Detection of hidden gratings through multilayer nanostructures using light and sound

We report on the detection of diffraction gratings buried below a stack of tens of 18 nm thick $\mathrm{SiO_2}$ and $\mathrm{Si_3N_4}$ layers and an optically opaque metal layer, using laser-induced, extremely-high frequency ultrasound. In our experiments, the shape and amplitude of a buried metal grating is encoded on the spatial phase of the reflected acoustic wave. This grating-shaped acoustic echo from the buried grating is detected by diffraction of a delayed probe pulse. The shape and strength of the time-dependent diffraction signal can be accurately predicted using a 2D numerical model. Surprisingly, our numerical calculations show that the diffracted signal strength is not strongly influenced by the number of dielectric layers through which the acoustic wave has to propagate. Replacing the $\mathrm{SiO_2}$/$\mathrm{Si_3N_4}$ layer stack with a single layer having an equivalent time-averaged sound velocity and average density, has only a small effect on the shape and amplitude of the diffracted signal as a function of time. Our results show that laser-induced ultrasound is a promising technique for sub-surface nano-metrology applications

Nutrition in children with CRF and on dialysis

The objectives of this study are: (1) to understand the importance of nutrition in normal growth; (2) to review the methods of assessing nutritional status; (3) to review the dietary requirements of normal children throughout childhood, including protein, energy, vitamins and minerals; (4) to review recommendations for the nutritional requirements of children with chronic renal failure (CRF) and on dialysis; (5) to review reports of spontaneous nutritional intake in children with CRF and on dialysis; (6) to review the epidemiology of nutritional disturbances in renal disease, including height, weight and body composition; (7) to review the pathological mechanisms underlying poor appetite, abnormal metabolic rate and endocrine disturbances in renal disease; (8) to review the evidence for the benefit of dietetic input, dietary supplementation, nasogastric and gastrostomy feeds and intradialytic nutrition; (9) to review the effect of dialysis adequacy on nutrition; (10) to review the effect of nutrition on outcome

Role of scattering by surface roughness in the photoacoustic detection of hidden micro-structures

We present an experimental study in which we compare two different pump–probe setups to generate and detect high-frequency laser-induced ultrasound for the detection of gratings buried underneath optically opaque metal layers. One system is built around a high-fluence, low-repetition-rate femtosecond laser (1 kHz) and the other around a low-fluence, high-repetition-rate femtosecond laser (5.1 MHz). We find that the signal diffracted by the acoustic replica of the grating as a function of pump–probe time delay is very different for the two setups used. We attribute this difference to the presence of a constant background field due to optical scattering by interface roughness. In the low-fluence setup, the optical field diffracted by the acoustic replica is significantly weaker than the background optical field, with which it can destructively or constructively interfere. For the right phase difference between the optical fields, this can lead to a significant “amplification” of the weak field diffracted off the grating-shaped acoustic waves. For the high-fluence system, the situation is reversed because the field diffracted off the acoustic-wave-induced grating is significantly larger than the background optical field. Our measurements show that optical scattering by interface roughness must be taken into account to properly explain experiments on laser-induced ultrasound performed with high-repetition-rate laser systems and can be used to enhance signal strength

High-resolution microscopy through optically opaque media using ultrafast photoacoustics

We present a high-resolution microscope capable of imaging buried structures through optically opaque materials with micrometer transverse resolution and a nanometer-scale depth sensitivity. The ability to image through such materials is made possible by the use of laser ultrasonic techniques, where an ultrafast laser pulse launches acoustic waves inside an opaque layer and subsequent acoustic echoes from buried interfaces are detected optically by a time-delayed probe pulse. We show that the high frequency of the generated ultrasound waves enables imaging with a transverse resolution only limited by the optical detection system. We present the imaging system and signal analysis and demonstrate its imaging capability on complex microstructured objects through 200 nm thick metal layers and gratings through 500 nm thickness. Furthermore, we characterize the obtained imaging performance, achieving a diffraction-limited transverse resolution of 1.2 μm and a depth sensitivity better than 10 nm

Double active control of the plasmonic resonance of a gold nanoparticle array

A two-fold active control of the plasmonic resonance of randomly distributed gold nanoparticles (GNPs) has been achieved. GNPs have been immobilized on an Indium Tin Oxide (ITO) coated glass substrate and then covered with a liquid crystalline compound. The system has been investigated by means of atomic force and scanning electron microscopy, revealing the presence of isolated and well distributed GNPs. The application of an external electric field to the sample has a two-fold consequence: the re-orientation of the hybrid-aligned liquid crystal layer and the formation of a carrier accumulation layer in the proximity of the ITO substrate. The refractive indices of both liquid crystal and accumulation layers are influenced by the applied field in a competitive way and produce a “dancing behavior” of the GNP’s plasmonic resonance spectral position

Plasmonic enhancement of photoacoustic-induced reflection changes

In this paper, we report on surface-plasmon-resonance enhancement of the time-dependent reflection changes caused by laser-induced acoustic waves. We measure an enhancement of the reflection changes induced by several acoustical modes, such as longitudinal, quasi-normal, and surface acoustic waves, by a factor of 10–20. We show that the reflection changes induced by the longitudinal and quasi-normal modes are enhanced in the wings of the surface plasmon polariton resonance. The surface acoustic wave-induced reflection changes are enhanced on the peak of this resonance. We attribute the enhanced reflection changes to the longitudinal wave and the quasi-normal mode to a shift in the surface plasmon polariton resonance via acoustically induced electron density changes and via grating geometry changes

Plasmonic enhancement of photoacoustic-induced reflection changes

In this paper, we report on surface-plasmon-resonance enhancement of the time-dependent reflection changes caused by laser-induced acoustic waves.We measure an enhancement of the reflection changes induced by several acoustical modes, such as longitudinal, quasi-normal, and surface acoustic waves, by a factor of 10-20.We show that the reflection changes induced by the longitudinal and quasi-normal modes are enhanced in the wings of the surface plasmon polariton resonance. The surface acoustic wave-induced reflection changes are enhanced on the peak of this resonance.We attribute the enhanced reflection changes to the longitudinal wave and the quasi-normal mode to a shift in the surface plasmon polariton resonance via acoustically induced electron density changes and via grating geometry changes. ImPhys/Optic

Label-free and reusable antibody-functionalized gold nanorod arrays for the rapid detection of Escherichia coli cells in a water dispersion

The growing spread of pathogens, caused by anthropogenic activities, pushes the interest of the scientific community towards developing biosensors with improved performance for rapid, simple, and on-site pathogen detection. In this study, we present and discuss a label-free gold nanorod (Au NR) array for the rapid detection of Escherichia coli cells in water, resulting in an effective optical transducer, based on the phenomenon of localized surface plasmon resonance (LSPR). Au NRs with different aspect ratios are func- tionalized with a suitable antibody by an electrostatic-linking method, resulting in two different Au NR- based bioconjugates. We investigate the ability of the two bioconjugates to detect and spectroscopically recognize E. coli cells dispersed in water by specific antigen–antibody interaction. The results allow selecting the Au NR morphology more suited for preparing the Au NR bioactive array on a glass substrate with excellent optical and morphological properties. The antibody-functionalized Au NR array can detect E. coli cells with high sensitivity and a limit of detection of 8.4 CFU mL−1, resulting in an excellent label-free spectroscopic biosensor. In addition, the multicolor thermoplasmonic properties of the Au NR array, trig- gered by appropriate light sources, are suited to enable on-demand photothermal disinfection, thus pro- viding an extraordinary capacity for the biosensor to be both disinfected and, more importantly, reutilized