature and the emphasis is on the review of other people's work in this area as part of a longer-term strategy to develop a formal theory of autopoietic computing.Comment: 10 Pages, 3 figure