Insilico analysis of Arabidopsis ferric reductase oxidases (FRO) proteins associated with iron homeostasis

The ferric reduction oxidase (FRO) gene family is involved in various biological processes of plants and plays an essential role in metal homeostasis, tolerance, and signaling networks in response to several abiotic stresses. Our study describes the structural, functional characterization, and evolutionary relationships of eight Arabidopsis FRO proteins. The studies predicted the subcellular localization of FRO proteins to the plasma membrane, mitochondria, and chloroplast organelles. The structural analysis revealed localization of proteins onto the first and fifth chromosomes having 8-9exons and 8-10 transmembrane helices. The protein features of FRO proteins revealed 699-747 amino acids having 79600.02-84126.3 (Da) molecular weight. The six highly conserved protein motifs were predicted with 45-50 amino acids long representing ferric chelate reductase family domains. The phylogeny tree constructed using Clustal W divided the FRO proteins into two clusters and the interactome network revealed the co-expression of COPT1, NRAMP1, NRAMP3, NRAMP4, FRD3, OPT3, IRT1, IRT2, ZIF1, PYE proteins along with the seven FRO proteins

Studies On Spatio-Temporal and Phytohormonal Expression Pattern of MTP2 Protein and its Characterization across Different Plant Species

Zinc is an essential micronutrient involved in a wide range of physiological processes of plants (Marschner, 1993). Even though it is required for plantgrowth, the excess amount of Zn causes toxic effects to plants. It is known that during Fe deficiency, the uptake of Zn is induced which leads to the accumulation of the excess amount of Zn in the plant cytoplasm. The plant has evolved an efficient mechanism in detoxifying the excess Zn uptake during Fe deficiency conditions, of which vacuoles are identified to be the main storage and detoxifying organelles for Zn excess in the cytoplasm (Gustin et al., 2009). Zinc is normally available to the plants in its oxidized form Zn2+ from the soil through the mass flow and diffusion mechanism by roots (Rattan and Deb, 1981). Availability of Zn is high in low acidic pH wherein the high pH reduces its availability in soil and the root plasma membrane transporters reported for major uptake of Zn from the soil (Marschner, 1993)

Rainbow refractrometry: On the validity domain of Airy's and Nussenzveig's theories

cited By 33International audienceno abstrac

Assessment of rickettsial diseases presenting as acute undifferentiated febrile illness using clinical tools in a government tertiary care teaching hospital, Chamarajanagar, Karnataka

Background: Rickettsial diseases are re-emerging in India. Acute undifferentiated febrile illness is a difficult case to treat (AUFI). The greatest problem for clinicians is the early diagnosis of these diseases when antibiotic therapy is most successful.To determine the prevalence of RDs, therapy outcomes, and socio-demographic factors associated with RDs in AUFI. Patients and Methods: Fever and/or clinical signs of Rickettsial infection in patients admitted to the CIMS teaching hospital in Chamarajanagar for four years. Purposive sampling found AUFI in 1638 people. The diagnosis of rickettsial disease relied on clinical features. The Weil-Felix test was one. Then came a 48–72-hour estimate of doxycycline response. Results:Out of 1810 AUFI cases, 198 (10.93%) were rickettsial, with 190 (95.95%) having an RGA score of 14 or more, and 18 (9.09%) having an RGA score of 14 or less. Males aged 30-40 were most affected. Leucopenia, thrombocytopenia, etc. were found. Doxycycline was effective in 151 (89.35%) of cases within one week of onset. The remaining 18 (10.65%) required longer-term doxycycline with other drugs, three had severe complications, and one died. RD prevalence 70.01 %. Enteric fever, Dengue fever, Chikungunya fever, and Rickettsial diseases were the most common diseases diagnosed in patients with undifferentiated febrile illness.&nbsp

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Not AvailableCastor is an industrially important oilseed crop, which provides raw material to many industries. Despite its industrial importance, the genomic resources available for molecular breeding applications are limited. In the present study, a set of 135 SNP loci polymorphic between two castor inbred lines namely, JC-12 and RG-1963 were targeted to develop competitive allele specific PCR assays for genotyping purposes. The assays were validated in the F2 population of JC-12 × RG-1963 cross. Out of 135 assays designed, 124 were found to be successful in discriminating three expected genotypes in the F2 population. Using the SNP genotyping data, a genetic linkage map representing 10 haploid chromosomes of castor was constructed, which corresponded very well with the physical map. This set of validated SNP markers is a useful resource for application in molecular breeding of castor.ICA

Rainbow refractrometry: On the validity domain of Airy's and Nussenzveig's theories

cited By 33International audienceno abstrac

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Not AvailableFor introgressing Brown planthopper (BPH) resistance into a rice variety using marker assisted selection, study of parental polymorphism between the donor and recipient parents is a pre-requisite step. Unless the parents are polymorphic for the BPH reaction, further selection of plants carrying the traits may not be possible. Thus, the present investigation was conducted to analyze the polymorphism of two BPH resistant donors (M229, 10-3) and a recipient (Telangana Sona) parent using SSR markers. A total of 494 random microsatellite markers evenly distributed across 12 chromosomes were used for the polymorphic survey. Among these, 87 markers were found polymorphic between M229 and Telangana Sona, while 93 were polymorphic between 10-3 and Telangana Sona by producing distinct reproducible amplification patterns. The highest percentage of polymorphism was observed on chromosome 12 (30.59%) for Telangana Sona and M229, while chromosome 1 (30.61%) recorded the highest polymorphism percentage for Telangana Sona and 10-3 indicating that these chromosomes are useful in studying the variation between the parents. Accordingly, the maximum number of polymorphic markers were obtained on chromosome 6 for the parents of both the crosses. The identified polymorphic markers across the 12 chromosomes will be useful in subsequent linkage map construction along with mapping of QTLs associated with BPH resistance.Not Availabl

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Not AvailableBrown planthopper (BPH), Nilaparvata lugens (Stål) is a destructive pest that poses a threat to the food security of rice producing countries. In order to breed rice varieties with resistance to the brown planthopper, it is necessary to identify highly resistant germplasm sources. Seventy-three genotypes were evaluated using standard seed box screening technique over a period of two seasons that have shown varied levels of response reactions to the planthopper. Simultaneously, fifty-three microsatellite markers linked to BPH resistance were employed to find their association with BPH resistance. This association was estimated using regression based Single Marker Analysis (SMA) in Microsoft Excel. The significant marker trait associations (MTAs) were indicated by a P-value (< 0.05) along with corresponding R2 for each marker explaining the total phenotypic variation for BPH resistance accounted by markers. Statistically significant marker-trait associations were observed for five of the 39 polymorphic markers viz, RM335, RM510, RM589, RM8213 and RM494 indicating the possibility of significant putative genetic loci associated with BPH resistance on chromosomes 4, 6 and 10. The percentage of the total phenotypic variation as explained by the significant markers ranged from 1.0% to 24.4% suggesting the reliability of these genetic markers for further improvement of breeding for BPH resistance. Since these markers are linked with Bph3, Bph17(t), QBph6 and QBph10 genes/QTLs respectively, the presence of these genes in the resistant germplasm lines found in the study could be exploited through marker-assisted breeding programs for the development of BPH resistant cultivars.Not Availabl