Theoretical Principles of In Vitro Selection Using Combinatorial Nucleic Acid Libraries

A new paradigm for drug discovery and biological research has developed from technologies that integrate combinatorial chemistry with rounds of selection and amplification, a technique called in vitro selection or systematic evolution of ligands by exponential enrichment (SELEX). This overview unit discusses nucleic acid libraries that can be used, affinity probability distributions, an equilibrium model for SELEX, and optimal conditions including concentrations and signalâ toâ noise ratios.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/143632/1/cpnc0901.pd

LNOx Estimates Directly from LIS Data

No abstract availabl

Radiative and Cloud Microphysical Effects of Forest Fire Smoke over North America and Siberia

Aerosol affects climate both through direct radiative effects and by indirect effects on cloud development. Absorbing aerosols have additional influence on the vertical temperature profile of the atmospheric column. Radiative effects of smoke are studied for the case of a Canadian smoke plume that blanketed the U.S. mid-Atlantic seaboard. Optical properties derived from aircraft in situ measurements demonstrate that the smoke formed a layer with a base 2 km above the surface, and absorptive heating in this layer could have strengthened and maintained the subsidence inversion responsible for the layer structure. An optical model of the smoke formed from a blend of aircraft and AERONET measurements allows retrieval of the smoke aerosol by satellite, so that comparisons are possible to measurements made by any instrument in the region. For this case an optical model based purely on AERONET measurements provides the best satellite retrieval of optical depth, but a model based mainly on aircraft measurements agreed best with spectrum wide-forcing measurements, demonstrating the dangers of a simple optical model for all retrievals. A study done in the Amazonian burning season demonstrates that sun/observation geometry is useful to control bias from shadowed and illuminated portions of clouds. Sub-pixel mixing of cloud and aerosol also produces bias that is minimized for optically thick clouds. Since such biases can never be fully eliminated, the only valid study is a comparison of two data sets with equivalent geometry and so, presumably, equal bias. Canada and Siberia were chosen so that forested areas are compared at the same latitudes. Summertime Canadian aerosol is primarily smoke, while Europe contributes a great deal of sulfate to Siberia aerosol. The average cloud droplet size was significantly smaller in Siberia, as expected from the higher sulfate load with greater activity as cloud condensation nuclei (CCN). The aerosol indirect effect on cloud microphysics increases with aerosol loading in both regions, but much more so in Canada. This is attributed to a large sulfate background in Siberia, so the addition of smoke makes a smaller percentage change to the amount of cloud CCN

Variation of a Lightning NOx Indicator for National Climate Assessment

In support of the National Climate Assessment (NCA) program, satellite Lightning Imaging Sensor (LIS) data is used to estimate lightning nitrogen oxides (LNOx) production over the southern portion of the conterminous US. The total energy of each flash is estimated by analyzing the LIS optical event data associated with each flash (i.e., event radiance, event footprint area, and derivable event range). The LIS detects an extremely small fraction of the total flash energy; this fraction is assumed to be constant apart from the variability associated with the flash optical energy detected across the narrow (0.909 nm) LIS band. The estimate of total energy from each flash is converted to moles of LNOx production by assuming a chemical yield of 10(17) molecules Joule(-1). The LIS-inferred variable LNOx production from each flash is summed to obtain total LNOx production, and then appropriately enhanced to account for LIS detection efficiency and LIS view time. Annual geographical plots and time series of LNOx production are provided for a 16 year period (1998-2013)

Fine Structure in Manhattan’s Daytime Urban Heat Island: A New Dataset

A street-level temperature and humidity dataset, with high resolution spatial and temporal components, has been created for the island of Manhattan, suitable for use by the urban health and modelling communities. It consists of a set of pedestrian measurements over the course of two summers converted into anomaly maps, and a set of ten light-post mounted installations measuring temperature, relative humidity, and illumination at three minute intervals over three months. The quality control and data reduction, used to produce the anomaly maps, is described, and the relationships between spatial and temporal variability are investigated. The data sets are available for download via the project Web site

Variation of a Lightning NOx Indicator for National Climate Assessment

During the past couple of years, an analysis tool was developed by the NASA Marshall Space Flight Center (MSFC) for the National Climate Assessment (NCA) program. The tool monitors and examines changes in lightning characteristics over the conterminous US (CONUS) on a continual basis. In this study, we have expanded the capability of the tool so that it can compute a new climate assessment variable that is called the Lightning NOx Indicator (LNI). Nitrogen oxides (NOx = NO + NO2) are known to indirectly influence our climate, and lightning NOx is the most important source of NOx in the upper troposphere (particularly in the tropics). The LNI is derived using Lightning Imaging Sensor (LIS) data and is computed by summing up the product of flash area x flash brightness over all flashes that occur in a particular region and period. Therefore, it is suggested that the LNI is a proxy to lightning NOx production. Specifically, larger flash areas are consistent with longer channel length and/or more energetic channels, and hence more NOx production. Brighter flashes are consistent with more energetic channels, and hence more NOx production. The location of the flash within the thundercloud and the optical scattering characteristics of the thundercloud are of course complicating factors. We analyze LIS data for the years 2003-2013 and provide geographical plots of the time-evolution of the LNI in order to determine if there are any significant changes or trends between like seasons, or from year to year

Heat-induced alterations in cashew allergen solubility and IgE binding

AbstractCashew nuts are an increasingly common cause of food allergy. We compare the soluble protein profile of cashew nuts following heating. SDS-PAGE indicate that heating can alter the solubility of cashew nut proteins. The 11S legumin, Ana o 2, dominates the soluble protein content in ready to eat and mildly heated cashew nuts. However, we found that in dark-roasted cashew nuts, the soluble protein profile shifts and the 2S albumin Ana o 3 composes up to 40% of the soluble protein. Analysis of trypsin-treated extracts by LC/MS/MS indicate changes in the relative number and intensity of peptides. The relative cumulative intensity of the 5 most commonly observed Ana o 1 and 2 peptides are altered by heating, while those of the 5 most commonly observed Ana o 3 peptides remaine relatively constant. ELISA experiments indicate that there is a decrease in rabbit IgG and human serum IgE binding to soluble cashew proteins following heating. Our findings indicate that heating can alter the solubility of cashew allergens, resulting in altered IgE binding. Our results support the use of both Ana o 2 and Ana o 3 as potential cashew allergen diagnostic targets

Variability of CONUS Lightning in 2003–12 and Associated Impacts

Changes in lightning characteristics over the conterminous United States (CONUS) are examined to support the National Climate Assessment (NCA) program. Details of the variability of cloud-to-ground (CG) lightning characteristics over the decade 2003–12 are provided using data from the National Lightning Detection Network (NLDN). Changes in total (CG + cloud flash) lightning across part of the CONUS during the decade are provided using satellite Lightning Imaging Sensor (LIS) data. The variations in NLDN-derived CG lightning are compared with available statistics on lightning-caused impacts to various U.S. economic sectors. Overall, a downward trend in total CG lightning count is found for the decadal period; the 5-yr mean NLDN CG count decreased by 12.8% from 25 204 345.8 (2003–07) to 21 986 578.8 (2008–12). There is a slow upward trend in the fraction and number of positive-polarity CG lightning, however. Associated lightning-caused fatalities and injuries, and the number of lightning-caused wildland fires and burn acreage also trended downward, but crop and personal-property damage costs increased. The 5-yr mean LIS total lightning changed little over the decadal period. Whereas the CONUS-averaged dry-bulb temperature trended upward during the analysis period, the CONUS-averaged wet-bulb temperature (a variable that is better correlated with lightning activity) trended downward. A simple linear model shows that climate-induced changes in CG lightning frequency would likely have a substantial and direct impact on humankind (e.g., a long-term upward trend of 1°C in wet-bulb temperature corresponds to approximately 14 fatalities and over $367 million in personal-property damage resulting from lightning)

Assessing Global Change Impact on the US Using National Lightning Data

No abstract availabl