Egr1 regulates the coordinated expression of numerous EGF receptor target genes as identified by ChIP-on-chip

UV stimulation of prostate cells causes an apoptotic response that is dependent on the zinc finger transcription factor Egr1; downstream targets of Egr1 in this response were identified

Optimizing Teaching Effectiveness in Dental Education for a New Generation of Learners

PurposeToday’s dental students, Generation Z (Gen Z), are said to learn differently than those of previous generations. As generations of dental students vary, our teaching styles must keep up with unique and changing groups of individuals.MethodsThis article discusses learner-focused teaching methods including techniques that address the characteristics of Gen Z learners. Blended learning methods that combine online media with traditional face-to-face sessions, team-based learning, and a flipped classroom format have previously been suggested as ways to increase learning effectiveness and student satisfaction.ResultsIn this paper, the characteristics and preferences of Gen Z students are described along with the challenges they create with conventional teaching methods. An implementation strategy using principles from organizational agility and Bolman and Deal’s Four Frames Model is proposed for dental schools to transition to a more learner-centered teaching approach.ConclusionsThe suggested strategy can be customized and could be useful to schools that wish to enhance their teaching methods to meet the learning needs of Gen Z dental students and beyond.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/175870/1/jdd13108.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/175870/2/jdd13108_am.pd

Intravital endoscopic technology for real-time monitoring of inflammation caused in experimental periodontitis

We report a novel method for in situ imaging of microvascular permeability in inflamed gingival tissue, using state-of-the-art Cellvizio™ intravital endoscopic technology and a mouse model of ligature-induced periodontitis. The silk ligature was first placed at the upper left second molar. Seven days later, the ligature was removed, and the animals were intravenously injected with Evans blue. Evans blue dye, which selectively binds to blood albumin, was used to monitor the level of inflammation by monitoring vascular permeability in control non-diseased and ligature-induced experimental periodontitis tissue. More specifically, leakage of Evans blue-bound albumin from the micro-capillary to connective tissue indicates the state of inflammation occurring in the specific site. Evans blue leakage from blood vessels was imaged in situ by directly attaching the endoscope (mini Z tip) of the Cellvizio™ system to the gingival tissue without any surgical incision. Evans blue emission intensity was significantly elevated in gingiva of periodontitis lesions, but not control non-ligature placed gingiva, indicating that this technology can be used as a potential minimally invasive diagnostic tool to monitor the level of inflammation at the periodontal disease site. •Cellvizio™ is an intravital endoscopic technology.•An assay to image periodontal lesions using Cellvizio™ was developed.•Evans blue dye is a fluorescent marker of vasculitis in periodontal lesions.•The assay could detect Evans blue leakage into surrounding tissue in a minimally invasive manner